Single cell type - ALKBH5 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ALKBH5
Gene descriptionⁱ Full gene name according to HGNC.	AlkB homolog 5, RNA demethylase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Basic cellular processes (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ALKBH5 is part of cluster 39 Non-specific - Basic cellular processes with confidenceⁱ 1
586 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ABHD14A	Abhydrolase domain containing 14A	0.4776	67
COX7A2L	Cytochrome c oxidase subunit 7A2 like	0.4760	39
TSR3	TSR3 ribosome maturation factor	0.4694	67
FAM50B	Family with sequence similarity 50 member B	0.4617	20
CDC26	Cell division cycle 26	0.4552	39
PNPLA4	Patatin like phospholipase domain containing 4	0.4476	67
GTPBP8	GTP binding protein 8 (putative)	0.4450	66
TIPIN	TIMELESS interacting protein	0.4437	67
OARD1	O-acyl-ADP-ribose deacylase 1	0.4375	20
PHYH	Phytanoyl-CoA 2-hydroxylase	0.4374	67
ACAT1	Acetyl-CoA acetyltransferase 1	0.4225	67
HOXC10	Homeobox C10	0.4220	67
MRPS23	Mitochondrial ribosomal protein S23	0.4213	39
RBAK-RBAKDN	RBAK-RBAKDN readthrough	0.4196	20
SAP18	Sin3A associated protein 18	0.4153	7

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	37.2 nTPM
Breast	43.8 nTPM
Adipose tissue	30.5 nTPM

Alveolar cells type 1	35.3 nTPM
Lung	35.3 nTPM

Alveolar cells type 2	33.3 nTPM
Lung	33.3 nTPM

Astrocytes	17.9 nTPM
Brain	17.9 nTPM

B-cells	41.1 nTPM
Colon	89.5 nTPM
PBMC	65.7 nTPM
Breast	62.8 nTPM
Liver	56.2 nTPM
Adipose tissue	43.1 nTPM
Lymph node	30.3 nTPM
Kidney	28.5 nTPM
Spleen	14.6 nTPM
Stomach	13.6 nTPM
Bone marrow	6.6 nTPM

Dendritic cells	59.0 nTPM
Breast	92.2 nTPM
Adipose tissue	56.9 nTPM
PBMC	27.9 nTPM

Endothelial cells	36.6 nTPM
Skin	51.4 nTPM
Liver	48.6 nTPM
Testis	46.5 nTPM
Ovary	45.2 nTPM
Prostate	45.0 nTPM
Breast	41.6 nTPM
Eye	40.5 nTPM
Endometrium	38.3 nTPM
Heart muscle	37.0 nTPM
Skeletal muscle	33.5 nTPM
Placenta	31.8 nTPM
Esophagus	30.4 nTPM
Lung	18.7 nTPM
Pancreas	3.9 nTPM

Fibroblasts	42.4 nTPM
Skin	80.4 nTPM
Prostate	79.6 nTPM
Heart muscle	51.3 nTPM
Skeletal muscle	41.9 nTPM
Adipose tissue	39.1 nTPM
Breast	38.7 nTPM
Placenta	33.7 nTPM
Ovary	31.6 nTPM
Lung	29.0 nTPM
Stomach	20.8 nTPM
Esophagus	20.0 nTPM

Intestinal goblet cells	45.0 nTPM
Rectum	48.5 nTPM
Colon	47.2 nTPM
Small intestine	39.2 nTPM

Macrophages	36.4 nTPM
Breast	67.0 nTPM
Endometrium	52.7 nTPM
Prostate	51.6 nTPM
Ovary	48.8 nTPM
Testis	42.4 nTPM
Adipose tissue	42.3 nTPM
PBMC	40.9 nTPM
Skeletal muscle	39.6 nTPM
Spleen	27.9 nTPM
Kidney	27.2 nTPM
Stomach	26.4 nTPM
Lung	23.2 nTPM
Pancreas	20.4 nTPM
Esophagus	20.4 nTPM
Bone marrow	15.0 nTPM

Paneth cells	38.7 nTPM
Small intestine	56.3 nTPM
Rectum	46.7 nTPM
Colon	13.2 nTPM

Plasma cells	21.1 nTPM
Adipose tissue	35.5 nTPM
Spleen	26.1 nTPM
Stomach	20.2 nTPM
Esophagus	12.3 nTPM
Bone marrow	11.5 nTPM

Respiratory ciliated cells	46.4 nTPM
Bronchus	62.1 nTPM
Lung	30.6 nTPM

Smooth muscle cells	47.3 nTPM
Skin	114.8 nTPM
Prostate	85.0 nTPM
Breast	55.9 nTPM
Skeletal muscle	50.3 nTPM
Pancreas	44.0 nTPM
Adipose tissue	40.1 nTPM
Endometrium	37.8 nTPM
Ovary	30.7 nTPM
Esophagus	27.9 nTPM
Heart muscle	20.3 nTPM
Bronchus	13.2 nTPM

T-cells	48.3 nTPM
Breast	115.5 nTPM
Colon	87.4 nTPM
Skin	84.8 nTPM
Adipose tissue	64.9 nTPM
PBMC	64.3 nTPM
Liver	59.0 nTPM
Kidney	56.9 nTPM
Skeletal muscle	56.7 nTPM
Ovary	51.2 nTPM
Endometrium	48.5 nTPM
Prostate	28.4 nTPM
Lymph node	27.4 nTPM
Spleen	21.0 nTPM
Stomach	14.9 nTPM
Lung	13.6 nTPM
Esophagus	13.1 nTPM
Bone marrow	13.0 nTPM

Basal keratinocytes	64.6 nTPM
Skin	64.6 nTPM

Basal respiratory cells	26.8 nTPM
Bronchus	26.8 nTPM

Basal squamous epithelial cells	19.4 nTPM
Esophagus	19.4 nTPM

Cholangiocytes	28.6 nTPM
Liver	28.6 nTPM

Undifferentiated cells	39.1 nTPM
Rectum	48.1 nTPM
Colon	35.6 nTPM
Small intestine	33.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC