Single cell type - AFG3L2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AFG3L2
Gene descriptionⁱ Full gene name according to HGNC.	AFG3 like matrix AAA peptidase subunit 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Plasma proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Intestinal epithelial cells - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AFG3L2 is part of cluster 53 Intestinal epithelial cells - Unknown function with confidenceⁱ 1
611 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SUCLG2	Succinate-CoA ligase GDP-forming subunit beta	0.6135	53
CHCHD10	Coiled-coil-helix-coiled-coil-helix domain containing 10	0.6093	39
NDUFA10	NADH:ubiquinone oxidoreductase subunit A10	0.6073	39
TMEM141	Transmembrane protein 141	0.6037	53
UQCRC1	Ubiquinol-cytochrome c reductase core protein 1	0.5903	39
TCEA3	Transcription elongation factor A3	0.5874	53
MRPL12	Mitochondrial ribosomal protein L12	0.5845	53
CDX1	Caudal type homeobox 1	0.5727	53
NOX1	NADPH oxidase 1	0.5668	53
SLC28A2	Solute carrier family 28 member 2	0.5651	53
CYC1	Cytochrome c1	0.5631	39
SURF6	Surfeit 6	0.5623	53
SIAE	Sialic acid acetylesterase	0.5616	53
DECR2	2,4-dienoyl-CoA reductase 2	0.5531	67
NXPE4	Neurexophilin and PC-esterase domain family member 4	0.5504	53

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	24.3 nTPM
Adipose tissue	28.0 nTPM
Breast	20.7 nTPM

Alveolar cells type 1	41.7 nTPM
Lung	41.7 nTPM

Alveolar cells type 2	49.2 nTPM
Lung	49.2 nTPM

Astrocytes	23.2 nTPM
Brain	23.2 nTPM

B-cells	36.6 nTPM
Adipose tissue	51.2 nTPM
Colon	46.4 nTPM
Liver	46.3 nTPM
Spleen	39.4 nTPM
Bone marrow	36.8 nTPM
Stomach	36.8 nTPM
Lymph node	35.2 nTPM
PBMC	28.5 nTPM
Breast	26.8 nTPM
Kidney	19.0 nTPM

Dendritic cells	29.1 nTPM
Adipose tissue	49.6 nTPM
Breast	21.8 nTPM
PBMC	16.0 nTPM

Endothelial cells	27.7 nTPM
Heart muscle	36.8 nTPM
Testis	35.0 nTPM
Endometrium	34.8 nTPM
Breast	30.2 nTPM
Eye	29.9 nTPM
Esophagus	29.4 nTPM
Skeletal muscle	28.5 nTPM
Pancreas	27.0 nTPM
Ovary	26.6 nTPM
Lung	24.9 nTPM
Liver	24.7 nTPM
Prostate	22.1 nTPM
Placenta	21.6 nTPM
Skin	15.7 nTPM

Fibroblasts	31.2 nTPM
Lung	50.3 nTPM
Heart muscle	44.2 nTPM
Adipose tissue	39.1 nTPM
Ovary	32.3 nTPM
Skeletal muscle	30.0 nTPM
Stomach	28.2 nTPM
Esophagus	27.9 nTPM
Prostate	27.0 nTPM
Placenta	26.8 nTPM
Breast	21.8 nTPM
Skin	15.8 nTPM

Intestinal goblet cells	82.6 nTPM
Colon	97.2 nTPM
Rectum	79.3 nTPM
Small intestine	71.2 nTPM

Macrophages	34.2 nTPM
Pancreas	61.2 nTPM
Ovary	45.2 nTPM
Adipose tissue	43.3 nTPM
Lung	42.1 nTPM
Esophagus	37.7 nTPM
Testis	34.9 nTPM
Endometrium	34.2 nTPM
Stomach	32.2 nTPM
Spleen	32.1 nTPM
Bone marrow	31.2 nTPM
Skeletal muscle	30.8 nTPM
Prostate	26.3 nTPM
Kidney	24.2 nTPM
Breast	23.4 nTPM
PBMC	13.6 nTPM

Paneth cells	43.1 nTPM
Rectum	70.6 nTPM
Colon	36.2 nTPM
Small intestine	22.5 nTPM

Plasma cells	38.8 nTPM
Bone marrow	51.1 nTPM
Spleen	44.8 nTPM
Adipose tissue	35.5 nTPM
Stomach	32.0 nTPM
Esophagus	30.7 nTPM

Respiratory ciliated cells	22.1 nTPM
Lung	30.6 nTPM
Bronchus	13.5 nTPM

Smooth muscle cells	26.9 nTPM
Esophagus	41.9 nTPM
Endometrium	41.5 nTPM
Skeletal muscle	34.6 nTPM
Ovary	34.2 nTPM
Pancreas	32.2 nTPM
Adipose tissue	29.6 nTPM
Prostate	23.5 nTPM
Breast	20.5 nTPM
Heart muscle	18.7 nTPM
Skin	15.3 nTPM
Bronchus	3.7 nTPM

T-cells	28.4 nTPM
Adipose tissue	48.1 nTPM
Endometrium	38.8 nTPM
Stomach	37.3 nTPM
Bone marrow	35.0 nTPM
PBMC	34.9 nTPM
Colon	34.7 nTPM
Lymph node	33.5 nTPM
Skeletal muscle	30.4 nTPM
Liver	29.8 nTPM
Spleen	27.7 nTPM
Kidney	23.3 nTPM
Breast	22.3 nTPM
Lung	21.3 nTPM
Ovary	20.1 nTPM
Prostate	19.0 nTPM
Skin	14.3 nTPM
Esophagus	13.1 nTPM

Basal keratinocytes	22.2 nTPM
Skin	22.2 nTPM

Basal prostatic cells	45.3 nTPM
Prostate	45.3 nTPM

Basal respiratory cells	21.5 nTPM
Bronchus	21.5 nTPM

Cholangiocytes	50.1 nTPM
Liver	50.1 nTPM

Undifferentiated cells	94.9 nTPM
Colon	112.1 nTPM
Rectum	92.7 nTPM
Small intestine	79.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC