Single cell type - MFNG - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MFNG
Gene descriptionⁱ Full gene name according to HGNC.	MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	NK-cells & T-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (NK-cells, dendritic cells, Hepatic stellate cells, Adipocytes, T-cells, Kupffer cells, monocytes, Endothelial cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Daudi, HEL, HL-60, HMC-1, HUVEC TERT2, TIME, U-698, U-937)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (NK-cells, dendritic cells, Hepatic stellate cells, Adipocytes, T-cells, Kupffer cells, monocytes, Endothelial cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

MFNG is part of cluster 63 NK-cells & T-cells - Immune response with confidenceⁱ 1
362 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
GIMAP1	GTPase, IMAP family member 1	0.8286	63
GMFG	Glia maturation factor gamma	0.8138	63
GIMAP7	GTPase, IMAP family member 7	0.7592	63
ANXA2R	Annexin A2 receptor	0.7484	63
GIMAP4	GTPase, IMAP family member 4	0.7358	63
GIMAP6	GTPase, IMAP family member 6	0.7320	63
GIMAP2	GTPase, IMAP family member 2	0.7158	63
BTN3A2	Butyrophilin subfamily 3 member A2	0.7121	63
CARD8	Caspase recruitment domain family member 8	0.6944	14
GPSM3	G protein signaling modulator 3	0.6911	63
TRAF3IP3	TRAF3 interacting protein 3	0.6791	63
TRAC	T cell receptor alpha constant	0.6747	63
HLA-E	Major histocompatibility complex, class I, E	0.6617	14
POLR3GL	RNA polymerase III subunit GL	0.6505	63
SNX20	Sorting nexin 20	0.6454	63

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	3.8 nTPM
Lung	3.8 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	35.4 nTPM
PBMC	65.7 nTPM
Liver	64.8 nTPM
Kidney	52.4 nTPM
Colon	33.5 nTPM
Breast	29.4 nTPM
Lymph node	27.1 nTPM
Spleen	26.3 nTPM
Stomach	21.8 nTPM
Adipose tissue	17.6 nTPM
Bone marrow	15.5 nTPM

Basal keratinocytes	3.7 nTPM
Skin	3.7 nTPM

Dendritic cells	54.6 nTPM
Breast	93.3 nTPM
Adipose tissue	46.6 nTPM
PBMC	23.9 nTPM

Endothelial cells	37.1 nTPM
Placenta	83.0 nTPM
Liver	78.9 nTPM
Breast	53.9 nTPM
Endometrium	39.2 nTPM
Skeletal muscle	38.0 nTPM
Esophagus	32.3 nTPM
Skin	30.3 nTPM
Heart muscle	28.0 nTPM
Eye	27.5 nTPM
Testis	27.2 nTPM
Prostate	26.5 nTPM
Pancreas	23.1 nTPM
Ovary	18.9 nTPM
Lung	12.5 nTPM

Fibroblasts	1.1 nTPM
Heart muscle	3.1 nTPM
Placenta	3.0 nTPM
Breast	2.3 nTPM
Skin	2.1 nTPM
Esophagus	0.8 nTPM
Skeletal muscle	0.7 nTPM
Adipose tissue	0.1 nTPM
Lung	0.0 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	2.1 nTPM
Rectum	2.7 nTPM
Colon	2.2 nTPM
Small intestine	1.4 nTPM

Macrophages	33.5 nTPM
PBMC	81.8 nTPM
Breast	45.6 nTPM
Kidney	42.4 nTPM
Ovary	40.0 nTPM
Testis	36.6 nTPM
Prostate	35.5 nTPM
Skeletal muscle	34.8 nTPM
Adipose tissue	29.8 nTPM
Endometrium	27.3 nTPM
Stomach	26.4 nTPM
Bone marrow	25.0 nTPM
Lung	21.5 nTPM
Pancreas	20.4 nTPM
Spleen	17.6 nTPM
Esophagus	17.3 nTPM

Paneth cells	4.0 nTPM
Small intestine	11.3 nTPM
Rectum	0.6 nTPM
Colon	0.0 nTPM

Plasma cells	35.0 nTPM
Esophagus	43.0 nTPM
Bone marrow	39.5 nTPM
Spleen	38.7 nTPM
Adipose tissue	29.7 nTPM
Stomach	24.0 nTPM

Respiratory ciliated cells	0.1 nTPM
Bronchus	0.2 nTPM
Lung	0.0 nTPM

Smooth muscle cells	1.0 nTPM
Breast	3.9 nTPM
Heart muscle	3.3 nTPM
Skin	2.4 nTPM
Endometrium	0.7 nTPM
Skeletal muscle	0.5 nTPM
Ovary	0.2 nTPM
Pancreas	0.1 nTPM
Prostate	0.0 nTPM
Bronchus	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	51.0 nTPM
PBMC	96.0 nTPM
Liver	72.2 nTPM
Kidney	69.8 nTPM
Breast	61.0 nTPM
Ovary	60.1 nTPM
Colon	58.5 nTPM
Lymph node	56.5 nTPM
Lung	56.2 nTPM
Skin	51.6 nTPM
Bone marrow	46.3 nTPM
Spleen	45.6 nTPM
Skeletal muscle	41.1 nTPM
Adipose tissue	38.4 nTPM
Stomach	35.4 nTPM
Esophagus	32.8 nTPM
Prostate	23.6 nTPM
Endometrium	22.3 nTPM

Cholangiocytes	5.7 nTPM
Liver	5.7 nTPM

Cytotrophoblasts	0.3 nTPM
Placenta	0.3 nTPM

Exocrine glandular cells	0.9 nTPM
Pancreas	0.9 nTPM

Undifferentiated cells	0.8 nTPM
Colon	1.2 nTPM
Rectum	0.7 nTPM
Small intestine	0.6 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC