Single cell type - EBP - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	EBP
Gene descriptionⁱ Full gene name according to HGNC.	EBP cholestenol delta-isomerase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Hepatocytes - Hemostasis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Hepatocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Hepatocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

EBP is part of cluster 15 Hepatocytes - Hemostasis with confidenceⁱ 1
129 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
MSMO1	Methylsterol monooxygenase 1	0.7799	15
SAA2	Serum amyloid A2	0.7689	15
SAA1	Serum amyloid A1	0.7677	15
LRG1	Leucine rich alpha-2-glycoprotein 1	0.7658	15
SAA2-SAA4	SAA2-SAA4 readthrough	0.7482	15
SERPINA1	Serpin family A member 1	0.7436	15
LBP	Lipopolysaccharide binding protein	0.7418	15
ANGPTL8	Angiopoietin like 8	0.7313	15
ACAT2	Acetyl-CoA acetyltransferase 2	0.7275	26
CRP	C-reactive protein	0.7264	15
CFHR1	Complement factor H related 1	0.7231	15
APOC4	Apolipoprotein C4	0.7220	15
ITIH3	Inter-alpha-trypsin inhibitor heavy chain 3	0.7203	15
CFHR4	Complement factor H related 4	0.7202	15
FGG	Fibrinogen gamma chain	0.7194	15

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	15.2 nTPM
Adipose tissue	17.5 nTPM
Breast	12.8 nTPM

Alveolar cells type 1	32.1 nTPM
Lung	32.1 nTPM

Alveolar cells type 2	49.8 nTPM
Lung	49.8 nTPM

Astrocytes	2.9 nTPM
Brain	2.9 nTPM

B-cells	38.9 nTPM
Liver	125.6 nTPM
Colon	46.4 nTPM
Kidney	42.9 nTPM
Adipose tissue	38.1 nTPM
Breast	37.5 nTPM
PBMC	34.3 nTPM
Spleen	19.7 nTPM
Stomach	18.6 nTPM
Lymph node	14.0 nTPM
Bone marrow	12.3 nTPM

Dendritic cells	74.5 nTPM
Adipose tissue	106.1 nTPM
Breast	89.3 nTPM
PBMC	27.9 nTPM

Endothelial cells	26.1 nTPM
Pancreas	54.0 nTPM
Placenta	43.2 nTPM
Ovary	35.4 nTPM
Lung	34.3 nTPM
Liver	30.2 nTPM
Testis	26.4 nTPM
Prostate	21.8 nTPM
Skin	21.4 nTPM
Breast	20.7 nTPM
Heart muscle	20.2 nTPM
Eye	17.0 nTPM
Endometrium	16.8 nTPM
Skeletal muscle	13.3 nTPM
Esophagus	10.8 nTPM

Fibroblasts	21.4 nTPM
Placenta	45.9 nTPM
Heart muscle	35.3 nTPM
Ovary	33.4 nTPM
Skin	31.6 nTPM
Adipose tissue	20.7 nTPM
Breast	17.5 nTPM
Skeletal muscle	14.6 nTPM
Prostate	13.3 nTPM
Esophagus	10.9 nTPM
Lung	6.7 nTPM
Stomach	6.0 nTPM

Intestinal goblet cells	96.8 nTPM
Colon	137.9 nTPM
Small intestine	79.6 nTPM
Rectum	72.9 nTPM

Macrophages	31.9 nTPM
Pancreas	102.0 nTPM
Prostate	57.3 nTPM
Ovary	43.2 nTPM
Kidney	41.5 nTPM
Breast	31.8 nTPM
Adipose tissue	31.7 nTPM
Lung	29.9 nTPM
Testis	28.4 nTPM
Endometrium	26.0 nTPM
Skeletal muscle	18.4 nTPM
Esophagus	17.3 nTPM
Stomach	14.7 nTPM
PBMC	13.6 nTPM
Bone marrow	12.5 nTPM
Spleen	10.4 nTPM

Paneth cells	137.3 nTPM
Colon	168.0 nTPM
Small intestine	138.9 nTPM
Rectum	105.0 nTPM

Plasma cells	54.4 nTPM
Spleen	77.1 nTPM
Adipose tissue	60.0 nTPM
Bone marrow	59.4 nTPM
Stomach	38.7 nTPM
Esophagus	36.9 nTPM

Respiratory ciliated cells	34.8 nTPM
Bronchus	44.8 nTPM
Lung	24.8 nTPM

Smooth muscle cells	23.6 nTPM
Bronchus	58.1 nTPM
Pancreas	54.3 nTPM
Ovary	33.0 nTPM
Heart muscle	29.7 nTPM
Prostate	16.6 nTPM
Skin	15.7 nTPM
Breast	14.8 nTPM
Adipose tissue	14.3 nTPM
Endometrium	12.3 nTPM
Skeletal muscle	9.2 nTPM
Esophagus	1.5 nTPM

T-cells	57.1 nTPM
Prostate	99.4 nTPM
Kidney	92.4 nTPM
Ovary	82.3 nTPM
Colon	81.2 nTPM
Adipose tissue	78.3 nTPM
Skin	76.0 nTPM
Liver	73.3 nTPM
Breast	59.0 nTPM
Endometrium	53.7 nTPM
Skeletal muscle	47.0 nTPM
PBMC	39.6 nTPM
Bone marrow	38.2 nTPM
Stomach	36.9 nTPM
Spleen	32.0 nTPM
Lung	31.9 nTPM
Esophagus	26.2 nTPM
Lymph node	24.1 nTPM

Basal keratinocytes	37.1 nTPM
Skin	37.1 nTPM

Basal prostatic cells	58.2 nTPM
Prostate	58.2 nTPM

Basal respiratory cells	245.6 nTPM
Bronchus	245.6 nTPM

Basal squamous epithelial cells	23.3 nTPM
Esophagus	23.3 nTPM

Undifferentiated cells	149.1 nTPM
Colon	195.2 nTPM
Rectum	126.3 nTPM
Small intestine	125.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC