Single cell type - ASPM - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ASPM
Gene descriptionⁱ Full gene name according to HGNC.	Assembly factor for spindle microtubules
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Cell cycle regulation (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Granulosa cells, Erythroid cells, Plasma cells, Spermatogonia, Spermatocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Granulosa cells, Erythroid cells, Plasma cells, Spermatogonia, Spermatocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ASPM is part of cluster 12 Non-specific - Cell cycle regulation with confidenceⁱ 1
294 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CENPF	Centromere protein F	0.9132	12
MXD3	MAX dimerization protein 3	0.8886	12
ARHGAP11A	Rho GTPase activating protein 11A	0.8785	12
KIF14	Kinesin family member 14	0.8769	12
NEIL3	Nei like DNA glycosylase 3	0.8769	12
BUB1	BUB1 mitotic checkpoint serine/threonine kinase	0.8684	12
MKI67	Marker of proliferation Ki-67	0.8665	12
CENPE	Centromere protein E	0.8558	12
KIF4A	Kinesin family member 4A	0.8548	12
KIF18B	Kinesin family member 18B	0.8510	12
KIF11	Kinesin family member 11	0.8485	12
TPX2	TPX2 microtubule nucleation factor	0.8453	12
GTSE1	G2 and S-phase expressed 1	0.8418	12
CENPA	Centromere protein A	0.8413	12
NUSAP1	Nucleolar and spindle associated protein 1	0.8372	12

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	0.6 nTPM
Breast	1.3 nTPM
Adipose tissue	0.0 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	1.1 nTPM
Lung	1.1 nTPM

Astrocytes	1.3 nTPM
Brain	1.3 nTPM

B-cells	5.8 nTPM
Liver	42.2 nTPM
Colon	8.0 nTPM
Breast	4.0 nTPM
Lymph node	1.5 nTPM
Spleen	1.4 nTPM
Adipose tissue	1.0 nTPM
Stomach	0.5 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Bone marrow	0.0 nTPM

Dendritic cells	7.8 nTPM
Adipose tissue	11.9 nTPM
Breast	7.6 nTPM
PBMC	4.0 nTPM

Endothelial cells	4.3 nTPM
Ovary	23.7 nTPM
Endometrium	19.7 nTPM
Placenta	12.5 nTPM
Testis	0.9 nTPM
Breast	0.8 nTPM
Heart muscle	0.7 nTPM
Skin	0.7 nTPM
Skeletal muscle	0.5 nTPM
Prostate	0.4 nTPM
Eye	0.0 nTPM
Liver	0.0 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM
Esophagus	0.0 nTPM

Fibroblasts	9.8 nTPM
Ovary	82.4 nTPM
Placenta	10.8 nTPM
Lung	10.1 nTPM
Breast	2.1 nTPM
Skin	1.4 nTPM
Esophagus	0.6 nTPM
Heart muscle	0.3 nTPM
Skeletal muscle	0.3 nTPM
Prostate	0.2 nTPM
Adipose tissue	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	6.8 nTPM
Colon	10.0 nTPM
Small intestine	8.9 nTPM
Rectum	1.4 nTPM

Macrophages	6.4 nTPM
Esophagus	31.5 nTPM
Endometrium	22.9 nTPM
Pancreas	7.7 nTPM
Testis	6.9 nTPM
Breast	5.5 nTPM
Bone marrow	5.0 nTPM
Lung	3.8 nTPM
Prostate	3.4 nTPM
Adipose tissue	3.2 nTPM
Skeletal muscle	2.3 nTPM
Ovary	2.3 nTPM
Spleen	2.1 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	0.2 nTPM
Rectum	0.6 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	44.8 nTPM
Spleen	111.8 nTPM
Bone marrow	90.6 nTPM
Adipose tissue	9.7 nTPM
Esophagus	6.1 nTPM
Stomach	6.0 nTPM

Respiratory ciliated cells	0.8 nTPM
Bronchus	1.5 nTPM
Lung	0.0 nTPM

Smooth muscle cells	7.0 nTPM
Endometrium	32.1 nTPM
Ovary	24.4 nTPM
Pancreas	16.6 nTPM
Bronchus	1.9 nTPM
Heart muscle	1.3 nTPM
Breast	0.8 nTPM
Skeletal muscle	0.2 nTPM
Prostate	0.0 nTPM
Skin	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	9.1 nTPM
Esophagus	32.8 nTPM
Ovary	20.1 nTPM
Colon	13.3 nTPM
Liver	13.3 nTPM
Endometrium	13.0 nTPM
Skeletal muscle	10.2 nTPM
Adipose tissue	9.9 nTPM
Lung	9.1 nTPM
Lymph node	8.4 nTPM
Skin	6.0 nTPM
Bone marrow	4.9 nTPM
Kidney	4.2 nTPM
Spleen	3.6 nTPM
Breast	3.5 nTPM
PBMC	2.4 nTPM
Stomach	0.6 nTPM
Prostate	0.0 nTPM

Basal squamous epithelial cells	2.2 nTPM
Esophagus	2.2 nTPM

Collecting duct cells	1.8 nTPM
Kidney	1.8 nTPM

Cytotrophoblasts	20.0 nTPM
Placenta	20.0 nTPM

Early spermatids	3.9 nTPM
Testis	3.9 nTPM

Undifferentiated cells	16.9 nTPM
Small intestine	20.6 nTPM
Colon	19.7 nTPM
Rectum	10.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC