Single cell type - AMZ2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AMZ2
Gene descriptionⁱ Full gene name according to HGNC.	Archaelysin family metallopeptidase 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	14

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids, Astrocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids, Astrocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AMZ2 is part of cluster 25 Spermatids - Spermatogenesis with confidenceⁱ 1
458 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CHRNE	Cholinergic receptor nicotinic epsilon subunit	0.7416	25
LPIN1	Lipin 1	0.6993	25
C12orf54	Chromosome 12 open reading frame 54	0.6929	25
PCP2	Purkinje cell protein 2	0.6924	25
LRRD1	Leucine rich repeats and death domain containing 1	0.6869	25
TMEM239	Transmembrane protein 239	0.6825	25
CCDC169	Coiled-coil domain containing 169	0.6818	25
UBE2N	Ubiquitin conjugating enzyme E2 N	0.6817	25
ACSBG2	Acyl-CoA synthetase bubblegum family member 2	0.6803	25
C3orf22	Chromosome 3 open reading frame 22	0.6801	25
MROH7	Maestro heat like repeat family member 7	0.6793	25
CPT1B	Carnitine palmitoyltransferase 1B	0.6761	25
SH3RF2	SH3 domain containing ring finger 2	0.6749	25
C16orf82	Chromosome 16 open reading frame 82	0.6748	25
FSCN3	Fascin actin-bundling protein 3	0.6745	25

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	18.5 nTPM
Breast	19.4 nTPM
Adipose tissue	17.6 nTPM

Alveolar cells type 1	12.8 nTPM
Lung	12.8 nTPM

Alveolar cells type 2	15.6 nTPM
Lung	15.6 nTPM

B-cells	38.7 nTPM
Stomach	56.3 nTPM
Breast	49.4 nTPM
Liver	46.2 nTPM
PBMC	44.2 nTPM
Lymph node	36.8 nTPM
Spleen	35.7 nTPM
Adipose tissue	33.6 nTPM
Colon	31.9 nTPM
Kidney	28.5 nTPM
Bone marrow	24.5 nTPM

Basal keratinocytes	18.6 nTPM
Skin	18.6 nTPM

Dendritic cells	58.0 nTPM
Breast	62.0 nTPM
PBMC	59.8 nTPM
Adipose tissue	52.2 nTPM

Endothelial cells	24.4 nTPM
Heart muscle	37.0 nTPM
Testis	33.2 nTPM
Skin	28.0 nTPM
Pancreas	27.0 nTPM
Eye	25.9 nTPM
Liver	25.7 nTPM
Placenta	25.6 nTPM
Breast	25.4 nTPM
Lung	24.9 nTPM
Esophagus	24.2 nTPM
Ovary	19.7 nTPM
Skeletal muscle	19.4 nTPM
Endometrium	14.6 nTPM
Prostate	11.5 nTPM

Fibroblasts	28.2 nTPM
Heart muscle	49.5 nTPM
Placenta	34.9 nTPM
Skin	34.2 nTPM
Breast	30.1 nTPM
Esophagus	29.7 nTPM
Ovary	28.6 nTPM
Prostate	26.8 nTPM
Lung	23.4 nTPM
Skeletal muscle	22.5 nTPM
Adipose tissue	20.4 nTPM
Stomach	10.4 nTPM

Intestinal goblet cells	16.7 nTPM
Rectum	20.3 nTPM
Small intestine	19.1 nTPM
Colon	10.7 nTPM

Macrophages	39.0 nTPM
Breast	60.9 nTPM
Adipose tissue	60.2 nTPM
Prostate	57.3 nTPM
Skeletal muscle	48.4 nTPM
Testis	44.1 nTPM
Endometrium	41.0 nTPM
Pancreas	35.7 nTPM
Ovary	33.5 nTPM
Stomach	32.2 nTPM
Spleen	32.1 nTPM
Lung	31.9 nTPM
Kidney	31.0 nTPM
Esophagus	28.3 nTPM
PBMC	27.3 nTPM
Bone marrow	21.2 nTPM

Paneth cells	10.8 nTPM
Small intestine	15.0 nTPM
Rectum	14.3 nTPM
Colon	3.3 nTPM

Plasma cells	46.2 nTPM
Spleen	66.7 nTPM
Stomach	49.6 nTPM
Esophagus	43.0 nTPM
Bone marrow	36.2 nTPM
Adipose tissue	35.5 nTPM

Respiratory ciliated cells	61.8 nTPM
Lung	62.7 nTPM
Bronchus	61.0 nTPM

Smooth muscle cells	26.8 nTPM
Bronchus	44.3 nTPM
Pancreas	41.7 nTPM
Esophagus	32.5 nTPM
Heart muscle	30.0 nTPM
Skin	30.0 nTPM
Breast	28.6 nTPM
Adipose tissue	21.7 nTPM
Skeletal muscle	19.2 nTPM
Prostate	18.1 nTPM
Ovary	15.2 nTPM
Endometrium	13.7 nTPM

T-cells	49.3 nTPM
Skin	71.3 nTPM
Prostate	61.6 nTPM
Liver	60.6 nTPM
Stomach	60.0 nTPM
Esophagus	58.9 nTPM
Breast	56.0 nTPM
Kidney	50.7 nTPM
Bone marrow	49.1 nTPM
Spleen	49.1 nTPM
Lymph node	46.6 nTPM
Lung	45.6 nTPM
Ovary	44.5 nTPM
Colon	42.7 nTPM
Endometrium	39.3 nTPM
PBMC	37.5 nTPM
Skeletal muscle	32.4 nTPM
Adipose tissue	32.0 nTPM

Astrocytes	477.3 nTPM
Brain	477.3 nTPM

Cholangiocytes	21.5 nTPM
Liver	21.5 nTPM

Collecting duct cells	23.9 nTPM
Kidney	23.9 nTPM

Cone photoreceptor cells	8.4 nTPM
Eye	8.4 nTPM

Undifferentiated cells	15.1 nTPM
Small intestine	20.4 nTPM
Rectum	17.6 nTPM
Colon	7.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC