Single cell type - IBTK - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	IBTK
Gene descriptionⁱ Full gene name according to HGNC.	Inhibitor of Bruton tyrosine kinase
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Epithelial cell types - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Oligodendrocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Oligodendrocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

IBTK is part of cluster 22 Epithelial cell types - Mixed function with confidenceⁱ 1
298 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
FA2H	Fatty acid 2-hydroxylase	0.5781	22
MYO1E	Myosin IE	0.5506	42
ZNF638	Zinc finger protein 638	0.5102	52
KCNK1	Potassium two pore domain channel subfamily K member 1	0.5081	22
SLC20A2	Solute carrier family 20 member 2	0.5019	22
SMG5	SMG5 nonsense mediated mRNA decay factor	0.4986	53
TJP1	Tight junction protein 1	0.4940	44
LMO7	LIM domain 7	0.4913	22
NPC1	NPC intracellular cholesterol transporter 1	0.4806	42
USP54	Ubiquitin specific peptidase 54	0.4750	42
STRN	Striatin	0.4717	42
SH3RF1	SH3 domain containing ring finger 1	0.4673	22
BAIAP2L1	BAR/IMD domain containing adaptor protein 2 like 1	0.4657	22
ABTB2	Ankyrin repeat and BTB domain containing 2	0.4486	42
JUP	Junction plakoglobin	0.4455	22

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	26.1 nTPM
Adipose tissue	29.4 nTPM
Breast	22.7 nTPM

Alveolar cells type 1	41.7 nTPM
Lung	41.7 nTPM

Alveolar cells type 2	47.6 nTPM
Lung	47.6 nTPM

Astrocytes	46.4 nTPM
Brain	46.4 nTPM

B-cells	33.5 nTPM
Stomach	45.4 nTPM
Bone marrow	44.9 nTPM
Lymph node	39.2 nTPM
PBMC	38.6 nTPM
Spleen	38.4 nTPM
Liver	37.9 nTPM
Kidney	33.4 nTPM
Breast	22.8 nTPM
Colon	17.6 nTPM
Adipose tissue	16.5 nTPM

Dendritic cells	25.9 nTPM
PBMC	35.8 nTPM
Adipose tissue	27.2 nTPM
Breast	14.6 nTPM

Endothelial cells	27.3 nTPM
Heart muscle	52.6 nTPM
Pancreas	34.7 nTPM
Ovary	32.8 nTPM
Endometrium	31.6 nTPM
Skeletal muscle	30.5 nTPM
Prostate	28.2 nTPM
Lung	28.1 nTPM
Testis	26.9 nTPM
Esophagus	25.3 nTPM
Breast	22.7 nTPM
Eye	21.0 nTPM
Skin	21.0 nTPM
Placenta	14.2 nTPM
Liver	11.9 nTPM

Fibroblasts	34.1 nTPM
Stomach	50.5 nTPM
Heart muscle	41.3 nTPM
Skeletal muscle	36.6 nTPM
Adipose tissue	36.2 nTPM
Prostate	36.1 nTPM
Ovary	36.1 nTPM
Esophagus	35.0 nTPM
Breast	29.7 nTPM
Lung	25.7 nTPM
Placenta	25.7 nTPM
Skin	21.8 nTPM

Intestinal goblet cells	49.3 nTPM
Colon	81.4 nTPM
Rectum	41.5 nTPM
Small intestine	24.9 nTPM

Macrophages	36.1 nTPM
Stomach	64.5 nTPM
Spleen	63.2 nTPM
Prostate	45.8 nTPM
Lung	45.1 nTPM
Bone marrow	41.2 nTPM
Ovary	37.6 nTPM
Skeletal muscle	37.1 nTPM
Adipose tissue	36.6 nTPM
Esophagus	36.2 nTPM
Endometrium	33.2 nTPM
Testis	30.3 nTPM
Breast	25.6 nTPM
Kidney	24.2 nTPM
Pancreas	20.4 nTPM
PBMC	0.0 nTPM

Paneth cells	55.5 nTPM
Colon	111.9 nTPM
Rectum	32.1 nTPM
Small intestine	22.5 nTPM

Plasma cells	68.2 nTPM
Spleen	110.2 nTPM
Stomach	105.5 nTPM
Bone marrow	67.6 nTPM
Esophagus	30.7 nTPM
Adipose tissue	27.1 nTPM

Respiratory ciliated cells	42.1 nTPM
Bronchus	46.3 nTPM
Lung	37.9 nTPM

Smooth muscle cells	29.1 nTPM
Esophagus	41.9 nTPM
Adipose tissue	40.3 nTPM
Pancreas	39.3 nTPM
Skeletal muscle	37.8 nTPM
Prostate	28.2 nTPM
Ovary	27.0 nTPM
Breast	26.2 nTPM
Endometrium	26.1 nTPM
Heart muscle	20.2 nTPM
Skin	20.2 nTPM
Bronchus	13.0 nTPM

T-cells	29.8 nTPM
Bone marrow	46.2 nTPM
Stomach	42.4 nTPM
Spleen	38.1 nTPM
Lymph node	35.9 nTPM
Lung	34.9 nTPM
PBMC	34.4 nTPM
Esophagus	32.8 nTPM
Kidney	31.5 nTPM
Ovary	28.9 nTPM
Endometrium	25.8 nTPM
Liver	25.0 nTPM
Skeletal muscle	24.9 nTPM
Adipose tissue	24.6 nTPM
Prostate	23.6 nTPM
Breast	23.2 nTPM
Colon	22.3 nTPM
Skin	11.9 nTPM

Basal keratinocytes	13.6 nTPM
Skin	13.6 nTPM

Basal prostatic cells	42.8 nTPM
Prostate	42.8 nTPM

Basal respiratory cells	19.4 nTPM
Bronchus	19.4 nTPM

Basal squamous epithelial cells	15.4 nTPM
Esophagus	15.4 nTPM

Undifferentiated cells	41.9 nTPM
Colon	62.2 nTPM
Rectum	38.2 nTPM
Small intestine	25.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC