Single cell type - FSTL3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	FSTL3
Gene descriptionⁱ Full gene name according to HGNC.	Follistatin like 3
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Extravillous trophoblasts - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enriched (Extravillous trophoblasts)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (BEWO, hTCEpi, SiHa, U-2197)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enriched (Extravillous trophoblasts)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

FSTL3 is part of cluster 38 Extravillous trophoblasts - Unknown function with confidenceⁱ 1
176 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HTRA4	HtrA serine peptidase 4	0.9728	38
NLRP9	NLR family pyrin domain containing 9	0.9674	38
NOTUM	Notum, palmitoleoyl-protein carboxylesterase	0.9659	38
HLA-G	Major histocompatibility complex, class I, G	0.9589	38
CLDN19	Claudin 19	0.9510	38
CCNE1	Cyclin E1	0.9498	38
C1QTNF6	C1q and TNF related 6	0.9407	38
OXGR1	Oxoglutarate receptor 1	0.9401	38
KISS1R	KISS1 receptor	0.9350	38
GPR32	G protein-coupled receptor 32	0.9348	38
PRG2	Proteoglycan 2, pro eosinophil major basic protein	0.9336	38
HAPLN3	Hyaluronan and proteoglycan link protein 3	0.9323	38
TLX3	T cell leukemia homeobox 3	0.9283	38
ASCL2	Achaete-scute family bHLH transcription factor 2	0.9247	38
SEPTIN3	Septin 3	0.9166	38

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	4.6 nTPM
Adipose tissue	5.1 nTPM
Breast	4.0 nTPM

Alveolar cells type 1	19.2 nTPM
Lung	19.2 nTPM

Alveolar cells type 2	1.9 nTPM
Lung	1.9 nTPM

Astrocytes	40.5 nTPM
Brain	40.5 nTPM

B-cells	1.5 nTPM
Kidney	4.8 nTPM
PBMC	2.8 nTPM
Liver	2.1 nTPM
Colon	1.6 nTPM
Stomach	0.9 nTPM
Bone marrow	0.8 nTPM
Lymph node	0.6 nTPM
Adipose tissue	0.5 nTPM
Spleen	0.3 nTPM
Breast	0.0 nTPM

Dendritic cells	1.0 nTPM
Breast	1.8 nTPM
Adipose tissue	1.1 nTPM
PBMC	0.0 nTPM

Endothelial cells	8.7 nTPM
Placenta	37.5 nTPM
Prostate	20.5 nTPM
Lung	15.6 nTPM
Skin	7.3 nTPM
Liver	6.4 nTPM
Breast	5.9 nTPM
Endometrium	5.7 nTPM
Skeletal muscle	4.4 nTPM
Pancreas	3.9 nTPM
Ovary	3.7 nTPM
Heart muscle	3.5 nTPM
Testis	2.7 nTPM
Eye	2.4 nTPM
Esophagus	2.1 nTPM

Fibroblasts	59.6 nTPM
Placenta	147.9 nTPM
Prostate	143.1 nTPM
Stomach	75.7 nTPM
Adipose tissue	73.5 nTPM
Skeletal muscle	65.5 nTPM
Skin	40.6 nTPM
Breast	36.5 nTPM
Esophagus	28.0 nTPM
Heart muscle	24.2 nTPM
Lung	13.4 nTPM
Ovary	7.6 nTPM

Intestinal goblet cells	1.0 nTPM
Rectum	1.2 nTPM
Small intestine	1.1 nTPM
Colon	0.7 nTPM

Macrophages	7.4 nTPM
Prostate	17.2 nTPM
Adipose tissue	16.7 nTPM
Pancreas	15.3 nTPM
Endometrium	10.3 nTPM
Ovary	10.3 nTPM
Kidney	9.1 nTPM
Skeletal muscle	7.0 nTPM
Breast	5.6 nTPM
Esophagus	4.8 nTPM
Spleen	4.2 nTPM
Testis	3.5 nTPM
Stomach	2.9 nTPM
Bone marrow	2.5 nTPM
Lung	1.5 nTPM
PBMC	0.0 nTPM

Paneth cells	0.2 nTPM
Rectum	0.6 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	1.0 nTPM
Adipose tissue	3.3 nTPM
Stomach	1.4 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	3.1 nTPM
Bronchus	3.2 nTPM
Lung	2.9 nTPM

Smooth muscle cells	57.0 nTPM
Prostate	231.3 nTPM
Pancreas	128.4 nTPM
Ovary	68.2 nTPM
Skeletal muscle	42.9 nTPM
Esophagus	35.7 nTPM
Adipose tissue	35.3 nTPM
Skin	34.2 nTPM
Breast	23.4 nTPM
Endometrium	16.7 nTPM
Heart muscle	7.3 nTPM
Bronchus	3.8 nTPM

T-cells	2.0 nTPM
Prostate	4.7 nTPM
Ovary	4.5 nTPM
Skin	3.6 nTPM
Kidney	3.4 nTPM
Endometrium	3.4 nTPM
Lung	3.0 nTPM
Liver	2.4 nTPM
Breast	2.3 nTPM
PBMC	1.8 nTPM
Skeletal muscle	1.8 nTPM
Adipose tissue	0.8 nTPM
Bone marrow	0.8 nTPM
Colon	0.5 nTPM
Spleen	0.5 nTPM
Lymph node	0.3 nTPM
Stomach	0.2 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	14.5 nTPM
Skin	14.5 nTPM

Basal prostatic cells	31.0 nTPM
Prostate	31.0 nTPM

Basal respiratory cells	11.7 nTPM
Bronchus	11.7 nTPM

Cholangiocytes	32.9 nTPM
Liver	32.9 nTPM

Undifferentiated cells	2.5 nTPM
Colon	4.3 nTPM
Rectum	1.7 nTPM
Small intestine	1.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC