Single cell type - AAK1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AAK1
Gene descriptionⁱ Full gene name according to HGNC.	AP2 associated kinase 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Photoreceptor cells - Phototransduction (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Cone photoreceptor cells, Rod photoreceptor cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Cone photoreceptor cells, Rod photoreceptor cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AAK1 is part of cluster 9 Photoreceptor cells - Phototransduction with confidenceⁱ 1
227 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
GLMN	Glomulin, FKBP associated protein	0.6034	46
GRIPAP1	GRIP1 associated protein 1	0.5904	9
ADCY1	Adenylate cyclase 1	0.5895	9
SRRM3	Serine/arginine repetitive matrix 3	0.5806	9
FAM126B	Family with sequence similarity 126 member B	0.5742	52
THEM4	Thioesterase superfamily member 4	0.5573	63
GON4L	Gon-4 like	0.5555	46
RPAP2	RNA polymerase II associated protein 2	0.5555	52
EYS	Eyes shut homolog	0.5541	9
BCO2	Beta-carotene oxygenase 2	0.5493	9
EVL	Enah/Vasp-like	0.5467	63
ABCG4	ATP binding cassette subfamily G member 4	0.5441	9
SFI1	SFI1 centrin binding protein	0.5430	52
DIP2A	Disco interacting protein 2 homolog A	0.5401	63
UBAP1L	Ubiquitin associated protein 1 like	0.5314	9

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	54.6 nTPM
Adipose tissue	64.0 nTPM
Breast	45.1 nTPM

Alveolar cells type 1	35.3 nTPM
Lung	35.3 nTPM

Alveolar cells type 2	34.2 nTPM
Lung	34.2 nTPM

Astrocytes	77.7 nTPM
Brain	77.7 nTPM

B-cells	13.4 nTPM
Liver	45.9 nTPM
Breast	24.1 nTPM
Adipose tissue	17.6 nTPM
PBMC	17.1 nTPM
Lymph node	8.3 nTPM
Bone marrow	6.6 nTPM
Colon	6.4 nTPM
Spleen	5.5 nTPM
Stomach	2.7 nTPM
Kidney	0.0 nTPM

Dendritic cells	149.1 nTPM
Adipose tissue	267.6 nTPM
Breast	159.6 nTPM
PBMC	20.0 nTPM

Endothelial cells	56.0 nTPM
Eye	112.5 nTPM
Testis	101.6 nTPM
Heart muscle	97.9 nTPM
Liver	65.1 nTPM
Endometrium	58.4 nTPM
Breast	55.9 nTPM
Skeletal muscle	54.2 nTPM
Lung	46.8 nTPM
Esophagus	40.8 nTPM
Placenta	38.0 nTPM
Ovary	33.7 nTPM
Pancreas	30.9 nTPM
Prostate	24.1 nTPM
Skin	24.1 nTPM

Fibroblasts	29.5 nTPM
Breast	50.3 nTPM
Adipose tissue	42.6 nTPM
Heart muscle	36.1 nTPM
Lung	35.7 nTPM
Skeletal muscle	30.4 nTPM
Prostate	27.8 nTPM
Placenta	27.5 nTPM
Ovary	20.7 nTPM
Skin	19.7 nTPM
Esophagus	17.7 nTPM
Stomach	16.3 nTPM

Intestinal goblet cells	31.6 nTPM
Colon	34.3 nTPM
Rectum	31.2 nTPM
Small intestine	29.4 nTPM

Macrophages	67.1 nTPM
Ovary	141.2 nTPM
Adipose tissue	96.5 nTPM
Breast	84.8 nTPM
Testis	84.2 nTPM
Lung	74.2 nTPM
Endometrium	71.4 nTPM
Bone marrow	71.2 nTPM
Spleen	68.5 nTPM
Skeletal muscle	67.2 nTPM
Stomach	61.5 nTPM
Kidney	53.7 nTPM
Esophagus	53.5 nTPM
Pancreas	46.0 nTPM
Prostate	32.0 nTPM
PBMC	0.0 nTPM

Paneth cells	76.3 nTPM
Colon	181.2 nTPM
Rectum	29.1 nTPM
Small intestine	18.8 nTPM

Plasma cells	57.4 nTPM
Adipose tissue	187.0 nTPM
Bone marrow	44.5 nTPM
Spleen	37.1 nTPM
Stomach	18.4 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	10.7 nTPM
Lung	11.7 nTPM
Bronchus	9.7 nTPM

Smooth muscle cells	45.5 nTPM
Adipose tissue	91.9 nTPM
Endometrium	88.5 nTPM
Pancreas	53.1 nTPM
Breast	49.4 nTPM
Heart muscle	48.4 nTPM
Skeletal muscle	44.2 nTPM
Ovary	41.4 nTPM
Prostate	32.8 nTPM
Esophagus	31.0 nTPM
Skin	18.1 nTPM
Bronchus	1.8 nTPM

T-cells	158.1 nTPM
PBMC	368.6 nTPM
Liver	283.6 nTPM
Adipose tissue	240.4 nTPM
Breast	207.3 nTPM
Skeletal muscle	181.1 nTPM
Endometrium	176.9 nTPM
Lymph node	163.4 nTPM
Bone marrow	150.3 nTPM
Colon	146.8 nTPM
Ovary	144.6 nTPM
Kidney	141.0 nTPM
Spleen	127.7 nTPM
Stomach	105.1 nTPM
Lung	85.0 nTPM
Esophagus	72.0 nTPM
Skin	65.4 nTPM
Prostate	28.4 nTPM

Basal keratinocytes	15.9 nTPM
Skin	15.9 nTPM

Basal prostatic cells	20.8 nTPM
Prostate	20.8 nTPM

Basal respiratory cells	15.9 nTPM
Bronchus	15.9 nTPM

Basal squamous epithelial cells	32.5 nTPM
Esophagus	32.5 nTPM

Undifferentiated cells	21.7 nTPM
Rectum	27.0 nTPM
Colon	24.3 nTPM
Small intestine	13.7 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC