Single cell type - NINJ2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NINJ2
Gene descriptionⁱ Full gene name according to HGNC.	Ninjurin 2
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Adipocytes & Endothelial cells - Angiogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Oligodendrocytes, Alveolar cells type 2, Kupffer cells, Erythroid cells, Hofbauer cells, Adipocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HEL, MOLT-4, THP-1, U-698)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Oligodendrocytes, Alveolar cells type 2, Kupffer cells, Erythroid cells, Hofbauer cells, Adipocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NINJ2 is part of cluster 14 Adipocytes & Endothelial cells - Angiogenesis with confidenceⁱ 1
354 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ITM2A	Integral membrane protein 2A	0.5640	14
ICAM4	Intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)	0.5365	11
CMPK2	Cytidine/uridine monophosphate kinase 2	0.5187	6
EVI2A	Ecotropic viral integration site 2A	0.5078	19
SLC48A1	Solute carrier family 48 member 1	0.5057	6
STAT2	Signal transducer and activator of transcription 2	0.4917	59
FMO2	Flavin containing dimethylaniline monoxygenase 2	0.4896	14
SAMHD1	SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1	0.4880	19
ENPP2	Ectonucleotide pyrophosphatase/phosphodiesterase 2	0.4811	42
SHROOM4	Shroom family member 4	0.4810	42
CAT	Catalase	0.4785	6
IFIT3	Interferon induced protein with tetratricopeptide repeats 3	0.4771	14
S100A4	S100 calcium binding protein A4	0.4665	19
ADGRF5	Adhesion G protein-coupled receptor F5	0.4649	14
PIP4K2A	Phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	0.4624	42

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Alveolar cells type 1	22.4 nTPM
Lung	22.4 nTPM

Astrocytes	0.3 nTPM
Brain	0.3 nTPM

B-cells	5.3 nTPM
Kidney	14.3 nTPM
Liver	14.2 nTPM
Bone marrow	4.9 nTPM
Breast	4.0 nTPM
Adipose tissue	3.5 nTPM
Lymph node	3.1 nTPM
Spleen	2.8 nTPM
Stomach	2.7 nTPM
Colon	1.6 nTPM
PBMC	1.4 nTPM

Basal keratinocytes	5.7 nTPM
Skin	5.7 nTPM

Basal prostatic cells	0.3 nTPM
Prostate	0.3 nTPM

Dendritic cells	8.9 nTPM
PBMC	12.0 nTPM
Breast	9.9 nTPM
Adipose tissue	4.9 nTPM

Endothelial cells	9.4 nTPM
Skeletal muscle	29.4 nTPM
Heart muscle	18.3 nTPM
Breast	15.5 nTPM
Skin	14.7 nTPM
Liver	12.8 nTPM
Esophagus	10.9 nTPM
Lung	6.2 nTPM
Prostate	6.2 nTPM
Placenta	5.7 nTPM
Ovary	4.3 nTPM
Testis	4.1 nTPM
Endometrium	1.8 nTPM
Eye	1.6 nTPM
Pancreas	0.0 nTPM

Fibroblasts	13.5 nTPM
Adipose tissue	34.1 nTPM
Skeletal muscle	27.3 nTPM
Heart muscle	25.3 nTPM
Skin	19.1 nTPM
Breast	15.2 nTPM
Esophagus	11.8 nTPM
Prostate	6.3 nTPM
Stomach	6.0 nTPM
Placenta	2.3 nTPM
Lung	1.2 nTPM
Ovary	0.1 nTPM

Intestinal goblet cells	3.5 nTPM
Rectum	5.8 nTPM
Small intestine	2.4 nTPM
Colon	2.2 nTPM

Macrophages	18.0 nTPM
Prostate	37.9 nTPM
Kidney	30.3 nTPM
Stomach	23.5 nTPM
Testis	23.2 nTPM
Ovary	22.0 nTPM
Pancreas	20.4 nTPM
Spleen	18.6 nTPM
Breast	18.4 nTPM
Esophagus	17.3 nTPM
Skeletal muscle	14.6 nTPM
Lung	13.2 nTPM
Bone marrow	12.5 nTPM
Endometrium	10.9 nTPM
Adipose tissue	7.1 nTPM
PBMC	0.0 nTPM

Paneth cells	2.6 nTPM
Rectum	4.2 nTPM
Small intestine	3.7 nTPM
Colon	0.0 nTPM

Plasma cells	8.1 nTPM
Bone marrow	11.5 nTPM
Stomach	9.6 nTPM
Spleen	8.8 nTPM
Esophagus	6.1 nTPM
Adipose tissue	4.5 nTPM

Respiratory ciliated cells	5.0 nTPM
Lung	7.3 nTPM
Bronchus	2.7 nTPM

Smooth muscle cells	1.6 nTPM
Pancreas	5.3 nTPM
Adipose tissue	5.2 nTPM
Skeletal muscle	2.4 nTPM
Skin	1.7 nTPM
Breast	1.4 nTPM
Heart muscle	0.6 nTPM
Prostate	0.6 nTPM
Ovary	0.4 nTPM
Endometrium	0.1 nTPM
Bronchus	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	12.5 nTPM
Skin	27.6 nTPM
Kidney	21.2 nTPM
Liver	20.9 nTPM
Prostate	19.0 nTPM
Lung	15.2 nTPM
Stomach	12.9 nTPM
Breast	12.4 nTPM
Bone marrow	11.5 nTPM
PBMC	10.7 nTPM
Skeletal muscle	10.2 nTPM
Spleen	9.3 nTPM
Lymph node	8.9 nTPM
Colon	8.5 nTPM
Adipose tissue	7.1 nTPM
Ovary	6.7 nTPM
Esophagus	6.6 nTPM
Endometrium	3.6 nTPM

Cholangiocytes	1.5 nTPM
Liver	1.5 nTPM

Distal enterocytes	2.1 nTPM
Rectum	2.1 nTPM
Colon	2.0 nTPM

Distal tubular cells	1.9 nTPM
Kidney	1.9 nTPM

Undifferentiated cells	3.1 nTPM
Small intestine	4.6 nTPM
Rectum	4.0 nTPM
Colon	0.6 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC