Single cell type - TGS1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TGS1
Gene descriptionⁱ Full gene name according to HGNC.	Trimethylguanosine synthase 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Transcription (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TGS1 is part of cluster 59 Non-specific - Transcription with confidenceⁱ 0.98
354 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
GTF2H3	General transcription factor IIH subunit 3	0.6506	59
TTC4	Tetratricopeptide repeat domain 4	0.6145	59
WDR3	WD repeat domain 3	0.5760	13
PALB2	Partner and localizer of BRCA2	0.5683	13
POLR1E	RNA polymerase I subunit E	0.5548	32
NOC3L	NOC3 like DNA replication regulator	0.5382	59
LCMT2	Leucine carboxyl methyltransferase 2	0.5378	13
UTP20	UTP20 small subunit processome component	0.5364	59
NAT10	N-acetyltransferase 10	0.5354	59
ASB3	Ankyrin repeat and SOCS box containing 3	0.5321	59
ZNF557	Zinc finger protein 557	0.5294	52
ZMYM1	Zinc finger MYM-type containing 1	0.5250	59
ICE1	Interactor of little elongation complex ELL subunit 1	0.5210	52
DPH7	Diphthamide biosynthesis 7	0.5189	13
NOL8	Nucleolar protein 8	0.5177	52

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	29.8 nTPM
Breast	32.8 nTPM
Adipose tissue	26.7 nTPM

Alveolar cells type 1	48.1 nTPM
Lung	48.1 nTPM

Alveolar cells type 2	35.2 nTPM
Lung	35.2 nTPM

Astrocytes	15.7 nTPM
Brain	15.7 nTPM

B-cells	41.6 nTPM
Stomach	60.9 nTPM
Spleen	55.0 nTPM
Lymph node	49.6 nTPM
Kidney	42.9 nTPM
PBMC	40.0 nTPM
Adipose tissue	38.6 nTPM
Colon	36.7 nTPM
Bone marrow	32.7 nTPM
Breast	32.1 nTPM
Liver	27.4 nTPM

Dendritic cells	30.4 nTPM
Adipose tissue	45.8 nTPM
Breast	41.2 nTPM
PBMC	4.0 nTPM

Endothelial cells	27.2 nTPM
Skeletal muscle	43.9 nTPM
Esophagus	39.1 nTPM
Liver	31.2 nTPM
Lung	31.2 nTPM
Testis	30.2 nTPM
Eye	28.3 nTPM
Breast	27.8 nTPM
Pancreas	27.0 nTPM
Endometrium	21.7 nTPM
Ovary	21.7 nTPM
Heart muscle	21.6 nTPM
Prostate	21.1 nTPM
Skin	18.1 nTPM
Placenta	17.6 nTPM

Fibroblasts	32.4 nTPM
Lung	48.0 nTPM
Adipose tissue	43.6 nTPM
Skeletal muscle	41.7 nTPM
Esophagus	40.5 nTPM
Stomach	38.6 nTPM
Breast	31.2 nTPM
Placenta	27.4 nTPM
Prostate	27.2 nTPM
Ovary	22.9 nTPM
Heart muscle	19.0 nTPM
Skin	16.0 nTPM

Intestinal goblet cells	23.3 nTPM
Colon	25.7 nTPM
Small intestine	23.2 nTPM
Rectum	21.0 nTPM

Macrophages	22.2 nTPM
Stomach	35.1 nTPM
Lung	29.2 nTPM
Breast	28.4 nTPM
Spleen	27.9 nTPM
Kidney	26.5 nTPM
Ovary	23.8 nTPM
Esophagus	23.6 nTPM
Testis	23.2 nTPM
Skeletal muscle	22.6 nTPM
Bone marrow	22.5 nTPM
Endometrium	21.2 nTPM
Adipose tissue	19.3 nTPM
Prostate	17.2 nTPM
Pancreas	12.8 nTPM
PBMC	0.0 nTPM

Paneth cells	12.1 nTPM
Rectum	21.6 nTPM
Small intestine	11.3 nTPM
Colon	3.3 nTPM

Plasma cells	32.7 nTPM
Spleen	50.4 nTPM
Bone marrow	41.2 nTPM
Stomach	26.5 nTPM
Esophagus	24.6 nTPM
Adipose tissue	20.6 nTPM

Respiratory ciliated cells	25.5 nTPM
Lung	36.5 nTPM
Bronchus	14.5 nTPM

Smooth muscle cells	25.7 nTPM
Heart muscle	41.7 nTPM
Skeletal muscle	34.0 nTPM
Esophagus	32.5 nTPM
Breast	28.5 nTPM
Endometrium	28.2 nTPM
Adipose tissue	24.9 nTPM
Ovary	24.7 nTPM
Skin	21.6 nTPM
Pancreas	21.0 nTPM
Prostate	18.1 nTPM
Bronchus	7.6 nTPM

T-cells	41.2 nTPM
Stomach	59.8 nTPM
PBMC	54.4 nTPM
Spleen	49.8 nTPM
Bone marrow	47.8 nTPM
Adipose tissue	45.5 nTPM
Kidney	43.8 nTPM
Lymph node	43.8 nTPM
Skeletal muscle	43.3 nTPM
Endometrium	40.6 nTPM
Colon	40.4 nTPM
Breast	40.2 nTPM
Lung	39.5 nTPM
Ovary	37.8 nTPM
Prostate	33.1 nTPM
Liver	30.4 nTPM
Esophagus	26.2 nTPM
Skin	23.5 nTPM

Basal keratinocytes	37.0 nTPM
Skin	37.0 nTPM

Basal prostatic cells	36.8 nTPM
Prostate	36.8 nTPM

Basal squamous epithelial cells	35.3 nTPM
Esophagus	35.3 nTPM

Cholangiocytes	20.0 nTPM
Liver	20.0 nTPM

Undifferentiated cells	22.5 nTPM
Colon	23.8 nTPM
Small intestine	23.6 nTPM
Rectum	20.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC