Single cell type - NDUFB4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NDUFB4
Gene descriptionⁱ Full gene name according to HGNC.	NADH:ubiquinone oxidoreductase subunit B4
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Basic cellular processes (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Cardiomyocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Cardiomyocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NDUFB4 is part of cluster 39 Non-specific - Basic cellular processes with confidenceⁱ 1
586 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
NDUFB3	NADH:ubiquinone oxidoreductase subunit B3	0.7358	39
NDUFC1	NADH:ubiquinone oxidoreductase subunit C1	0.7235	39
UQCR10	Ubiquinol-cytochrome c reductase, complex III subunit X	0.7090	39
NDUFA4	NDUFA4 mitochondrial complex associated	0.6978	39
NDUFA1	NADH:ubiquinone oxidoreductase subunit A1	0.6810	39
SLIRP	SRA stem-loop interacting RNA binding protein	0.6609	39
NDUFB2	NADH:ubiquinone oxidoreductase subunit B2	0.6554	39
TNNI3K	TNNI3 interacting kinase	0.6552	54
UQCRFS1	Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1	0.6552	39
COX7A2	Cytochrome c oxidase subunit 7A2	0.6520	39
UQCRB	Ubiquinol-cytochrome c reductase binding protein	0.6495	39
MDH2	Malate dehydrogenase 2	0.6446	39
COX7B	Cytochrome c oxidase subunit 7B	0.6391	39
COX6B1	Cytochrome c oxidase subunit 6B1	0.6213	39
PDHA1	Pyruvate dehydrogenase E1 subunit alpha 1	0.6192	39

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	274.1 nTPM
Breast	316.2 nTPM
Adipose tissue	232.1 nTPM

Alveolar cells type 1	163.5 nTPM
Lung	163.5 nTPM

Alveolar cells type 2	162.6 nTPM
Lung	162.6 nTPM

Astrocytes	36.9 nTPM
Brain	36.9 nTPM

B-cells	167.0 nTPM
Liver	450.7 nTPM
Breast	196.7 nTPM
PBMC	174.1 nTPM
Kidney	166.7 nTPM
Colon	148.6 nTPM
Spleen	126.0 nTPM
Adipose tissue	122.8 nTPM
Stomach	113.5 nTPM
Bone marrow	87.4 nTPM
Lymph node	83.1 nTPM

Basal squamous epithelial cells	190.6 nTPM
Esophagus	190.6 nTPM

Dendritic cells	158.1 nTPM
PBMC	215.2 nTPM
Adipose tissue	135.0 nTPM
Breast	124.3 nTPM

Endothelial cells	235.7 nTPM
Pancreas	359.0 nTPM
Testis	355.0 nTPM
Placenta	278.0 nTPM
Prostate	268.1 nTPM
Heart muscle	266.7 nTPM
Breast	245.2 nTPM
Liver	244.0 nTPM
Skin	225.1 nTPM
Ovary	203.1 nTPM
Skeletal muscle	201.0 nTPM
Endometrium	182.3 nTPM
Eye	175.6 nTPM
Esophagus	159.7 nTPM
Lung	137.3 nTPM

Enteroendocrine cells	298.7 nTPM
Colon	330.4 nTPM
Rectum	266.9 nTPM

Erythroid cells	196.9 nTPM
Liver	229.9 nTPM
Bone marrow	163.9 nTPM

Fibroblasts	213.7 nTPM
Heart muscle	449.5 nTPM
Placenta	267.0 nTPM
Breast	235.8 nTPM
Adipose tissue	218.5 nTPM
Prostate	202.2 nTPM
Lung	192.1 nTPM
Skin	189.7 nTPM
Esophagus	167.0 nTPM
Ovary	151.7 nTPM
Skeletal muscle	140.7 nTPM
Stomach	136.6 nTPM

Intestinal goblet cells	526.3 nTPM
Colon	747.6 nTPM
Rectum	494.1 nTPM
Small intestine	337.3 nTPM

Macrophages	196.2 nTPM
Pancreas	359.9 nTPM
Kidney	336.6 nTPM
PBMC	286.4 nTPM
Testis	275.3 nTPM
Adipose tissue	207.5 nTPM
Ovary	202.0 nTPM
Prostate	192.6 nTPM
Breast	183.1 nTPM
Endometrium	179.0 nTPM
Lung	160.8 nTPM
Bone marrow	131.2 nTPM
Esophagus	125.9 nTPM
Skeletal muscle	125.6 nTPM
Spleen	98.5 nTPM
Stomach	79.1 nTPM

Paneth cells	581.8 nTPM
Colon	876.2 nTPM
Rectum	482.6 nTPM
Small intestine	386.7 nTPM

Plasma cells	283.2 nTPM
Spleen	365.7 nTPM
Stomach	330.8 nTPM
Esophagus	314.0 nTPM
Bone marrow	275.2 nTPM
Adipose tissue	130.3 nTPM

Cholangiocytes	311.7 nTPM
Liver	311.7 nTPM

Collecting duct cells	320.0 nTPM
Kidney	320.0 nTPM

Cone photoreceptor cells	58.4 nTPM
Eye	58.4 nTPM

Cytotrophoblasts	366.0 nTPM
Placenta	366.0 nTPM

Respiratory ciliated cells	266.2 nTPM
Bronchus	304.9 nTPM
Lung	227.4 nTPM

Smooth muscle cells	259.0 nTPM
Heart muscle	396.4 nTPM
Pancreas	370.2 nTPM
Breast	291.6 nTPM
Prostate	276.0 nTPM
Skeletal muscle	272.9 nTPM
Skin	269.9 nTPM
Adipose tissue	260.9 nTPM
Esophagus	200.0 nTPM
Ovary	189.4 nTPM
Endometrium	171.2 nTPM
Bronchus	150.2 nTPM

T-cells	168.1 nTPM
Kidney	290.9 nTPM
Skin	261.6 nTPM
PBMC	219.2 nTPM
Liver	201.1 nTPM
Adipose tissue	184.1 nTPM
Prostate	179.9 nTPM
Colon	172.0 nTPM
Lung	167.0 nTPM
Breast	159.7 nTPM
Spleen	154.1 nTPM
Skeletal muscle	150.5 nTPM
Endometrium	144.9 nTPM
Stomach	132.5 nTPM
Bone marrow	119.9 nTPM
Lymph node	111.6 nTPM
Esophagus	104.8 nTPM
Ovary	104.5 nTPM

Undifferentiated cells	462.8 nTPM
Colon	546.6 nTPM
Rectum	451.6 nTPM
Small intestine	390.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC