Single cell type - PFKFB2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PFKFB2
Gene descriptionⁱ Full gene name according to HGNC.	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Plasmacytoid DCs - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Rod photoreceptor cells, Astrocytes, Cardiomyocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (plasmacytoid DC, eosinophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Rod photoreceptor cells, Astrocytes, Cardiomyocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PFKFB2 is part of cluster 65 Plasmacytoid DCs - Unknown function with confidenceⁱ 1
144 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
USP2	Ubiquitin specific peptidase 2	0.5251	31
MRPS25	Mitochondrial ribosomal protein S25	0.5234	31
ANKRD53	Ankyrin repeat domain 53	0.5232	25
ANO2	Anoctamin 2	0.5218	46
OSBP2	Oxysterol binding protein 2	0.5131	46
WFDC10B	WAP four-disulfide core domain 10B	0.5035	46
GUCA1A	Guanylate cyclase activator 1A	0.4893	46
PPARA	Peroxisome proliferator activated receptor alpha	0.4883	46
ESRRB	Estrogen related receptor beta	0.4644	46
MYO9A	Myosin IXA	0.4597	46
DEFB119	Defensin beta 119	0.4594	46
GHITM	Growth hormone inducible transmembrane protein	0.4482	39
NDUFA9	NADH:ubiquinone oxidoreductase subunit A9	0.4452	25
KRT71	Keratin 71	0.4436	65
CIB2	Calcium and integrin binding family member 2	0.4420	25

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.0 nTPM
Breast	1.1 nTPM
Adipose tissue	0.9 nTPM

Alveolar cells type 1	6.4 nTPM
Lung	6.4 nTPM

Alveolar cells type 2	54.8 nTPM
Lung	54.8 nTPM

B-cells	5.0 nTPM
PBMC	8.5 nTPM
Breast	6.6 nTPM
Adipose tissue	5.0 nTPM
Colon	4.8 nTPM
Kidney	4.8 nTPM
Lymph node	4.6 nTPM
Liver	4.2 nTPM
Bone marrow	4.1 nTPM
Spleen	3.8 nTPM
Stomach	3.2 nTPM

Basal keratinocytes	15.2 nTPM
Skin	15.2 nTPM

Dendritic cells	24.6 nTPM
PBMC	67.8 nTPM
Adipose tissue	4.5 nTPM
Breast	1.5 nTPM

Endothelial cells	4.2 nTPM
Heart muscle	27.4 nTPM
Eye	7.3 nTPM
Lung	6.2 nTPM
Skin	3.1 nTPM
Placenta	2.9 nTPM
Endometrium	2.7 nTPM
Ovary	2.4 nTPM
Prostate	2.1 nTPM
Breast	2.0 nTPM
Skeletal muscle	1.2 nTPM
Testis	1.1 nTPM
Esophagus	0.6 nTPM
Liver	0.0 nTPM
Pancreas	0.0 nTPM

Fibroblasts	4.6 nTPM
Heart muscle	20.9 nTPM
Ovary	6.9 nTPM
Lung	5.6 nTPM
Skin	3.8 nTPM
Prostate	2.7 nTPM
Placenta	2.3 nTPM
Breast	2.2 nTPM
Esophagus	2.1 nTPM
Skeletal muscle	1.7 nTPM
Stomach	1.5 nTPM
Adipose tissue	1.4 nTPM

Intestinal goblet cells	20.1 nTPM
Rectum	28.3 nTPM
Small intestine	19.1 nTPM
Colon	12.9 nTPM

Macrophages	8.5 nTPM
Esophagus	20.4 nTPM
Bone marrow	15.0 nTPM
Kidney	11.4 nTPM
Lung	10.9 nTPM
Ovary	10.1 nTPM
Spleen	9.3 nTPM
Endometrium	9.2 nTPM
Breast	8.7 nTPM
Testis	8.1 nTPM
Skeletal muscle	6.7 nTPM
Stomach	5.9 nTPM
Adipose tissue	5.2 nTPM
Prostate	4.6 nTPM
Pancreas	2.5 nTPM
PBMC	0.0 nTPM

Paneth cells	26.2 nTPM
Colon	32.9 nTPM
Rectum	30.6 nTPM
Small intestine	15.0 nTPM

Plasma cells	3.8 nTPM
Esophagus	12.3 nTPM
Stomach	3.4 nTPM
Adipose tissue	2.7 nTPM
Spleen	0.7 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	15.3 nTPM
Lung	16.0 nTPM
Bronchus	14.7 nTPM

Smooth muscle cells	3.1 nTPM
Skin	7.0 nTPM
Endometrium	4.4 nTPM
Prostate	4.3 nTPM
Heart muscle	3.5 nTPM
Esophagus	3.1 nTPM
Breast	2.8 nTPM
Ovary	2.2 nTPM
Skeletal muscle	2.1 nTPM
Bronchus	1.8 nTPM
Adipose tissue	1.4 nTPM
Pancreas	1.2 nTPM

T-cells	1.6 nTPM
Prostate	4.7 nTPM
Lung	4.5 nTPM
Endometrium	3.0 nTPM
Lymph node	2.5 nTPM
Colon	2.4 nTPM
PBMC	1.5 nTPM
Kidney	1.4 nTPM
Breast	1.4 nTPM
Skeletal muscle	1.3 nTPM
Spleen	1.0 nTPM
Bone marrow	0.9 nTPM
Liver	0.6 nTPM
Adipose tissue	0.6 nTPM
Stomach	0.5 nTPM
Skin	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Astrocytes	105.1 nTPM
Brain	105.1 nTPM

Collecting duct cells	16.5 nTPM
Kidney	16.5 nTPM

Distal enterocytes	53.8 nTPM
Rectum	61.1 nTPM
Colon	46.5 nTPM

Distal tubular cells	25.4 nTPM
Kidney	25.4 nTPM

Undifferentiated cells	36.9 nTPM
Rectum	42.5 nTPM
Colon	39.8 nTPM
Small intestine	28.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC