Single cell type - PRRG4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PRRG4
Gene descriptionⁱ Full gene name according to HGNC.	Proline rich and Gla domain 4
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Epithelial cell types - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Urothelial cells, Prostatic glandular cells, Basal squamous epithelial cells, Squamous epithelial cells, Suprabasal keratinocytes, Basal keratinocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (neutrophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HaCaT, hTCEpi, SK-BR-3)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Urothelial cells, Prostatic glandular cells, Basal squamous epithelial cells, Squamous epithelial cells, Suprabasal keratinocytes, Basal keratinocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PRRG4 is part of cluster 22 Epithelial cell types - Mixed function with confidenceⁱ 1
298 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
AQP3	Aquaporin 3 (Gill blood group)	0.7919	22
MPZL2	Myelin protein zero like 2	0.7796	22
KLF5	Kruppel like factor 5	0.7636	22
GRB7	Growth factor receptor bound protein 7	0.7450	61
CLDN1	Claudin 1	0.7417	22
WNT7B	Wnt family member 7B	0.7235	22
FOXN1	Forkhead box N1	0.7176	61
C15orf62	Chromosome 15 open reading frame 62	0.7147	22
KRTAP3-2	Keratin associated protein 3-2	0.7138	61
RHOV	Ras homolog family member V	0.7099	61
BARX2	BARX homeobox 2	0.7055	61
GJB3	Gap junction protein beta 3	0.6975	22
TACSTD2	Tumor associated calcium signal transducer 2	0.6928	22
CLDN4	Claudin 4	0.6875	22
SERPINB11	Serpin family B member 11	0.6846	61

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	4.2 nTPM
Adipose tissue	5.6 nTPM
Breast	2.7 nTPM

Alveolar cells type 1	28.9 nTPM
Lung	28.9 nTPM

Alveolar cells type 2	23.0 nTPM
Lung	23.0 nTPM

Astrocytes	1.0 nTPM
Brain	1.0 nTPM

B-cells	2.8 nTPM
Colon	11.2 nTPM
Kidney	4.8 nTPM
Breast	4.0 nTPM
Liver	3.3 nTPM
Adipose tissue	1.5 nTPM
Lymph node	1.0 nTPM
Bone marrow	0.8 nTPM
Spleen	0.7 nTPM
Stomach	0.5 nTPM
PBMC	0.0 nTPM

Basal squamous epithelial cells	145.1 nTPM
Esophagus	145.1 nTPM

Dendritic cells	1.7 nTPM
Breast	3.3 nTPM
Adipose tissue	1.7 nTPM
PBMC	0.0 nTPM

Endothelial cells	10.2 nTPM
Skin	20.2 nTPM
Prostate	19.2 nTPM
Skeletal muscle	17.9 nTPM
Pancreas	15.5 nTPM
Endometrium	12.5 nTPM
Testis	10.6 nTPM
Liver	10.1 nTPM
Esophagus	9.7 nTPM
Breast	9.7 nTPM
Lung	6.2 nTPM
Heart muscle	5.6 nTPM
Eye	3.3 nTPM
Placenta	2.2 nTPM
Ovary	0.2 nTPM

Fibroblasts	3.6 nTPM
Prostate	15.8 nTPM
Skin	7.8 nTPM
Lung	4.5 nTPM
Breast	2.2 nTPM
Placenta	2.1 nTPM
Skeletal muscle	1.9 nTPM
Stomach	1.5 nTPM
Esophagus	1.4 nTPM
Ovary	1.3 nTPM
Heart muscle	0.6 nTPM
Adipose tissue	0.4 nTPM

Intestinal goblet cells	34.7 nTPM
Colon	50.7 nTPM
Small intestine	27.5 nTPM
Rectum	26.0 nTPM

Macrophages	12.6 nTPM
Prostate	37.9 nTPM
Bone marrow	20.0 nTPM
Spleen	19.7 nTPM
Kidney	18.1 nTPM
Ovary	16.8 nTPM
Endometrium	13.3 nTPM
Skeletal muscle	11.6 nTPM
Adipose tissue	9.6 nTPM
Stomach	8.8 nTPM
Esophagus	7.9 nTPM
Lung	7.8 nTPM
Breast	7.7 nTPM
Testis	7.5 nTPM
Pancreas	2.5 nTPM
PBMC	0.0 nTPM

Paneth cells	19.5 nTPM
Rectum	27.2 nTPM
Colon	16.5 nTPM
Small intestine	15.0 nTPM

Plasma cells	6.9 nTPM
Esophagus	18.4 nTPM
Stomach	9.2 nTPM
Spleen	4.8 nTPM
Adipose tissue	1.9 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	51.1 nTPM
Bronchus	70.1 nTPM
Lung	32.1 nTPM

Smooth muscle cells	8.8 nTPM
Skeletal muscle	36.0 nTPM
Heart muscle	18.1 nTPM
Prostate	16.2 nTPM
Esophagus	7.8 nTPM
Bronchus	7.5 nTPM
Skin	5.3 nTPM
Endometrium	1.8 nTPM
Pancreas	1.6 nTPM
Ovary	1.2 nTPM
Adipose tissue	0.9 nTPM
Breast	0.5 nTPM

Cholangiocytes	95.8 nTPM
Liver	95.8 nTPM

Collecting duct cells	47.8 nTPM
Kidney	47.8 nTPM

Cytotrophoblasts	24.4 nTPM
Placenta	24.4 nTPM

Distal enterocytes	46.8 nTPM
Colon	50.0 nTPM
Rectum	43.6 nTPM

T-cells	2.6 nTPM
Prostate	9.5 nTPM
Endometrium	6.9 nTPM
Esophagus	6.6 nTPM
Lung	3.0 nTPM
Colon	2.9 nTPM
Skin	2.8 nTPM
Skeletal muscle	2.5 nTPM
Breast	2.3 nTPM
Liver	1.9 nTPM
Kidney	1.4 nTPM
Adipose tissue	0.9 nTPM
Stomach	0.9 nTPM
Spleen	0.8 nTPM
Lymph node	0.5 nTPM
PBMC	0.4 nTPM
Bone marrow	0.3 nTPM
Ovary	0.0 nTPM

Undifferentiated cells	47.0 nTPM
Colon	58.1 nTPM
Small intestine	43.3 nTPM
Rectum	39.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC