Single cell type - ASPH - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ASPH
Gene descriptionⁱ Full gene name according to HGNC.	Aspartate beta-hydroxylase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Enzymes Human disease related genes Metabolic proteins Plasma proteins Potential drug targets Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	21

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Muller glia cells - Visual perception (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Muller glia cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (plasmacytoid DC)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (U-87 MG)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Muller glia cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ASPH is part of cluster 5 Muller glia cells - Visual perception with confidenceⁱ 1
155 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
F3	Coagulation factor III, tissue factor	0.6811	16
ITGAV	Integrin subunit alpha V	0.5859	44
LAMC2	Laminin subunit gamma 2	0.5789	16
KRT39	Keratin 39	0.5728	5
TRH	Thyrotropin releasing hormone	0.5638	5
RTN4	Reticulon 4	0.5568	5
ADAM9	ADAM metallopeptidase domain 9	0.5422	16
CA14	Carbonic anhydrase 14	0.5408	5
ANXA2	Annexin A2	0.5387	16
KPNA7	Karyopherin subunit alpha 7	0.5265	16
WIF1	WNT inhibitory factor 1	0.5198	5
ANXA3	Annexin A3	0.5182	16
S100A6	S100 calcium binding protein A6	0.5149	16
CRABP1	Cellular retinoic acid binding protein 1	0.5143	5
PLAU	Plasminogen activator, urokinase	0.5137	22

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	79.1 nTPM
Adipose tissue	90.8 nTPM
Breast	67.4 nTPM

Alveolar cells type 1	195.5 nTPM
Lung	195.5 nTPM

Alveolar cells type 2	244.0 nTPM
Lung	244.0 nTPM

Astrocytes	366.6 nTPM
Brain	366.6 nTPM

B-cells	13.8 nTPM
Breast	21.3 nTPM
Colon	19.2 nTPM
Bone marrow	15.5 nTPM
Stomach	15.5 nTPM
Lymph node	14.0 nTPM
Adipose tissue	12.6 nTPM
Spleen	12.5 nTPM
PBMC	9.9 nTPM
Kidney	9.5 nTPM
Liver	8.0 nTPM

Dendritic cells	40.3 nTPM
PBMC	103.6 nTPM
Adipose tissue	9.7 nTPM
Breast	7.6 nTPM

Endothelial cells	85.9 nTPM
Prostate	280.5 nTPM
Lung	118.5 nTPM
Heart muscle	100.6 nTPM
Endometrium	82.6 nTPM
Ovary	81.3 nTPM
Skin	78.6 nTPM
Breast	74.4 nTPM
Pancreas	65.6 nTPM
Skeletal muscle	62.3 nTPM
Esophagus	60.3 nTPM
Eye	55.8 nTPM
Testis	54.8 nTPM
Liver	46.7 nTPM
Placenta	40.9 nTPM

Enteroendocrine cells	116.5 nTPM
Colon	145.7 nTPM
Rectum	87.4 nTPM

Fibroblasts	132.0 nTPM
Adipose tissue	249.7 nTPM
Heart muscle	198.4 nTPM
Skeletal muscle	183.5 nTPM
Breast	143.1 nTPM
Esophagus	134.9 nTPM
Lung	130.7 nTPM
Skin	112.6 nTPM
Stomach	93.6 nTPM
Prostate	71.8 nTPM
Ovary	69.1 nTPM
Placenta	64.9 nTPM

Intestinal goblet cells	215.1 nTPM
Colon	308.1 nTPM
Rectum	219.8 nTPM
Small intestine	117.5 nTPM

Macrophages	48.1 nTPM
Stomach	114.3 nTPM
Skeletal muscle	71.5 nTPM
Lung	68.3 nTPM
Pancreas	66.4 nTPM
Spleen	50.8 nTPM
Testis	50.0 nTPM
Esophagus	42.5 nTPM
Adipose tissue	41.2 nTPM
Endometrium	40.3 nTPM
Bone marrow	40.0 nTPM
Prostate	35.5 nTPM
Kidney	32.5 nTPM
Ovary	30.9 nTPM
Breast	24.3 nTPM
PBMC	13.6 nTPM

Paneth cells	32.3 nTPM
Rectum	51.0 nTPM
Small intestine	26.3 nTPM
Colon	19.7 nTPM

Plasma cells	15.9 nTPM
Esophagus	24.6 nTPM
Stomach	23.4 nTPM
Adipose tissue	13.6 nTPM
Spleen	12.7 nTPM
Bone marrow	5.0 nTPM

Respiratory ciliated cells	66.6 nTPM
Bronchus	70.6 nTPM
Lung	62.7 nTPM

Smooth muscle cells	92.7 nTPM
Pancreas	243.8 nTPM
Adipose tissue	203.8 nTPM
Heart muscle	105.0 nTPM
Prostate	83.1 nTPM
Breast	71.2 nTPM
Skeletal muscle	68.4 nTPM
Skin	61.0 nTPM
Esophagus	58.9 nTPM
Endometrium	46.4 nTPM
Bronchus	42.2 nTPM
Ovary	35.9 nTPM

Basal keratinocytes	47.0 nTPM
Skin	47.0 nTPM

Basal prostatic cells	160.7 nTPM
Prostate	160.7 nTPM

Basal respiratory cells	126.0 nTPM
Bronchus	126.0 nTPM

Basal squamous epithelial cells	68.9 nTPM
Esophagus	68.9 nTPM

T-cells	13.6 nTPM
Skin	30.9 nTPM
Prostate	28.4 nTPM
Lung	27.4 nTPM
Endometrium	24.6 nTPM
Skeletal muscle	22.6 nTPM
Lymph node	10.3 nTPM
Breast	10.2 nTPM
Adipose tissue	9.3 nTPM
Liver	9.1 nTPM
Ovary	8.9 nTPM
PBMC	8.5 nTPM
Stomach	8.5 nTPM
Colon	7.6 nTPM
Spleen	7.3 nTPM
Esophagus	6.6 nTPM
Kidney	6.2 nTPM
Bone marrow	4.7 nTPM

Undifferentiated cells	67.3 nTPM
Colon	89.3 nTPM
Rectum	67.0 nTPM
Small intestine	45.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC