Single cell type - NACA2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NACA2
Gene descriptionⁱ Full gene name according to HGNC.	Nascent polypeptide associated complex subunit alpha 2
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Early spermatids, Spermatocytes, Late spermatids, Cardiomyocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (NB-4, SK-MEL-30)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Early spermatids, Spermatocytes, Late spermatids, Cardiomyocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NACA2 is part of cluster 10 Non-specific - Unknown function with confidenceⁱ 1
482 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PTTG2	Pituitary tumor-transforming 2	0.8456	10
CCT4	Chaperonin containing TCP1 subunit 4	0.7778	10
POTEM	POTE ankyrin domain family member M	0.7713	10
GMPS	Guanine monophosphate synthase	0.7518	10
GSTA5	Glutathione S-transferase alpha 5	0.7510	10
PAPOLB	Poly(A) polymerase beta	0.7505	10
TSN	Translin	0.7468	10
HMGA2	High mobility group AT-hook 2	0.7270	10
ZNF607	Zinc finger protein 607	0.7118	10
CSNK2A3	Casein kinase 2 alpha 3	0.7098	10
WDR12	WD repeat domain 12	0.7055	10
ANGPTL6	Angiopoietin like 6	0.6987	10
PSMD11	Proteasome 26S subunit, non-ATPase 11	0.6966	10
CCT5	Chaperonin containing TCP1 subunit 5	0.6943	10
ASNS	Asparagine synthetase (glutamine-hydrolyzing)	0.6876	10

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	0.7 nTPM
Breast	0.9 nTPM
Adipose tissue	0.5 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	0.0 nTPM
Lung	0.0 nTPM

Astrocytes	2.0 nTPM
Brain	2.0 nTPM

B-cells	11.2 nTPM
Liver	46.7 nTPM
PBMC	40.0 nTPM
Kidney	19.0 nTPM
Colon	3.2 nTPM
Adipose tissue	1.5 nTPM
Breast	1.3 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Dendritic cells	11.7 nTPM
PBMC	31.8 nTPM
Breast	2.5 nTPM
Adipose tissue	0.7 nTPM

Endothelial cells	17.6 nTPM
Heart muscle	68.7 nTPM
Skin	51.7 nTPM
Prostate	38.2 nTPM
Testis	28.2 nTPM
Liver	25.7 nTPM
Skeletal muscle	25.6 nTPM
Placenta	5.7 nTPM
Breast	1.1 nTPM
Endometrium	0.5 nTPM
Ovary	0.3 nTPM
Eye	0.0 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM
Esophagus	0.0 nTPM

Fibroblasts	18.5 nTPM
Heart muscle	95.2 nTPM
Skin	44.3 nTPM
Prostate	25.8 nTPM
Skeletal muscle	23.4 nTPM
Placenta	12.5 nTPM
Ovary	1.2 nTPM
Breast	1.0 nTPM
Adipose tissue	0.3 nTPM
Lung	0.0 nTPM
Esophagus	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	9.1 nTPM
Rectum	20.8 nTPM
Small intestine	6.4 nTPM
Colon	0.0 nTPM

Macrophages	8.2 nTPM
Skeletal muscle	42.6 nTPM
Kidney	31.0 nTPM
Testis	28.4 nTPM
Prostate	17.2 nTPM
Breast	1.6 nTPM
Ovary	0.9 nTPM
Adipose tissue	0.9 nTPM
Lung	0.0 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM
Endometrium	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	11.1 nTPM
Rectum	22.0 nTPM
Small intestine	11.3 nTPM
Colon	0.0 nTPM

Plasma cells	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Respiratory ciliated cells	2.9 nTPM
Bronchus	5.8 nTPM
Lung	0.0 nTPM

Smooth muscle cells	107.3 nTPM
Bronchus	953.9 nTPM
Heart muscle	98.1 nTPM
Skin	57.5 nTPM
Prostate	36.9 nTPM
Skeletal muscle	29.6 nTPM
Pancreas	1.4 nTPM
Breast	1.1 nTPM
Adipose tissue	0.7 nTPM
Endometrium	0.5 nTPM
Ovary	0.4 nTPM
Esophagus	0.0 nTPM

T-cells	16.3 nTPM
Skin	68.6 nTPM
Liver	60.1 nTPM
PBMC	56.6 nTPM
Kidney	33.6 nTPM
Skeletal muscle	30.9 nTPM
Prostate	19.0 nTPM
Colon	3.8 nTPM
Ovary	2.2 nTPM
Breast	1.3 nTPM
Adipose tissue	1.0 nTPM
Endometrium	0.6 nTPM
Lung	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Basal keratinocytes	69.3 nTPM
Skin	69.3 nTPM

Basal prostatic cells	60.6 nTPM
Prostate	60.6 nTPM

Basal respiratory cells	15.0 nTPM
Bronchus	15.0 nTPM

Cholangiocytes	35.8 nTPM
Liver	35.8 nTPM

Undifferentiated cells	11.8 nTPM
Rectum	19.7 nTPM
Small intestine	13.3 nTPM
Colon	2.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC