Single cell type - ASCL2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ASCL2
Gene descriptionⁱ Full gene name according to HGNC.	Achaete-scute family bHLH transcription factor 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Transcription factors
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Extravillous trophoblasts - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enriched (Extravillous trophoblasts)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (eosinophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Group enriched (BEWO, NB-4, OE19, SCLC-21H)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enriched (Extravillous trophoblasts)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ASCL2 is part of cluster 38 Extravillous trophoblasts - Unknown function with confidenceⁱ 1
176 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
NLRP9	NLR family pyrin domain containing 9	0.9676	38
HLA-G	Major histocompatibility complex, class I, G	0.9672	38
OXGR1	Oxoglutarate receptor 1	0.9655	38
NOTUM	Notum, palmitoleoyl-protein carboxylesterase	0.9617	38
HTRA4	HtrA serine peptidase 4	0.9614	38
PRG2	Proteoglycan 2, pro eosinophil major basic protein	0.9580	38
CCNE1	Cyclin E1	0.9538	38
CLDN19	Claudin 19	0.9442	38
HAPLN3	Hyaluronan and proteoglycan link protein 3	0.9361	38
FSTL3	Follistatin like 3	0.9247	38
DLX4	Distal-less homeobox 4	0.9233	38
LAIR2	Leukocyte associated immunoglobulin like receptor 2	0.9215	38
KISS1R	KISS1 receptor	0.9194	38
C1QTNF6	C1q and TNF related 6	0.9180	38
TLX3	T cell leukemia homeobox 3	0.9165	38

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.2 nTPM
Breast	1.8 nTPM
Adipose tissue	0.7 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	0.0 nTPM
Lung	0.0 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	1.4 nTPM
Colon	4.8 nTPM
PBMC	4.3 nTPM
Liver	3.3 nTPM
Adipose tissue	1.0 nTPM
Stomach	0.5 nTPM
Kidney	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Breast	0.0 nTPM

Dendritic cells	29.2 nTPM
Breast	57.3 nTPM
Adipose tissue	30.2 nTPM
PBMC	0.0 nTPM

Endothelial cells	2.6 nTPM
Liver	17.4 nTPM
Eye	7.3 nTPM
Placenta	4.0 nTPM
Breast	2.9 nTPM
Skin	1.3 nTPM
Prostate	1.1 nTPM
Testis	1.1 nTPM
Ovary	0.6 nTPM
Heart muscle	0.4 nTPM
Endometrium	0.4 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM
Skeletal muscle	0.0 nTPM
Esophagus	0.0 nTPM

Fibroblasts	1.5 nTPM
Placenta	10.1 nTPM
Prostate	1.9 nTPM
Breast	1.8 nTPM
Esophagus	1.1 nTPM
Skin	0.7 nTPM
Ovary	0.6 nTPM
Heart muscle	0.2 nTPM
Adipose tissue	0.1 nTPM
Lung	0.0 nTPM
Skeletal muscle	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	31.4 nTPM
Small intestine	48.1 nTPM
Rectum	35.3 nTPM
Colon	10.7 nTPM

Macrophages	14.3 nTPM
Kidney	37.1 nTPM
Testis	33.1 nTPM
Breast	25.2 nTPM
Ovary	21.4 nTPM
Stomach	17.6 nTPM
Bone marrow	16.3 nTPM
Spleen	15.6 nTPM
Prostate	15.0 nTPM
Skeletal muscle	13.6 nTPM
Adipose tissue	7.3 nTPM
Endometrium	6.8 nTPM
Esophagus	4.8 nTPM
Lung	0.8 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM

Paneth cells	3.6 nTPM
Small intestine	3.7 nTPM
Rectum	3.7 nTPM
Colon	3.3 nTPM

Plasma cells	1.3 nTPM
Adipose tissue	3.3 nTPM
Bone marrow	1.7 nTPM
Stomach	1.0 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	1.0 nTPM
Lung	1.5 nTPM
Bronchus	0.5 nTPM

Smooth muscle cells	0.3 nTPM
Skin	1.0 nTPM
Adipose tissue	0.7 nTPM
Endometrium	0.6 nTPM
Prostate	0.4 nTPM
Pancreas	0.3 nTPM
Heart muscle	0.2 nTPM
Ovary	0.2 nTPM
Breast	0.2 nTPM
Bronchus	0.0 nTPM
Skeletal muscle	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	8.9 nTPM
Kidney	30.7 nTPM
PBMC	20.6 nTPM
Liver	20.4 nTPM
Ovary	20.1 nTPM
Skeletal muscle	16.7 nTPM
Bone marrow	14.0 nTPM
Prostate	9.5 nTPM
Adipose tissue	5.5 nTPM
Colon	3.3 nTPM
Spleen	3.2 nTPM
Endometrium	2.8 nTPM
Breast	2.1 nTPM
Stomach	1.0 nTPM
Skin	0.9 nTPM
Lung	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM

Basal prostatic cells	14.1 nTPM
Prostate	14.1 nTPM

Basal squamous epithelial cells	23.3 nTPM
Esophagus	23.3 nTPM

Collecting duct cells	20.2 nTPM
Kidney	20.2 nTPM

Cytotrophoblasts	18.7 nTPM
Placenta	18.7 nTPM

Undifferentiated cells	112.1 nTPM
Small intestine	175.9 nTPM
Colon	91.5 nTPM
Rectum	68.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC