Single cell type - EEF1E1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	EEF1E1
Gene descriptionⁱ Full gene name according to HGNC.	Eukaryotic translation elongation factor 1 epsilon 1
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Early spermatids, Late spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Early spermatids, Late spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

EEF1E1 is part of cluster 34 Spermatids - Unknown function with confidenceⁱ 1
366 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PSPH	Phosphoserine phosphatase	0.5750	34
PSMA4	Proteasome 20S subunit alpha 4	0.5522	26
PSME3	Proteasome activator subunit 3	0.5332	34
PES1	Pescadillo ribosomal biogenesis factor 1	0.5219	39
MPLKIP	M-phase specific PLK1 interacting protein	0.5197	39
BAG5	BAG cochaperone 5	0.5188	34
TSFM	Ts translation elongation factor, mitochondrial	0.5185	39
LYPLA1	Lysophospholipase 1	0.5039	34
MRPL19	Mitochondrial ribosomal protein L19	0.5026	39
MRPL17	Mitochondrial ribosomal protein L17	0.4971	39
MTIF3	Mitochondrial translational initiation factor 3	0.4945	39
TFAM	Transcription factor A, mitochondrial	0.4940	34
ACP1	Acid phosphatase 1	0.4838	34
LSM3	LSM3 homolog, U6 small nuclear RNA and mRNA degradation associated	0.4835	67
MRPL42	Mitochondrial ribosomal protein L42	0.4826	39

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	31.9 nTPM
Breast	42.9 nTPM
Adipose tissue	20.9 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	30.0 nTPM
Lung	30.0 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	39.7 nTPM
Liver	87.2 nTPM
Spleen	44.7 nTPM
PBMC	41.4 nTPM
Lymph node	38.3 nTPM
Stomach	35.4 nTPM
Bone marrow	35.1 nTPM
Breast	34.7 nTPM
Colon	31.9 nTPM
Kidney	28.5 nTPM
Adipose tissue	20.1 nTPM

Dendritic cells	23.5 nTPM
Breast	35.3 nTPM
Adipose tissue	19.2 nTPM
PBMC	16.0 nTPM

Endothelial cells	34.1 nTPM
Pancreas	69.5 nTPM
Skin	52.9 nTPM
Placenta	52.3 nTPM
Prostate	48.6 nTPM
Testis	35.3 nTPM
Breast	31.5 nTPM
Endometrium	31.4 nTPM
Ovary	29.7 nTPM
Skeletal muscle	28.0 nTPM
Esophagus	27.3 nTPM
Eye	24.2 nTPM
Heart muscle	19.8 nTPM
Liver	14.7 nTPM
Lung	12.5 nTPM

Fibroblasts	30.8 nTPM
Ovary	53.4 nTPM
Placenta	48.5 nTPM
Prostate	35.7 nTPM
Stomach	34.1 nTPM
Skin	32.2 nTPM
Breast	27.1 nTPM
Adipose tissue	25.3 nTPM
Skeletal muscle	22.7 nTPM
Heart muscle	21.5 nTPM
Lung	20.1 nTPM
Esophagus	17.7 nTPM

Intestinal goblet cells	36.6 nTPM
Colon	52.1 nTPM
Rectum	33.6 nTPM
Small intestine	24.2 nTPM

Macrophages	31.3 nTPM
Kidney	62.8 nTPM
Pancreas	51.0 nTPM
Testis	46.4 nTPM
Endometrium	37.2 nTPM
Prostate	34.4 nTPM
Breast	31.7 nTPM
PBMC	27.3 nTPM
Esophagus	26.8 nTPM
Bone marrow	26.2 nTPM
Ovary	23.5 nTPM
Lung	23.3 nTPM
Spleen	22.8 nTPM
Adipose tissue	20.5 nTPM
Stomach	17.6 nTPM
Skeletal muscle	17.4 nTPM

Paneth cells	12.9 nTPM
Rectum	31.2 nTPM
Small intestine	7.5 nTPM
Colon	0.0 nTPM

Plasma cells	48.0 nTPM
Spleen	87.5 nTPM
Bone marrow	80.7 nTPM
Stomach	33.8 nTPM
Esophagus	30.7 nTPM
Adipose tissue	7.0 nTPM

Respiratory ciliated cells	45.9 nTPM
Bronchus	55.3 nTPM
Lung	36.5 nTPM

Smooth muscle cells	38.9 nTPM
Pancreas	86.2 nTPM
Ovary	51.6 nTPM
Prostate	41.8 nTPM
Skin	38.7 nTPM
Heart muscle	35.4 nTPM
Bronchus	34.7 nTPM
Breast	34.6 nTPM
Esophagus	29.4 nTPM
Endometrium	29.0 nTPM
Skeletal muscle	27.7 nTPM
Adipose tissue	18.1 nTPM

T-cells	34.1 nTPM
PBMC	52.0 nTPM
Kidney	48.6 nTPM
Skin	40.9 nTPM
Ovary	40.0 nTPM
Esophagus	39.3 nTPM
Lymph node	38.4 nTPM
Bone marrow	36.3 nTPM
Liver	34.2 nTPM
Colon	33.7 nTPM
Breast	33.1 nTPM
Endometrium	32.1 nTPM
Spleen	31.7 nTPM
Stomach	29.6 nTPM
Lung	28.8 nTPM
Skeletal muscle	21.1 nTPM
Adipose tissue	20.1 nTPM
Prostate	19.0 nTPM

Basal keratinocytes	65.9 nTPM
Skin	65.9 nTPM

Basal prostatic cells	61.4 nTPM
Prostate	61.4 nTPM

Basal respiratory cells	99.1 nTPM
Bronchus	99.1 nTPM

Basal squamous epithelial cells	60.3 nTPM
Esophagus	60.3 nTPM

Undifferentiated cells	42.0 nTPM
Colon	52.5 nTPM
Small intestine	41.2 nTPM
Rectum	32.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC