Single cell type - AQP3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AQP3
Gene descriptionⁱ Full gene name according to HGNC.	Aquaporin 3 (Gill blood group)
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Blood group antigen proteins Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Epithelial cell types - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Suprabasal keratinocytes, Urothelial cells, Squamous epithelial cells, Basal keratinocytes, Collecting duct cells, Prostatic glandular cells, Basal prostatic cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (T-reg, MAIT T-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HaCaT, HeLa, JURKAT, RT4, SK-BR-3, U-266/70, U-266/84)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Suprabasal keratinocytes, Urothelial cells, Squamous epithelial cells, Basal keratinocytes, Collecting duct cells, Prostatic glandular cells, Basal prostatic cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AQP3 is part of cluster 22 Epithelial cell types - Mixed function with confidenceⁱ 1
298 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
FOXN1	Forkhead box N1	0.9069	61
GRHL3	Grainyhead like transcription factor 3	0.8736	61
KRTAP3-2	Keratin associated protein 3-2	0.8459	61
SERPINB11	Serpin family B member 11	0.8187	61
RHOV	Ras homolog family member V	0.8180	61
BNIPL	BCL2 interacting protein like	0.8035	61
MUC21	Mucin 21, cell surface associated	0.8024	61
WNT7B	Wnt family member 7B	0.7984	22
PRRG4	Proline rich and Gla domain 4	0.7919	22
GJB3	Gap junction protein beta 3	0.7871	22
PADI3	Peptidyl arginine deiminase 3	0.7797	22
IVL	Involucrin	0.7574	61
SULT2B1	Sulfotransferase family 2B member 1	0.7531	22
GAN	Gigaxonin	0.7513	22
BARX2	BARX homeobox 2	0.7451	61

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	9.2 nTPM
Breast	12.3 nTPM
Adipose tissue	6.2 nTPM

Alveolar cells type 1	38.5 nTPM
Lung	38.5 nTPM

Alveolar cells type 2	249.9 nTPM
Lung	249.9 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	42.4 nTPM
Liver	296.6 nTPM
Breast	89.6 nTPM
PBMC	11.5 nTPM
Lymph node	7.1 nTPM
Adipose tissue	6.6 nTPM
Stomach	5.9 nTPM
Kidney	4.8 nTPM
Spleen	2.4 nTPM
Colon	0.0 nTPM
Bone marrow	0.0 nTPM

Basal squamous epithelial cells	811.0 nTPM
Esophagus	811.0 nTPM

Dendritic cells	21.6 nTPM
PBMC	27.9 nTPM
Breast	23.6 nTPM
Adipose tissue	13.4 nTPM

Endothelial cells	37.1 nTPM
Skin	125.0 nTPM
Prostate	95.8 nTPM
Heart muscle	78.9 nTPM
Esophagus	61.5 nTPM
Liver	42.2 nTPM
Ovary	30.0 nTPM
Eye	20.3 nTPM
Skeletal muscle	16.3 nTPM
Endometrium	13.8 nTPM
Placenta	13.7 nTPM
Breast	9.6 nTPM
Lung	9.3 nTPM
Testis	2.4 nTPM
Pancreas	0.0 nTPM

Fibroblasts	27.5 nTPM
Prostate	84.0 nTPM
Skin	77.8 nTPM
Placenta	52.1 nTPM
Stomach	20.8 nTPM
Esophagus	19.4 nTPM
Heart muscle	13.5 nTPM
Lung	12.3 nTPM
Adipose tissue	9.0 nTPM
Breast	8.8 nTPM
Skeletal muscle	4.0 nTPM
Ovary	1.2 nTPM

Intestinal goblet cells	104.0 nTPM
Colon	137.2 nTPM
Small intestine	134.4 nTPM
Rectum	40.5 nTPM

Macrophages	15.3 nTPM
Lung	101.1 nTPM
Prostate	38.9 nTPM
Breast	27.8 nTPM
Pancreas	17.8 nTPM
Esophagus	12.6 nTPM
Endometrium	11.6 nTPM
Stomach	5.9 nTPM
Adipose tissue	3.9 nTPM
Kidney	3.8 nTPM
Ovary	2.8 nTPM
Skeletal muscle	2.7 nTPM
Spleen	1.0 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Bone marrow	0.0 nTPM

Paneth cells	8.1 nTPM
Small intestine	18.8 nTPM
Colon	3.3 nTPM
Rectum	2.4 nTPM

Plasma cells	246.9 nTPM
Spleen	409.3 nTPM
Bone marrow	370.8 nTPM
Esophagus	203.2 nTPM
Stomach	173.9 nTPM
Adipose tissue	77.4 nTPM

Respiratory ciliated cells	546.0 nTPM
Bronchus	943.3 nTPM
Lung	148.6 nTPM

Smooth muscle cells	35.8 nTPM
Bronchus	169.1 nTPM
Prostate	78.8 nTPM
Skin	51.3 nTPM
Esophagus	24.8 nTPM
Heart muscle	20.2 nTPM
Ovary	16.5 nTPM
Breast	11.0 nTPM
Endometrium	7.4 nTPM
Adipose tissue	5.8 nTPM
Pancreas	4.5 nTPM
Skeletal muscle	4.3 nTPM

Cholangiocytes	157.3 nTPM
Liver	157.3 nTPM

Cone photoreceptor cells	2.1 nTPM
Eye	2.1 nTPM

Cytotrophoblasts	0.9 nTPM
Placenta	0.9 nTPM

Early spermatids	2.2 nTPM
Testis	2.2 nTPM

T-cells	73.4 nTPM
Breast	128.4 nTPM
Skin	125.7 nTPM
PBMC	112.6 nTPM
Lymph node	109.8 nTPM
Prostate	94.7 nTPM
Adipose tissue	91.3 nTPM
Spleen	81.0 nTPM
Ovary	75.7 nTPM
Esophagus	58.9 nTPM
Lung	54.6 nTPM
Bone marrow	48.3 nTPM
Stomach	47.4 nTPM
Skeletal muscle	47.0 nTPM
Kidney	46.5 nTPM
Liver	43.8 nTPM
Colon	43.7 nTPM
Endometrium	37.7 nTPM

Undifferentiated cells	7.3 nTPM
Small intestine	15.0 nTPM
Colon	5.0 nTPM
Rectum	1.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC