Single cell type - TRAM2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TRAM2
Gene descriptionⁱ Full gene name according to HGNC.	Translocation associated membrane protein 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Plasma cells - Humoral immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TRAM2 is part of cluster 55 Plasma cells - Humoral immune response with confidenceⁱ 1
379 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PRDX4	Peroxiredoxin 4	0.6195	55
SLC38A10	Solute carrier family 38 member 10	0.5803	55
SSR4	Signal sequence receptor subunit 4	0.5238	55
TXNDC15	Thioredoxin domain containing 15	0.5219	55
FNDC3B	Fibronectin type III domain containing 3B	0.4996	55
IGLV5-48	Immunoglobulin lambda variable 5-48 (non-functional)	0.4883	55
RABAC1	Rab acceptor 1	0.4727	25
IKBIP	IKBKB interacting protein	0.4719	33
BET1L	Bet1 golgi vesicular membrane trafficking protein like	0.4713	31
SEC24D	SEC24 homolog D, COPII coat complex component	0.4659	55
UBE2J1	Ubiquitin conjugating enzyme E2 J1	0.4642	55
SEC31A	SEC31 homolog A, COPII coat complex component	0.4600	40
FKBP11	FKBP prolyl isomerase 11	0.4550	55
MARVELD1	MARVEL domain containing 1	0.4537	40
DERL3	Derlin 3	0.4516	55

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	12.8 nTPM
Adipose tissue	13.6 nTPM
Breast	12.0 nTPM

Alveolar cells type 1	6.4 nTPM
Lung	6.4 nTPM

Alveolar cells type 2	3.2 nTPM
Lung	3.2 nTPM

Astrocytes	6.8 nTPM
Brain	6.8 nTPM

B-cells	15.9 nTPM
Liver	73.2 nTPM
Breast	20.0 nTPM
Stomach	17.2 nTPM
Adipose tissue	12.0 nTPM
Kidney	9.5 nTPM
Colon	8.0 nTPM
Lymph node	7.2 nTPM
Spleen	4.9 nTPM
PBMC	4.3 nTPM
Bone marrow	3.2 nTPM

Dendritic cells	6.6 nTPM
PBMC	8.0 nTPM
Adipose tissue	7.3 nTPM
Breast	4.7 nTPM

Endothelial cells	17.9 nTPM
Liver	36.7 nTPM
Ovary	31.9 nTPM
Endometrium	26.8 nTPM
Heart muscle	19.0 nTPM
Skeletal muscle	19.0 nTPM
Placenta	18.8 nTPM
Eye	17.8 nTPM
Prostate	15.3 nTPM
Skin	13.9 nTPM
Esophagus	12.9 nTPM
Pancreas	11.6 nTPM
Testis	10.6 nTPM
Breast	10.6 nTPM
Lung	6.2 nTPM

Fibroblasts	26.2 nTPM
Stomach	38.6 nTPM
Placenta	36.2 nTPM
Adipose tissue	36.2 nTPM
Skin	32.0 nTPM
Skeletal muscle	29.5 nTPM
Heart muscle	25.2 nTPM
Breast	24.3 nTPM
Esophagus	21.8 nTPM
Ovary	21.3 nTPM
Prostate	13.3 nTPM
Lung	10.1 nTPM

Intestinal goblet cells	19.6 nTPM
Small intestine	25.1 nTPM
Rectum	18.8 nTPM
Colon	15.0 nTPM

Macrophages	5.7 nTPM
Skeletal muscle	10.5 nTPM
Ovary	10.5 nTPM
Kidney	8.3 nTPM
Endometrium	7.9 nTPM
Spleen	7.2 nTPM
Bone marrow	6.2 nTPM
Lung	5.9 nTPM
Stomach	5.9 nTPM
Adipose tissue	5.3 nTPM
Esophagus	4.8 nTPM
Prostate	4.6 nTPM
Testis	4.6 nTPM
Breast	3.5 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM

Paneth cells	1.7 nTPM
Rectum	5.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	69.5 nTPM
Stomach	107.7 nTPM
Esophagus	86.2 nTPM
Bone marrow	70.9 nTPM
Spleen	70.6 nTPM
Adipose tissue	12.2 nTPM

Respiratory ciliated cells	4.9 nTPM
Lung	5.8 nTPM
Bronchus	3.9 nTPM

Smooth muscle cells	15.7 nTPM
Pancreas	33.1 nTPM
Ovary	17.2 nTPM
Endometrium	17.1 nTPM
Adipose tissue	15.4 nTPM
Esophagus	14.0 nTPM
Skeletal muscle	13.5 nTPM
Prostate	13.4 nTPM
Breast	13.3 nTPM
Skin	13.2 nTPM
Heart muscle	11.6 nTPM
Bronchus	10.5 nTPM

T-cells	6.0 nTPM
Prostate	14.2 nTPM
Skeletal muscle	11.4 nTPM
Skin	9.7 nTPM
PBMC	7.8 nTPM
Endometrium	7.8 nTPM
Kidney	6.9 nTPM
Lung	6.1 nTPM
Stomach	4.8 nTPM
Colon	4.7 nTPM
Breast	4.7 nTPM
Adipose tissue	4.6 nTPM
Liver	4.5 nTPM
Bone marrow	4.5 nTPM
Spleen	4.2 nTPM
Lymph node	3.0 nTPM
Ovary	2.2 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	8.2 nTPM
Skin	8.2 nTPM

Basal prostatic cells	2.8 nTPM
Prostate	2.8 nTPM

Basal respiratory cells	8.5 nTPM
Bronchus	8.5 nTPM

Basal squamous epithelial cells	3.4 nTPM
Esophagus	3.4 nTPM

Undifferentiated cells	5.7 nTPM
Colon	7.2 nTPM
Rectum	6.0 nTPM
Small intestine	3.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC