Single cell type - RRAGD - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RRAGD
Gene descriptionⁱ Full gene name according to HGNC.	Ras related GTP binding D
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Basic cellular processes (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Cardiomyocytes, Distal tubular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (neutrophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Karpas-707, SCLC-21H, U-266/70, U-266/84)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Cardiomyocytes, Distal tubular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RRAGD is part of cluster 39 Non-specific - Basic cellular processes with confidenceⁱ 1
586 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SSC4D	Scavenger receptor cysteine rich family member with 4 domains	0.5486	7
ATP6V1F	ATPase H+ transporting V1 subunit F	0.5295	4
MRAS	Muscle RAS oncogene homolog	0.5291	54
PFKM	Phosphofructokinase, muscle	0.5010	54
PRKAA2	Protein kinase AMP-activated catalytic subunit alpha 2	0.5003	54
KCNJ11	Potassium inwardly rectifying channel subfamily J member 11	0.4955	54
MPC1	Mitochondrial pyruvate carrier 1	0.4763	39
FSD2	Fibronectin type III and SPRY domain containing 2	0.4728	54
FABP3	Fatty acid binding protein 3	0.4706	30
PLA2G15	Phospholipase A2 group XV	0.4651	4
TMEM126A	Transmembrane protein 126A	0.4639	39
DUSP3	Dual specificity phosphatase 3	0.4567	54
TMEM182	Transmembrane protein 182	0.4546	54
UQCR10	Ubiquinol-cytochrome c reductase, complex III subunit X	0.4510	39
ANKRD2	Ankyrin repeat domain 2	0.4494	54

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.4 nTPM
Breast	2.4 nTPM
Adipose tissue	0.5 nTPM

Alveolar cells type 1	9.6 nTPM
Lung	9.6 nTPM

Alveolar cells type 2	16.7 nTPM
Lung	16.7 nTPM

Astrocytes	5.2 nTPM
Brain	5.2 nTPM

B-cells	3.7 nTPM
Liver	10.1 nTPM
Breast	8.1 nTPM
Adipose tissue	7.0 nTPM
Colon	4.8 nTPM
PBMC	2.8 nTPM
Bone marrow	1.7 nTPM
Spleen	1.7 nTPM
Lymph node	0.7 nTPM
Stomach	0.5 nTPM
Kidney	0.0 nTPM

Dendritic cells	4.7 nTPM
PBMC	12.0 nTPM
Adipose tissue	1.7 nTPM
Breast	0.4 nTPM

Endothelial cells	4.8 nTPM
Heart muscle	20.1 nTPM
Prostate	10.8 nTPM
Lung	9.3 nTPM
Breast	4.9 nTPM
Skin	4.5 nTPM
Pancreas	3.9 nTPM
Liver	2.7 nTPM
Eye	2.4 nTPM
Skeletal muscle	2.2 nTPM
Esophagus	2.1 nTPM
Placenta	1.7 nTPM
Testis	1.4 nTPM
Endometrium	0.9 nTPM
Ovary	0.5 nTPM

Fibroblasts	10.0 nTPM
Heart muscle	47.0 nTPM
Prostate	13.9 nTPM
Skin	13.1 nTPM
Esophagus	11.0 nTPM
Skeletal muscle	6.1 nTPM
Adipose tissue	5.8 nTPM
Lung	4.5 nTPM
Breast	4.0 nTPM
Stomach	3.0 nTPM
Placenta	1.2 nTPM
Ovary	0.8 nTPM

Intestinal goblet cells	0.5 nTPM
Small intestine	1.1 nTPM
Rectum	0.4 nTPM
Colon	0.0 nTPM

Macrophages	23.2 nTPM
Ovary	96.5 nTPM
Prostate	52.7 nTPM
Lung	40.6 nTPM
Pancreas	28.0 nTPM
Spleen	19.7 nTPM
Skeletal muscle	16.1 nTPM
Adipose tissue	15.9 nTPM
Endometrium	14.7 nTPM
PBMC	13.6 nTPM
Bone marrow	12.5 nTPM
Stomach	11.7 nTPM
Kidney	8.3 nTPM
Breast	7.2 nTPM
Esophagus	6.3 nTPM
Testis	4.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	8.0 nTPM
Esophagus	12.3 nTPM
Spleen	9.4 nTPM
Adipose tissue	7.8 nTPM
Stomach	5.5 nTPM
Bone marrow	5.0 nTPM

Respiratory ciliated cells	19.4 nTPM
Bronchus	24.1 nTPM
Lung	14.6 nTPM

Smooth muscle cells	7.0 nTPM
Heart muscle	17.5 nTPM
Bronchus	8.4 nTPM
Prostate	7.5 nTPM
Skin	6.4 nTPM
Adipose tissue	6.4 nTPM
Esophagus	6.2 nTPM
Skeletal muscle	6.0 nTPM
Endometrium	5.6 nTPM
Breast	4.9 nTPM
Pancreas	4.3 nTPM
Ovary	3.4 nTPM

T-cells	3.9 nTPM
Prostate	37.9 nTPM
Adipose tissue	7.0 nTPM
Kidney	3.4 nTPM
Skeletal muscle	3.3 nTPM
Skin	2.8 nTPM
Breast	2.0 nTPM
Spleen	1.7 nTPM
Lung	1.5 nTPM
PBMC	1.3 nTPM
Endometrium	1.3 nTPM
Lymph node	1.3 nTPM
Liver	1.1 nTPM
Bone marrow	1.1 nTPM
Stomach	0.6 nTPM
Colon	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	16.2 nTPM
Skin	16.2 nTPM

Basal prostatic cells	17.0 nTPM
Prostate	17.0 nTPM

Cholangiocytes	17.2 nTPM
Liver	17.2 nTPM

Cone photoreceptor cells	31.3 nTPM
Eye	31.3 nTPM

Undifferentiated cells	0.2 nTPM
Rectum	0.3 nTPM
Small intestine	0.2 nTPM
Colon	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC