Single cell type - NT5DC3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NT5DC3
Gene descriptionⁱ Full gene name according to HGNC.	5'-nucleotidase domain containing 3
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Endometrium - Transcription (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Enteroendocrine cells, Excitatory neurons, Oligodendrocyte precursor cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (hTERT-HME1)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Enteroendocrine cells, Excitatory neurons, Oligodendrocyte precursor cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NT5DC3 is part of cluster 44 Endometrium - Transcription with confidenceⁱ 1
491 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
KCNG3	Potassium voltage-gated channel modifier subfamily G member 3	0.5778	44
HOXD13	Homeobox D13	0.5455	53
OPRK1	Opioid receptor kappa 1	0.5286	44
SYT13	Synaptotagmin 13	0.5264	17
VWA5B2	Von Willebrand factor A domain containing 5B2	0.5208	17
ZNF587	Zinc finger protein 587	0.5176	52
HOXB9	Homeobox B9	0.5162	17
EGR4	Early growth response 4	0.5153	17
TXNDC16	Thioredoxin domain containing 16	0.5126	44
MAP2K6	Mitogen-activated protein kinase kinase 6	0.5077	44
NEDD4L	NEDD4 like E3 ubiquitin protein ligase	0.5069	21
EPHB3	EPH receptor B3	0.5014	53
LEFTY1	Left-right determination factor 1	0.4968	53
USP43	Ubiquitin specific peptidase 43	0.4955	44
SLC38A11	Solute carrier family 38 member 11	0.4867	17

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.4 nTPM
Breast	1.4 nTPM
Adipose tissue	1.3 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	2.3 nTPM
Lung	2.3 nTPM

Astrocytes	3.9 nTPM
Brain	3.9 nTPM

B-cells	2.7 nTPM
Lymph node	4.7 nTPM
Breast	4.0 nTPM
Liver	3.7 nTPM
Spleen	3.5 nTPM
PBMC	2.8 nTPM
Bone marrow	2.5 nTPM
Adipose tissue	2.0 nTPM
Stomach	1.8 nTPM
Colon	1.6 nTPM
Kidney	0.0 nTPM

Dendritic cells	2.7 nTPM
PBMC	4.0 nTPM
Breast	2.2 nTPM
Adipose tissue	2.1 nTPM

Endothelial cells	1.7 nTPM
Prostate	3.5 nTPM
Testis	3.3 nTPM
Endometrium	3.2 nTPM
Skin	3.0 nTPM
Liver	2.7 nTPM
Esophagus	2.1 nTPM
Breast	1.7 nTPM
Heart muscle	1.3 nTPM
Skeletal muscle	1.3 nTPM
Eye	0.8 nTPM
Ovary	0.8 nTPM
Placenta	0.6 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM

Fibroblasts	3.8 nTPM
Placenta	6.5 nTPM
Adipose tissue	6.1 nTPM
Prostate	4.5 nTPM
Skeletal muscle	4.2 nTPM
Skin	3.9 nTPM
Lung	3.4 nTPM
Breast	3.3 nTPM
Stomach	3.0 nTPM
Ovary	2.8 nTPM
Esophagus	2.2 nTPM
Heart muscle	2.1 nTPM

Intestinal goblet cells	11.4 nTPM
Rectum	16.4 nTPM
Colon	12.9 nTPM
Small intestine	5.0 nTPM

Macrophages	2.8 nTPM
Adipose tissue	7.7 nTPM
Endometrium	7.2 nTPM
Skeletal muscle	4.4 nTPM
Testis	4.0 nTPM
Bone marrow	3.7 nTPM
Ovary	3.3 nTPM
Lung	2.9 nTPM
Breast	2.7 nTPM
Kidney	2.3 nTPM
Esophagus	1.6 nTPM
Prostate	1.2 nTPM
Spleen	1.0 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	2.2 nTPM
Rectum	6.7 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	2.7 nTPM
Stomach	5.9 nTPM
Spleen	4.0 nTPM
Adipose tissue	1.9 nTPM
Bone marrow	1.7 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	6.9 nTPM
Lung	10.2 nTPM
Bronchus	3.5 nTPM

Smooth muscle cells	5.7 nTPM
Skin	14.0 nTPM
Prostate	9.7 nTPM
Heart muscle	8.9 nTPM
Breast	8.3 nTPM
Endometrium	5.2 nTPM
Esophagus	4.6 nTPM
Skeletal muscle	3.4 nTPM
Ovary	3.4 nTPM
Pancreas	2.8 nTPM
Adipose tissue	2.1 nTPM
Bronchus	0.0 nTPM

T-cells	2.5 nTPM
Skeletal muscle	5.7 nTPM
Skin	5.5 nTPM
Prostate	4.7 nTPM
Endometrium	4.7 nTPM
Lung	4.5 nTPM
Breast	3.3 nTPM
Lymph node	3.2 nTPM
Adipose tissue	3.1 nTPM
Liver	2.6 nTPM
Spleen	1.8 nTPM
Stomach	1.5 nTPM
Colon	1.0 nTPM
PBMC	0.7 nTPM
Bone marrow	0.7 nTPM
Kidney	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Basal prostatic cells	21.9 nTPM
Prostate	21.9 nTPM

Basal squamous epithelial cells	21.6 nTPM
Esophagus	21.6 nTPM

Endometrial stromal cells	11.9 nTPM
Endometrium	11.9 nTPM

Undifferentiated cells	9.2 nTPM
Rectum	14.7 nTPM
Colon	9.2 nTPM
Small intestine	3.7 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC