Single cell type - GCLM - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	GCLM
Gene descriptionⁱ Full gene name according to HGNC.	Glutamate-cysteine ligase modifier subunit
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Epithelial cell types - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (Prostatic glandular cells, Urothelial cells, Erythroid cells, Basal prostatic cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Group enriched (Prostatic glandular cells, Urothelial cells, Erythroid cells, Basal prostatic cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

GCLM is part of cluster 22 Epithelial cell types - Mixed function with confidenceⁱ 1
298 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TXNRD1	Thioredoxin reductase 1	0.7955	22
MFSD2B	Major facilitator superfamily domain containing 2B	0.7762	6
ADGRF1	Adhesion G protein-coupled receptor F1	0.7644	22
ARTN	Artemin	0.7446	22
HDGF	Heparin binding growth factor	0.7377	22
LRRC8A	Leucine rich repeat containing 8 VRAC subunit A	0.7287	22
PROSER2	Proline and serine rich 2	0.7187	22
GJB4	Gap junction protein beta 4	0.7141	22
PADI3	Peptidyl arginine deiminase 3	0.7072	22
PLAT	Plasminogen activator, tissue type	0.7034	22
PLEKHN1	Pleckstrin homology domain containing N1	0.6903	22
GAN	Gigaxonin	0.6768	22
CLDN4	Claudin 4	0.6742	22
CYP1B1	Cytochrome P450 family 1 subfamily B member 1	0.6674	22
YRDC	YrdC N6-threonylcarbamoyltransferase domain containing	0.6671	22

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	28.7 nTPM
Adipose tissue	33.0 nTPM
Breast	24.4 nTPM

Alveolar cells type 1	41.7 nTPM
Lung	41.7 nTPM

Alveolar cells type 2	19.1 nTPM
Lung	19.1 nTPM

Astrocytes	16.0 nTPM
Brain	16.0 nTPM

B-cells	14.6 nTPM
Liver	21.5 nTPM
Breast	21.3 nTPM
Colon	20.8 nTPM
Bone marrow	20.4 nTPM
Adipose tissue	15.0 nTPM
Lymph node	14.2 nTPM
Spleen	11.8 nTPM
Kidney	9.5 nTPM
Stomach	7.3 nTPM
PBMC	4.3 nTPM

Dendritic cells	39.3 nTPM
Adipose tissue	55.3 nTPM
Breast	50.6 nTPM
PBMC	12.0 nTPM

Endothelial cells	55.5 nTPM
Prostate	468.9 nTPM
Ovary	46.0 nTPM
Skin	36.0 nTPM
Skeletal muscle	31.8 nTPM
Eye	31.6 nTPM
Liver	31.2 nTPM
Endometrium	27.0 nTPM
Breast	18.3 nTPM
Testis	15.7 nTPM
Lung	15.6 nTPM
Pancreas	15.5 nTPM
Esophagus	15.0 nTPM
Heart muscle	13.7 nTPM
Placenta	11.3 nTPM

Erythroid cells	301.9 nTPM
Liver	534.8 nTPM
Bone marrow	69.0 nTPM

Fibroblasts	44.5 nTPM
Prostate	231.1 nTPM
Skeletal muscle	46.5 nTPM
Placenta	40.9 nTPM
Stomach	40.1 nTPM
Skin	31.8 nTPM
Ovary	26.6 nTPM
Lung	21.2 nTPM
Adipose tissue	18.4 nTPM
Breast	14.1 nTPM
Esophagus	11.2 nTPM
Heart muscle	7.1 nTPM

Intestinal goblet cells	19.9 nTPM
Small intestine	42.3 nTPM
Rectum	10.1 nTPM
Colon	7.2 nTPM

Macrophages	32.0 nTPM
PBMC	163.7 nTPM
Prostate	65.4 nTPM
Skeletal muscle	45.4 nTPM
Pancreas	40.8 nTPM
Adipose tissue	22.6 nTPM
Kidney	21.1 nTPM
Spleen	17.6 nTPM
Breast	14.8 nTPM
Endometrium	14.7 nTPM
Ovary	14.2 nTPM
Testis	12.8 nTPM
Esophagus	12.6 nTPM
Lung	12.3 nTPM
Stomach	11.7 nTPM
Bone marrow	10.0 nTPM

Paneth cells	8.0 nTPM
Rectum	9.8 nTPM
Small intestine	7.5 nTPM
Colon	6.5 nTPM

Plasma cells	21.6 nTPM
Adipose tissue	43.2 nTPM
Bone marrow	28.0 nTPM
Spleen	20.8 nTPM
Stomach	9.7 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	64.3 nTPM
Bronchus	74.6 nTPM
Lung	54.0 nTPM

Smooth muscle cells	37.1 nTPM
Prostate	154.8 nTPM
Pancreas	84.1 nTPM
Skin	37.3 nTPM
Ovary	26.4 nTPM
Bronchus	22.7 nTPM
Skeletal muscle	19.5 nTPM
Adipose tissue	16.1 nTPM
Endometrium	14.4 nTPM
Esophagus	14.0 nTPM
Breast	10.6 nTPM
Heart muscle	8.9 nTPM

Cholangiocytes	61.5 nTPM
Liver	61.5 nTPM

Collecting duct cells	29.5 nTPM
Kidney	29.5 nTPM

Cone photoreceptor cells	6.2 nTPM
Eye	6.2 nTPM

Cytotrophoblasts	63.5 nTPM
Placenta	63.5 nTPM

T-cells	34.1 nTPM
Skin	56.6 nTPM
Adipose tissue	54.4 nTPM
Breast	49.1 nTPM
Prostate	42.6 nTPM
Skeletal muscle	42.3 nTPM
Kidney	41.1 nTPM
Bone marrow	40.6 nTPM
Liver	40.1 nTPM
Spleen	37.8 nTPM
Colon	30.4 nTPM
Endometrium	29.8 nTPM
Ovary	26.7 nTPM
Lymph node	23.8 nTPM
Stomach	23.8 nTPM
PBMC	22.2 nTPM
Lung	12.2 nTPM
Esophagus	6.6 nTPM

Undifferentiated cells	17.8 nTPM
Colon	26.0 nTPM
Small intestine	16.5 nTPM
Rectum	11.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC