Single cell type - SPRED2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SPRED2
Gene descriptionⁱ Full gene name according to HGNC.	Sprouty related EVH1 domain containing 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Synaptic function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Excitatory neurons, Oligodendrocyte precursor cells, Inhibitory neurons, Astrocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (plasmacytoid DC, NK-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Excitatory neurons, Oligodendrocyte precursor cells, Inhibitory neurons, Astrocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

SPRED2 is part of cluster 8 Neurons & Oligodendrocytes - Synaptic function with confidenceⁱ 1
719 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TRIO	Trio Rho guanine nucleotide exchange factor	0.6558	8
PTPN9	Protein tyrosine phosphatase non-receptor type 9	0.6119	50
PTPRG	Protein tyrosine phosphatase receptor type G	0.5762	68
XKR6	XK related 6	0.5686	21
NTRK2	Neurotrophic receptor tyrosine kinase 2	0.5684	8
DPP10	Dipeptidyl peptidase like 10	0.5684	8
RAPGEF4	Rap guanine nucleotide exchange factor 4	0.5629	8
SOX5	SRY-box transcription factor 5	0.5552	8
SPRY3	Sprouty RTK signaling antagonist 3	0.5435	8
ALDH1L2	Aldehyde dehydrogenase 1 family member L2	0.5434	50
SPDYE17	Speedy/RINGO cell cycle regulator family member E17	0.5403	8
MARK1	Microtubule affinity regulating kinase 1	0.5375	8
SMYD3	SET and MYND domain containing 3	0.5371	8
PPARGC1A	PPARG coactivator 1 alpha	0.5345	8
GFRAL	GDNF family receptor alpha like	0.5303	14

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	49.7 nTPM
Adipose tissue	75.8 nTPM
Breast	23.5 nTPM

Alveolar cells type 1	19.2 nTPM
Lung	19.2 nTPM

Alveolar cells type 2	16.0 nTPM
Lung	16.0 nTPM

B-cells	1.8 nTPM
Colon	4.8 nTPM
Kidney	4.8 nTPM
Breast	2.6 nTPM
Lymph node	2.1 nTPM
Bone marrow	1.7 nTPM
Adipose tissue	1.0 nTPM
Liver	0.9 nTPM
Stomach	0.5 nTPM
PBMC	0.0 nTPM
Spleen	0.0 nTPM

Basal keratinocytes	39.9 nTPM
Skin	39.9 nTPM

Dendritic cells	3.3 nTPM
Adipose tissue	5.5 nTPM
Breast	4.3 nTPM
PBMC	0.0 nTPM

Endothelial cells	18.6 nTPM
Skeletal muscle	35.1 nTPM
Skin	33.2 nTPM
Prostate	27.7 nTPM
Ovary	25.1 nTPM
Breast	24.4 nTPM
Heart muscle	23.6 nTPM
Pancreas	19.3 nTPM
Endometrium	15.4 nTPM
Eye	10.5 nTPM
Testis	10.4 nTPM
Esophagus	9.7 nTPM
Lung	9.3 nTPM
Placenta	9.1 nTPM
Liver	8.2 nTPM

Fibroblasts	21.0 nTPM
Adipose tissue	43.0 nTPM
Skeletal muscle	37.3 nTPM
Skin	28.1 nTPM
Prostate	26.6 nTPM
Esophagus	25.3 nTPM
Heart muscle	20.1 nTPM
Breast	19.8 nTPM
Ovary	12.1 nTPM
Lung	11.1 nTPM
Placenta	4.9 nTPM
Stomach	3.0 nTPM

Intestinal goblet cells	24.1 nTPM
Rectum	26.3 nTPM
Colon	23.5 nTPM
Small intestine	22.4 nTPM

Macrophages	10.2 nTPM
Endometrium	23.2 nTPM
Adipose tissue	20.8 nTPM
Prostate	16.0 nTPM
Esophagus	14.2 nTPM
PBMC	13.6 nTPM
Skeletal muscle	13.6 nTPM
Testis	11.1 nTPM
Breast	7.5 nTPM
Kidney	6.8 nTPM
Bone marrow	6.2 nTPM
Stomach	5.9 nTPM
Spleen	5.2 nTPM
Lung	4.4 nTPM
Pancreas	2.5 nTPM
Ovary	2.0 nTPM

Paneth cells	21.5 nTPM
Colon	39.6 nTPM
Rectum	17.4 nTPM
Small intestine	7.5 nTPM

Plasma cells	0.5 nTPM
Adipose tissue	2.7 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Respiratory ciliated cells	7.2 nTPM
Lung	7.3 nTPM
Bronchus	7.1 nTPM

Smooth muscle cells	17.4 nTPM
Adipose tissue	36.1 nTPM
Heart muscle	24.0 nTPM
Skeletal muscle	23.1 nTPM
Skin	18.5 nTPM
Pancreas	17.8 nTPM
Prostate	16.2 nTPM
Endometrium	14.4 nTPM
Breast	11.0 nTPM
Esophagus	10.9 nTPM
Ovary	10.8 nTPM
Bronchus	8.6 nTPM

T-cells	3.8 nTPM
Esophagus	13.1 nTPM
Endometrium	11.7 nTPM
Skin	11.6 nTPM
Adipose tissue	5.5 nTPM
Lung	4.5 nTPM
Colon	3.8 nTPM
Skeletal muscle	3.5 nTPM
Breast	3.4 nTPM
Liver	2.4 nTPM
Stomach	2.4 nTPM
Lymph node	0.9 nTPM
Kidney	0.7 nTPM
Bone marrow	0.7 nTPM
Spleen	0.3 nTPM
PBMC	0.2 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Astrocytes	108.7 nTPM
Brain	108.7 nTPM

Cholangiocytes	14.3 nTPM
Liver	14.3 nTPM

Collecting duct cells	9.2 nTPM
Kidney	9.2 nTPM

Distal enterocytes	32.0 nTPM
Rectum	36.6 nTPM
Colon	27.5 nTPM

Undifferentiated cells	10.9 nTPM
Rectum	15.7 nTPM
Small intestine	9.5 nTPM
Colon	7.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC