Single cell type - TPM2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TPM2
Gene descriptionⁱ Full gene name according to HGNC.	Tropomyosin 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Smooth muscle cells - ECM organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Smooth muscle cells, Sertoli cells, Peritubular cells, Breast myoepithelial cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (plasmacytoid DC)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (BJ, fHDF/TERT166, hTERT-RPE1)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Smooth muscle cells, Sertoli cells, Peritubular cells, Breast myoepithelial cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TPM2 is part of cluster 48 Smooth muscle cells - ECM organization with confidenceⁱ 1
270 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ACTA2	Actin alpha 2, smooth muscle	0.7660	48
RASL12	RAS like family 12	0.7528	48
KLHL30	Kelch like family member 30	0.7301	37
GPR20	G protein-coupled receptor 20	0.7273	48
TAGLN	Transgelin	0.7246	48
RERGL	RERG like	0.7193	48
MYL9	Myosin light chain 9	0.7175	48
PDLIM3	PDZ and LIM domain 3	0.7161	37
REM1	RRAD and GEM like GTPase 1	0.7115	48
SYNPO2	Synaptopodin 2	0.7066	48
FHL3	Four and a half LIM domains 3	0.7024	37
MYH11	Myosin heavy chain 11	0.6951	48
CCL26	C-C motif chemokine ligand 26	0.6921	48
MRGPRF	MAS related GPR family member F	0.6876	48
IL17B	Interleukin 17B	0.6854	48

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	110.0 nTPM
Breast	193.2 nTPM
Adipose tissue	26.7 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	4.1 nTPM
Lung	4.1 nTPM

Astrocytes	10.4 nTPM
Brain	10.4 nTPM

B-cells	5.9 nTPM
Breast	25.5 nTPM
Kidney	9.5 nTPM
Lymph node	5.8 nTPM
Colon	4.8 nTPM
Liver	4.2 nTPM
Spleen	3.8 nTPM
Stomach	2.7 nTPM
Adipose tissue	2.0 nTPM
Bone marrow	0.8 nTPM
PBMC	0.0 nTPM

Dendritic cells	116.7 nTPM
PBMC	318.8 nTPM
Breast	21.5 nTPM
Adipose tissue	9.7 nTPM

Endothelial cells	80.3 nTPM
Eye	172.3 nTPM
Testis	152.0 nTPM
Breast	146.4 nTPM
Placenta	109.1 nTPM
Heart muscle	95.2 nTPM
Prostate	93.5 nTPM
Skin	90.3 nTPM
Skeletal muscle	88.1 nTPM
Endometrium	47.0 nTPM
Ovary	41.8 nTPM
Liver	28.4 nTPM
Lung	28.1 nTPM
Esophagus	24.2 nTPM
Pancreas	7.7 nTPM

Fibroblasts	332.6 nTPM
Ovary	879.9 nTPM
Placenta	800.3 nTPM
Prostate	467.0 nTPM
Stomach	375.7 nTPM
Breast	290.4 nTPM
Heart muscle	210.9 nTPM
Esophagus	183.6 nTPM
Lung	164.2 nTPM
Skin	107.1 nTPM
Skeletal muscle	96.7 nTPM
Adipose tissue	82.8 nTPM

Intestinal goblet cells	1.9 nTPM
Small intestine	2.4 nTPM
Rectum	1.8 nTPM
Colon	1.4 nTPM

Macrophages	24.2 nTPM
Endometrium	104.6 nTPM
Skeletal muscle	56.3 nTPM
Breast	50.6 nTPM
Prostate	45.8 nTPM
Esophagus	40.9 nTPM
Testis	25.0 nTPM
Ovary	10.9 nTPM
Pancreas	10.2 nTPM
Adipose tissue	6.1 nTPM
Lung	4.3 nTPM
Spleen	3.1 nTPM
Stomach	2.9 nTPM
Bone marrow	1.2 nTPM
Kidney	0.8 nTPM
PBMC	0.0 nTPM

Paneth cells	0.1 nTPM
Rectum	0.2 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	2.9 nTPM
Esophagus	6.1 nTPM
Adipose tissue	5.8 nTPM
Stomach	2.0 nTPM
Spleen	0.6 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	23.5 nTPM
Bronchus	36.7 nTPM
Lung	10.2 nTPM

Smooth muscle cells	2341.4 nTPM
Prostate	5957.3 nTPM
Breast	3763.3 nTPM
Pancreas	3609.4 nTPM
Skin	3403.9 nTPM
Esophagus	2618.9 nTPM
Skeletal muscle	1402.9 nTPM
Adipose tissue	1384.4 nTPM
Endometrium	1112.7 nTPM
Heart muscle	1025.9 nTPM
Ovary	957.0 nTPM
Bronchus	520.1 nTPM

T-cells	16.0 nTPM
Skin	66.3 nTPM
Skeletal muscle	51.5 nTPM
Endometrium	49.4 nTPM
Ovary	37.8 nTPM
Breast	24.2 nTPM
Lung	13.6 nTPM
Esophagus	6.6 nTPM
Colon	3.8 nTPM
Kidney	3.4 nTPM
Liver	3.3 nTPM
Adipose tissue	2.9 nTPM
Stomach	2.4 nTPM
Lymph node	1.9 nTPM
PBMC	1.6 nTPM
Bone marrow	1.5 nTPM
Spleen	1.4 nTPM
Prostate	0.0 nTPM

Basal keratinocytes	57.1 nTPM
Skin	57.1 nTPM

Basal prostatic cells	81.2 nTPM
Prostate	81.2 nTPM

Basal respiratory cells	274.1 nTPM
Bronchus	274.1 nTPM

Basal squamous epithelial cells	24.5 nTPM
Esophagus	24.5 nTPM

Undifferentiated cells	2.8 nTPM
Colon	4.7 nTPM
Small intestine	2.2 nTPM
Rectum	1.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC