Single cell type - ACAT2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ACAT2
Gene descriptionⁱ Full gene name according to HGNC.	Acetyl-CoA acetyltransferase 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Respiratory epithelial cells - Mucosal defense (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Basal respiratory cells, Hepatocytes, Early spermatids, Exocrine glandular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Basal respiratory cells, Hepatocytes, Early spermatids, Exocrine glandular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ACAT2 is part of cluster 26 Respiratory epithelial cells - Mucosal defense with confidenceⁱ 1
293 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
FDFT1	Farnesyl-diphosphate farnesyltransferase 1	0.7339	26
EBP	EBP cholestenol delta-isomerase	0.7275	15
NSDHL	NAD(P) dependent steroid dehydrogenase-like	0.7263	26
MMAB	Metabolism of cobalamin associated B	0.7235	15
FDPS	Farnesyl diphosphate synthase	0.7130	26
DHCR7	7-dehydrocholesterol reductase	0.6765	15
MSMO1	Methylsterol monooxygenase 1	0.6378	15
SERPINB4	Serpin family B member 4	0.6290	26
SERPINB3	Serpin family B member 3	0.6288	26
PGLYRP4	Peptidoglycan recognition protein 4	0.6284	26
SPRR2D	Small proline rich protein 2D	0.6267	26
CXCL1	C-X-C motif chemokine ligand 1	0.6237	26
IL36G	Interleukin 36 gamma	0.6235	26
CCL20	C-C motif chemokine ligand 20	0.6219	26
TMEM45A	Transmembrane protein 45A	0.6204	26

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	11.8 nTPM
Breast	15.0 nTPM
Adipose tissue	8.5 nTPM

Alveolar cells type 1	12.8 nTPM
Lung	12.8 nTPM

Alveolar cells type 2	45.4 nTPM
Lung	45.4 nTPM

Astrocytes	15.3 nTPM
Brain	15.3 nTPM

B-cells	25.8 nTPM
Liver	59.9 nTPM
Colon	28.7 nTPM
PBMC	27.1 nTPM
Lymph node	25.3 nTPM
Spleen	23.9 nTPM
Kidney	23.8 nTPM
Bone marrow	22.9 nTPM
Stomach	20.4 nTPM
Adipose tissue	15.0 nTPM
Breast	10.7 nTPM

Dendritic cells	28.6 nTPM
Breast	38.6 nTPM
PBMC	23.9 nTPM
Adipose tissue	23.4 nTPM

Endothelial cells	18.8 nTPM
Placenta	79.5 nTPM
Ovary	48.6 nTPM
Lung	18.7 nTPM
Prostate	15.3 nTPM
Liver	14.7 nTPM
Breast	13.8 nTPM
Testis	13.0 nTPM
Eye	12.9 nTPM
Skin	11.5 nTPM
Endometrium	9.1 nTPM
Esophagus	8.8 nTPM
Skeletal muscle	8.1 nTPM
Pancreas	7.7 nTPM
Heart muscle	1.3 nTPM

Fibroblasts	21.6 nTPM
Placenta	67.1 nTPM
Ovary	38.4 nTPM
Prostate	24.7 nTPM
Skin	24.2 nTPM
Breast	18.7 nTPM
Skeletal muscle	18.4 nTPM
Esophagus	17.0 nTPM
Adipose tissue	13.9 nTPM
Lung	7.8 nTPM
Stomach	6.0 nTPM
Heart muscle	1.3 nTPM

Intestinal goblet cells	23.6 nTPM
Colon	25.7 nTPM
Small intestine	24.0 nTPM
Rectum	21.0 nTPM

Macrophages	25.7 nTPM
Prostate	73.3 nTPM
Ovary	65.4 nTPM
Pancreas	51.0 nTPM
Breast	36.8 nTPM
Endometrium	26.3 nTPM
Skeletal muscle	21.7 nTPM
Adipose tissue	19.7 nTPM
Spleen	18.6 nTPM
Lung	18.5 nTPM
Esophagus	17.3 nTPM
Testis	13.4 nTPM
Kidney	10.6 nTPM
Bone marrow	7.5 nTPM
Stomach	5.9 nTPM
PBMC	0.0 nTPM

Paneth cells	28.8 nTPM
Colon	56.0 nTPM
Rectum	19.3 nTPM
Small intestine	11.3 nTPM

Plasma cells	25.5 nTPM
Spleen	60.5 nTPM
Bone marrow	36.2 nTPM
Stomach	13.1 nTPM
Adipose tissue	11.6 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	30.1 nTPM
Bronchus	38.3 nTPM
Lung	21.9 nTPM

Smooth muscle cells	44.9 nTPM
Pancreas	132.1 nTPM
Bronchus	105.3 nTPM
Ovary	89.3 nTPM
Skin	38.0 nTPM
Breast	31.3 nTPM
Esophagus	29.4 nTPM
Prostate	25.9 nTPM
Endometrium	17.0 nTPM
Adipose tissue	11.8 nTPM
Skeletal muscle	11.3 nTPM
Heart muscle	3.0 nTPM

T-cells	31.0 nTPM
Ovary	48.9 nTPM
Prostate	47.3 nTPM
Kidney	40.4 nTPM
Breast	35.5 nTPM
Lymph node	33.3 nTPM
Stomach	33.0 nTPM
Esophagus	32.8 nTPM
PBMC	30.1 nTPM
Skin	29.5 nTPM
Liver	29.2 nTPM
Colon	28.0 nTPM
Adipose tissue	25.2 nTPM
Endometrium	24.7 nTPM
Lung	22.8 nTPM
Spleen	22.8 nTPM
Bone marrow	22.3 nTPM
Skeletal muscle	21.4 nTPM

Basal keratinocytes	26.1 nTPM
Skin	26.1 nTPM

Basal prostatic cells	32.4 nTPM
Prostate	32.4 nTPM

Cholangiocytes	42.9 nTPM
Liver	42.9 nTPM

Collecting duct cells	20.2 nTPM
Kidney	20.2 nTPM

Undifferentiated cells	33.0 nTPM
Colon	46.9 nTPM
Small intestine	27.6 nTPM
Rectum	24.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC