Single cell type - PLEKHF2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PLEKHF2
Gene descriptionⁱ Full gene name according to HGNC.	Pleckstrin homology and FYVE domain containing 2
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	B-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Daudi, U-698)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PLEKHF2 is part of cluster 57 B-cells - Immune response with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TSTD1	Thiosulfate sulfurtransferase like domain containing 1	0.5505	53
GPBP1	GC-rich promoter binding protein 1	0.5183	22
BCL10	BCL10 immune signaling adaptor	0.5052	22
TNFRSF13C	TNF receptor superfamily member 13C	0.4999	57
METTL21A	Methyltransferase like 21A	0.4888	22
FCER2	Fc fragment of IgE receptor II	0.4871	57
HLA-DOB	Major histocompatibility complex, class II, DO beta	0.4846	57
VPREB3	V-set pre-B cell surrogate light chain 3	0.4821	57
CD72	CD72 molecule	0.4778	57
FCRLA	Fc receptor like A	0.4773	57
TMEM154	Transmembrane protein 154	0.4771	57
CD19	CD19 molecule	0.4748	57
NFKB2	Nuclear factor kappa B subunit 2	0.4744	41
BANK1	B cell scaffold protein with ankyrin repeats 1	0.4743	57
BTG1	BTG anti-proliferation factor 1	0.4736	24

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	29.3 nTPM
Breast	30.3 nTPM
Adipose tissue	28.3 nTPM

Alveolar cells type 1	12.8 nTPM
Lung	12.8 nTPM

Alveolar cells type 2	14.1 nTPM
Lung	14.1 nTPM

Astrocytes	7.2 nTPM
Brain	7.2 nTPM

Basal keratinocytes	61.4 nTPM
Skin	61.4 nTPM

B-cells	88.8 nTPM
PBMC	188.4 nTPM
Bone marrow	135.7 nTPM
Kidney	114.3 nTPM
Stomach	84.5 nTPM
Spleen	79.3 nTPM
Adipose tissue	74.2 nTPM
Lymph node	74.1 nTPM
Colon	60.7 nTPM
Breast	40.2 nTPM
Liver	36.5 nTPM

Dendritic cells	17.8 nTPM
Adipose tissue	23.8 nTPM
Breast	17.5 nTPM
PBMC	12.0 nTPM

Endothelial cells	18.5 nTPM
Skin	32.4 nTPM
Breast	26.5 nTPM
Skeletal muscle	25.7 nTPM
Heart muscle	23.0 nTPM
Esophagus	22.8 nTPM
Prostate	22.3 nTPM
Endometrium	18.8 nTPM
Liver	18.3 nTPM
Ovary	13.3 nTPM
Lung	12.5 nTPM
Placenta	12.5 nTPM
Testis	11.9 nTPM
Eye	11.3 nTPM
Pancreas	7.7 nTPM

Fibroblasts	11.9 nTPM
Skin	22.3 nTPM
Stomach	20.8 nTPM
Prostate	17.9 nTPM
Esophagus	14.1 nTPM
Adipose tissue	12.1 nTPM
Breast	11.7 nTPM
Skeletal muscle	10.2 nTPM
Placenta	8.8 nTPM
Ovary	6.6 nTPM
Lung	3.4 nTPM
Heart muscle	2.8 nTPM

Intestinal goblet cells	14.6 nTPM
Colon	16.5 nTPM
Rectum	15.9 nTPM
Small intestine	11.4 nTPM

Macrophages	34.7 nTPM
Adipose tissue	74.5 nTPM
PBMC	68.2 nTPM
Prostate	65.4 nTPM
Kidney	34.8 nTPM
Pancreas	33.2 nTPM
Stomach	29.4 nTPM
Breast	29.1 nTPM
Spleen	29.0 nTPM
Ovary	27.4 nTPM
Esophagus	25.2 nTPM
Testis	24.4 nTPM
Skeletal muscle	23.3 nTPM
Endometrium	22.5 nTPM
Bone marrow	22.5 nTPM
Lung	11.8 nTPM

Paneth cells	39.0 nTPM
Colon	69.2 nTPM
Rectum	25.4 nTPM
Small intestine	22.5 nTPM

Plasma cells	21.3 nTPM
Adipose tissue	38.0 nTPM
Bone marrow	23.0 nTPM
Spleen	23.0 nTPM
Stomach	16.5 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	26.2 nTPM
Bronchus	30.5 nTPM
Lung	21.9 nTPM

Smooth muscle cells	14.5 nTPM
Adipose tissue	25.5 nTPM
Esophagus	21.7 nTPM
Skin	18.8 nTPM
Prostate	18.1 nTPM
Endometrium	17.8 nTPM
Breast	14.1 nTPM
Skeletal muscle	12.0 nTPM
Ovary	11.2 nTPM
Bronchus	9.5 nTPM
Pancreas	6.9 nTPM
Heart muscle	4.2 nTPM

Cholangiocytes	31.5 nTPM
Liver	31.5 nTPM

Collecting duct cells	5.5 nTPM
Kidney	5.5 nTPM

Cytotrophoblasts	51.6 nTPM
Placenta	51.6 nTPM

Distal enterocytes	46.7 nTPM
Colon	52.4 nTPM
Rectum	40.9 nTPM

T-cells	27.4 nTPM
Prostate	94.7 nTPM
Adipose tissue	39.5 nTPM
Stomach	31.3 nTPM
Skin	29.9 nTPM
Ovary	26.7 nTPM
Lymph node	26.4 nTPM
Esophagus	26.2 nTPM
Colon	25.6 nTPM
Spleen	24.9 nTPM
Bone marrow	23.3 nTPM
Kidney	22.6 nTPM
PBMC	21.2 nTPM
Endometrium	18.9 nTPM
Breast	18.7 nTPM
Liver	17.3 nTPM
Skeletal muscle	12.9 nTPM
Lung	6.1 nTPM

Undifferentiated cells	17.7 nTPM
Colon	23.5 nTPM
Rectum	15.6 nTPM
Small intestine	14.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC