Single cell type - PPP2R5A - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PPP2R5A
Gene descriptionⁱ Full gene name according to HGNC.	Protein phosphatase 2 regulatory subunit B'alpha
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PPP2R5A is part of cluster 25 Spermatids - Spermatogenesis with confidenceⁱ 1
458 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
METAP1	Methionyl aminopeptidase 1	0.7508	25
TEX38	Testis expressed 38	0.7297	25
UBQLN3	Ubiquilin 3	0.7150	25
UBL4B	Ubiquitin like 4B	0.7121	25
THEG	Theg spermatid protein	0.7036	25
NUP35	Nucleoporin 35	0.7032	25
SMCP	Sperm mitochondria associated cysteine rich protein	0.7010	25
NUPR2	Nuclear protein 2, transcriptional regulator	0.6999	25
DGCR6L	DiGeorge syndrome critical region gene 6 like	0.6946	25
LELP1	Late cornified envelope like proline rich 1	0.6909	25
DCUN1D1	Defective in cullin neddylation 1 domain containing 1	0.6903	25
PROCA1	Protein interacting with cyclin A1	0.6897	25
KLHL10	Kelch like family member 10	0.6892	25
FSCB	Fibrous sheath CABYR binding protein	0.6887	25
BOD1L2	Biorientation of chromosomes in cell division 1 like 2	0.6867	25

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	66.7 nTPM
Adipose tissue	71.9 nTPM
Breast	61.5 nTPM

Alveolar cells type 1	99.3 nTPM
Lung	99.3 nTPM

Alveolar cells type 2	104.4 nTPM
Lung	104.4 nTPM

Astrocytes	71.5 nTPM
Brain	71.5 nTPM

B-cells	34.2 nTPM
Lymph node	60.1 nTPM
Kidney	47.6 nTPM
Stomach	46.4 nTPM
Bone marrow	44.2 nTPM
Spleen	41.5 nTPM
Adipose tissue	34.1 nTPM
PBMC	20.0 nTPM
Liver	18.0 nTPM
Colon	16.0 nTPM
Breast	14.7 nTPM

Dendritic cells	56.2 nTPM
Adipose tissue	95.6 nTPM
Breast	60.9 nTPM
PBMC	12.0 nTPM

Endothelial cells	53.3 nTPM
Endometrium	101.8 nTPM
Esophagus	92.4 nTPM
Testis	67.3 nTPM
Liver	54.1 nTPM
Skin	54.0 nTPM
Breast	53.7 nTPM
Skeletal muscle	53.5 nTPM
Heart muscle	52.3 nTPM
Placenta	52.3 nTPM
Ovary	50.2 nTPM
Prostate	37.3 nTPM
Lung	31.2 nTPM
Eye	26.7 nTPM
Pancreas	19.3 nTPM

Fibroblasts	18.7 nTPM
Skin	26.2 nTPM
Esophagus	24.0 nTPM
Stomach	23.7 nTPM
Heart muscle	21.5 nTPM
Prostate	18.4 nTPM
Skeletal muscle	18.3 nTPM
Adipose tissue	18.2 nTPM
Breast	16.6 nTPM
Placenta	14.7 nTPM
Ovary	14.2 nTPM
Lung	10.1 nTPM

Intestinal goblet cells	33.9 nTPM
Small intestine	47.7 nTPM
Rectum	35.3 nTPM
Colon	18.6 nTPM

Macrophages	34.1 nTPM
Bone marrow	60.0 nTPM
Spleen	50.8 nTPM
Ovary	45.5 nTPM
Stomach	43.9 nTPM
PBMC	40.9 nTPM
Adipose tissue	33.7 nTPM
Lung	33.3 nTPM
Skeletal muscle	31.6 nTPM
Esophagus	28.3 nTPM
Pancreas	28.0 nTPM
Endometrium	27.0 nTPM
Kidney	26.5 nTPM
Prostate	25.3 nTPM
Breast	23.8 nTPM
Testis	12.8 nTPM

Paneth cells	32.5 nTPM
Rectum	44.0 nTPM
Small intestine	33.8 nTPM
Colon	19.7 nTPM

Plasma cells	29.6 nTPM
Adipose tissue	56.8 nTPM
Spleen	26.1 nTPM
Esophagus	24.6 nTPM
Stomach	22.6 nTPM
Bone marrow	18.2 nTPM

Respiratory ciliated cells	21.3 nTPM
Bronchus	22.2 nTPM
Lung	20.4 nTPM

Smooth muscle cells	36.5 nTPM
Esophagus	83.7 nTPM
Breast	58.7 nTPM
Heart muscle	50.0 nTPM
Skin	41.2 nTPM
Adipose tissue	41.0 nTPM
Skeletal muscle	40.6 nTPM
Prostate	40.0 nTPM
Endometrium	18.8 nTPM
Pancreas	10.7 nTPM
Ovary	9.8 nTPM
Bronchus	6.6 nTPM

T-cells	54.1 nTPM
Adipose tissue	100.4 nTPM
Prostate	71.1 nTPM
Spleen	70.7 nTPM
Lymph node	69.8 nTPM
Bone marrow	59.0 nTPM
Esophagus	58.9 nTPM
Stomach	58.5 nTPM
Skeletal muscle	56.9 nTPM
Ovary	55.6 nTPM
Breast	53.6 nTPM
Kidney	52.0 nTPM
Endometrium	43.4 nTPM
Colon	43.2 nTPM
Liver	37.8 nTPM
PBMC	33.5 nTPM
Skin	31.3 nTPM
Lung	24.3 nTPM

Basal keratinocytes	68.6 nTPM
Skin	68.6 nTPM

Basal prostatic cells	22.1 nTPM
Prostate	22.1 nTPM

Basal respiratory cells	9.0 nTPM
Bronchus	9.0 nTPM

Cholangiocytes	47.1 nTPM
Liver	47.1 nTPM

Undifferentiated cells	30.8 nTPM
Rectum	36.3 nTPM
Small intestine	33.0 nTPM
Colon	23.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC