Single cell type - P4HTM - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	P4HTM
Gene descriptionⁱ Full gene name according to HGNC.	Prolyl 4-hydroxylase, transmembrane
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Ciliated cells - Cilium organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Respiratory ciliated cells, Late spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Respiratory ciliated cells, Late spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

P4HTM is part of cluster 49 Ciliated cells - Cilium organization with confidenceⁱ 1
457 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
LRP11	LDL receptor related protein 11	0.6935	49
CAPSL	Calcyphosine like	0.6528	49
EFHC1	EF-hand domain containing 1	0.6343	49
DNAH9	Dynein axonemal heavy chain 9	0.6343	49
FAM227A	Family with sequence similarity 227 member A	0.6292	49
CDS1	CDP-diacylglycerol synthase 1	0.6289	49
DCDC1	Doublecortin domain containing 1	0.6253	49
DNAJB13	DnaJ heat shock protein family (Hsp40) member B13	0.6198	49
C20orf85	Chromosome 20 open reading frame 85	0.6171	49
CLDN9	Claudin 9	0.6159	49
TMEM190	Transmembrane protein 190	0.6058	49
C9orf116	Chromosome 9 open reading frame 116	0.5977	49
PIFO	Primary cilia formation	0.5976	49
FAM47E	Family with sequence similarity 47 member E	0.5836	49
SPAG1	Sperm associated antigen 1	0.5833	49

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	15.1 nTPM
Adipose tissue	15.5 nTPM
Breast	14.8 nTPM

Alveolar cells type 1	22.4 nTPM
Lung	22.4 nTPM

Alveolar cells type 2	17.3 nTPM
Lung	17.3 nTPM

Astrocytes	36.2 nTPM
Brain	36.2 nTPM

B-cells	16.1 nTPM
Breast	22.8 nTPM
Liver	18.0 nTPM
Lymph node	17.7 nTPM
Bone marrow	16.4 nTPM
Stomach	15.5 nTPM
Spleen	15.3 nTPM
Colon	14.4 nTPM
Kidney	14.3 nTPM
PBMC	14.3 nTPM
Adipose tissue	12.6 nTPM

Dendritic cells	13.4 nTPM
Breast	26.6 nTPM
Adipose tissue	13.5 nTPM
PBMC	0.0 nTPM

Endothelial cells	12.6 nTPM
Eye	26.7 nTPM
Liver	19.2 nTPM
Breast	16.6 nTPM
Skin	15.4 nTPM
Placenta	15.3 nTPM
Prostate	13.7 nTPM
Testis	13.6 nTPM
Esophagus	13.4 nTPM
Endometrium	12.8 nTPM
Skeletal muscle	11.1 nTPM
Ovary	8.5 nTPM
Heart muscle	6.2 nTPM
Pancreas	3.9 nTPM
Lung	0.0 nTPM

Fibroblasts	28.1 nTPM
Adipose tissue	40.5 nTPM
Prostate	38.8 nTPM
Esophagus	34.1 nTPM
Breast	32.1 nTPM
Skeletal muscle	30.3 nTPM
Stomach	29.7 nTPM
Skin	29.6 nTPM
Placenta	28.2 nTPM
Ovary	18.4 nTPM
Heart muscle	16.6 nTPM
Lung	11.1 nTPM

Intestinal goblet cells	40.4 nTPM
Small intestine	51.8 nTPM
Colon	36.4 nTPM
Rectum	33.1 nTPM

Macrophages	13.0 nTPM
Stomach	29.4 nTPM
Pancreas	25.5 nTPM
Ovary	19.5 nTPM
Breast	16.0 nTPM
Kidney	12.0 nTPM
Adipose tissue	12.0 nTPM
Prostate	11.5 nTPM
Endometrium	11.3 nTPM
Lung	11.2 nTPM
Skeletal muscle	11.0 nTPM
Esophagus	9.4 nTPM
Testis	8.8 nTPM
Bone marrow	8.7 nTPM
Spleen	8.3 nTPM
PBMC	0.0 nTPM

Paneth cells	51.8 nTPM
Small intestine	67.5 nTPM
Colon	46.1 nTPM
Rectum	41.9 nTPM

Plasma cells	28.9 nTPM
Esophagus	49.4 nTPM
Stomach	29.2 nTPM
Bone marrow	26.4 nTPM
Spleen	21.5 nTPM
Adipose tissue	18.1 nTPM

Respiratory ciliated cells	169.4 nTPM
Bronchus	255.6 nTPM
Lung	83.1 nTPM

Smooth muscle cells	24.7 nTPM
Prostate	35.3 nTPM
Ovary	35.1 nTPM
Skin	32.1 nTPM
Breast	30.9 nTPM
Bronchus	30.7 nTPM
Esophagus	29.4 nTPM
Adipose tissue	22.0 nTPM
Endometrium	21.3 nTPM
Skeletal muscle	16.9 nTPM
Pancreas	11.7 nTPM
Heart muscle	6.8 nTPM

T-cells	25.0 nTPM
Ovary	53.4 nTPM
Breast	34.2 nTPM
Spleen	30.1 nTPM
Kidney	28.7 nTPM
Skin	28.5 nTPM
Stomach	28.5 nTPM
Prostate	28.4 nTPM
Lymph node	23.8 nTPM
Colon	23.3 nTPM
Bone marrow	23.1 nTPM
Adipose tissue	21.8 nTPM
Liver	20.9 nTPM
PBMC	19.6 nTPM
Endometrium	18.0 nTPM
Skeletal muscle	17.4 nTPM
Esophagus	13.1 nTPM
Lung	12.2 nTPM

Basal keratinocytes	9.2 nTPM
Skin	9.2 nTPM

Basal prostatic cells	34.6 nTPM
Prostate	34.6 nTPM

Basal respiratory cells	41.1 nTPM
Bronchus	41.1 nTPM

Basal squamous epithelial cells	12.5 nTPM
Esophagus	12.5 nTPM

Undifferentiated cells	40.9 nTPM
Rectum	50.5 nTPM
Small intestine	39.1 nTPM
Colon	33.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC