Single cell type - PHKB - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PHKB
Gene descriptionⁱ Full gene name according to HGNC.	Phosphorylase kinase regulatory subunit beta
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	10

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PHKB is part of cluster 25 Spermatids - Spermatogenesis with confidenceⁱ 1
458 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
FGD4	FYVE, RhoGEF and PH domain containing 4	0.4738	41
ZKSCAN3	Zinc finger with KRAB and SCAN domains 3	0.4604	43
ANGEL1	Angel homolog 1	0.4531	25
DRD3	Dopamine receptor D3	0.4471	25
TEX2	Testis expressed 2	0.4224	21
CD200R1L	CD200 receptor 1 like	0.4218	25
KIAA1217	KIAA1217	0.4194	8
DHX57	DExH-box helicase 57	0.4190	25
UHRF1BP1	UHRF1 binding protein 1	0.4182	8
RMND5B	Required for meiotic nuclear division 5 homolog B	0.4166	25
UBE3B	Ubiquitin protein ligase E3B	0.4146	51
NBR1	NBR1 autophagy cargo receptor	0.4094	25
TSC1	TSC complex subunit 1	0.4085	25
QRFPR	Pyroglutamylated RFamide peptide receptor	0.4076	8
NETO2	Neuropilin and tolloid like 2	0.4071	8

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	49.3 nTPM
Adipose tissue	57.3 nTPM
Breast	41.2 nTPM

Alveolar cells type 1	19.2 nTPM
Lung	19.2 nTPM

Alveolar cells type 2	38.0 nTPM
Lung	38.0 nTPM

Astrocytes	66.9 nTPM
Brain	66.9 nTPM

B-cells	46.8 nTPM
PBMC	77.1 nTPM
Colon	54.3 nTPM
Adipose tissue	51.6 nTPM
Bone marrow	49.1 nTPM
Spleen	46.8 nTPM
Stomach	46.7 nTPM
Breast	44.1 nTPM
Lymph node	39.0 nTPM
Liver	35.1 nTPM
Kidney	23.8 nTPM

Dendritic cells	29.3 nTPM
PBMC	43.8 nTPM
Adipose tissue	25.8 nTPM
Breast	18.2 nTPM

Endothelial cells	42.9 nTPM
Heart muscle	69.2 nTPM
Skeletal muscle	62.8 nTPM
Lung	62.4 nTPM
Endometrium	53.1 nTPM
Ovary	47.6 nTPM
Testis	47.5 nTPM
Eye	46.1 nTPM
Esophagus	41.3 nTPM
Breast	37.3 nTPM
Placenta	36.4 nTPM
Prostate	25.6 nTPM
Liver	24.7 nTPM
Pancreas	23.1 nTPM
Skin	22.9 nTPM

Fibroblasts	28.8 nTPM
Skeletal muscle	44.9 nTPM
Heart muscle	38.5 nTPM
Esophagus	34.5 nTPM
Stomach	34.1 nTPM
Adipose tissue	34.0 nTPM
Ovary	28.3 nTPM
Lung	27.9 nTPM
Breast	26.2 nTPM
Placenta	23.5 nTPM
Skin	15.4 nTPM
Prostate	9.5 nTPM

Intestinal goblet cells	37.8 nTPM
Small intestine	45.0 nTPM
Rectum	38.9 nTPM
Colon	29.3 nTPM

Macrophages	45.1 nTPM
Ovary	78.1 nTPM
Spleen	56.0 nTPM
PBMC	54.6 nTPM
Esophagus	53.5 nTPM
Adipose tissue	52.9 nTPM
Bone marrow	51.2 nTPM
Skeletal muscle	48.1 nTPM
Testis	47.0 nTPM
Stomach	41.0 nTPM
Kidney	36.3 nTPM
Breast	34.0 nTPM
Pancreas	33.2 nTPM
Endometrium	33.2 nTPM
Lung	30.6 nTPM
Prostate	26.3 nTPM

Paneth cells	26.4 nTPM
Rectum	41.3 nTPM
Colon	23.1 nTPM
Small intestine	15.0 nTPM

Plasma cells	28.1 nTPM
Spleen	42.3 nTPM
Bone marrow	31.3 nTPM
Adipose tissue	29.7 nTPM
Stomach	24.7 nTPM
Esophagus	12.3 nTPM

Respiratory ciliated cells	26.7 nTPM
Bronchus	31.5 nTPM
Lung	21.9 nTPM

Smooth muscle cells	30.6 nTPM
Adipose tissue	51.4 nTPM
Skeletal muscle	49.3 nTPM
Endometrium	38.6 nTPM
Breast	37.5 nTPM
Ovary	35.3 nTPM
Heart muscle	35.1 nTPM
Esophagus	31.0 nTPM
Skin	17.5 nTPM
Prostate	15.3 nTPM
Pancreas	13.5 nTPM
Bronchus	12.2 nTPM

T-cells	24.7 nTPM
Skeletal muscle	42.3 nTPM
PBMC	40.5 nTPM
Endometrium	31.8 nTPM
Liver	30.8 nTPM
Adipose tissue	30.4 nTPM
Breast	28.2 nTPM
Lung	27.4 nTPM
Bone marrow	24.1 nTPM
Ovary	22.3 nTPM
Kidney	22.0 nTPM
Spleen	21.0 nTPM
Colon	20.9 nTPM
Stomach	20.0 nTPM
Lymph node	16.8 nTPM
Skin	14.3 nTPM
Prostate	14.2 nTPM
Esophagus	13.1 nTPM

Basal keratinocytes	8.9 nTPM
Skin	8.9 nTPM

Basal squamous epithelial cells	18.8 nTPM
Esophagus	18.8 nTPM

Cholangiocytes	32.9 nTPM
Liver	32.9 nTPM

Cone photoreceptor cells	100.1 nTPM
Eye	100.1 nTPM

Undifferentiated cells	38.6 nTPM
Rectum	43.7 nTPM
Small intestine	39.7 nTPM
Colon	32.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC