Single cell type - NCKAP1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NCKAP1
Gene descriptionⁱ Full gene name according to HGNC.	NCK associated protein 1
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Endometrium - Transcription (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NCKAP1 is part of cluster 44 Endometrium - Transcription with confidenceⁱ 1
491 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ARHGEF12	Rho guanine nucleotide exchange factor 12	0.6572	44
SLMAP	Sarcolemma associated protein	0.5588	54
CEACAM19	CEA cell adhesion molecule 19	0.5199	44
MPRIP	Myosin phosphatase Rho interacting protein	0.5174	48
DGKH	Diacylglycerol kinase eta	0.5166	44
PRKCI	Protein kinase C iota	0.4973	44
DAB2IP	DAB2 interacting protein	0.4953	44
UBE2H	Ubiquitin conjugating enzyme E2 H	0.4869	44
CRIM1	Cysteine rich transmembrane BMP regulator 1	0.4781	48
MAFK	MAF bZIP transcription factor K	0.4570	44
PTPN14	Protein tyrosine phosphatase non-receptor type 14	0.4512	14
KCNC4	Potassium voltage-gated channel subfamily C member 4	0.4478	9
PLCB4	Phospholipase C beta 4	0.4432	8
TAC4	Tachykinin precursor 4	0.4428	21
WWC1	WW and C2 domain containing 1	0.4402	44

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	75.3 nTPM
Adipose tissue	81.8 nTPM
Breast	68.8 nTPM

Alveolar cells type 1	48.1 nTPM
Lung	48.1 nTPM

Alveolar cells type 2	47.1 nTPM
Lung	47.1 nTPM

Astrocytes	129.9 nTPM
Brain	129.9 nTPM

B-cells	2.1 nTPM
Kidney	14.3 nTPM
Breast	5.3 nTPM
Liver	0.9 nTPM
Stomach	0.5 nTPM
Spleen	0.3 nTPM
Colon	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM

Dendritic cells	3.7 nTPM
Adipose tissue	6.3 nTPM
Breast	4.7 nTPM
PBMC	0.0 nTPM

Endothelial cells	82.8 nTPM
Prostate	132.2 nTPM
Skeletal muscle	108.6 nTPM
Ovary	103.7 nTPM
Testis	93.7 nTPM
Heart muscle	93.4 nTPM
Liver	87.2 nTPM
Esophagus	81.6 nTPM
Endometrium	80.8 nTPM
Skin	78.0 nTPM
Lung	74.8 nTPM
Breast	70.1 nTPM
Pancreas	54.0 nTPM
Placenta	52.8 nTPM
Eye	48.6 nTPM

Fibroblasts	62.3 nTPM
Prostate	83.3 nTPM
Skin	78.5 nTPM
Skeletal muscle	76.6 nTPM
Heart muscle	66.6 nTPM
Adipose tissue	65.7 nTPM
Esophagus	64.2 nTPM
Ovary	56.5 nTPM
Placenta	55.7 nTPM
Stomach	55.0 nTPM
Breast	44.4 nTPM
Lung	39.1 nTPM

Intestinal goblet cells	58.8 nTPM
Rectum	74.7 nTPM
Small intestine	60.9 nTPM
Colon	40.7 nTPM

Macrophages	4.6 nTPM
PBMC	13.6 nTPM
Kidney	11.4 nTPM
Endometrium	8.2 nTPM
Skeletal muscle	8.1 nTPM
Testis	5.8 nTPM
Pancreas	5.1 nTPM
Lung	5.0 nTPM
Prostate	4.6 nTPM
Esophagus	3.1 nTPM
Breast	2.2 nTPM
Ovary	1.7 nTPM
Adipose tissue	0.5 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	69.8 nTPM
Small intestine	101.4 nTPM
Rectum	71.8 nTPM
Colon	36.2 nTPM

Plasma cells	1.4 nTPM
Adipose tissue	3.9 nTPM
Stomach	2.9 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	40.2 nTPM
Bronchus	61.5 nTPM
Lung	19.0 nTPM

Smooth muscle cells	84.1 nTPM
Heart muscle	172.1 nTPM
Esophagus	114.7 nTPM
Prostate	112.6 nTPM
Skeletal muscle	91.5 nTPM
Adipose tissue	86.9 nTPM
Skin	81.9 nTPM
Endometrium	71.6 nTPM
Pancreas	64.1 nTPM
Breast	57.7 nTPM
Ovary	52.1 nTPM
Bronchus	19.8 nTPM

T-cells	5.5 nTPM
Endometrium	15.0 nTPM
Skin	12.4 nTPM
Prostate	9.5 nTPM
Lung	9.1 nTPM
Kidney	8.2 nTPM
Skeletal muscle	8.0 nTPM
Colon	5.7 nTPM
Breast	4.8 nTPM
Ovary	4.5 nTPM
Bone marrow	3.1 nTPM
Liver	3.0 nTPM
Spleen	2.9 nTPM
Adipose tissue	2.7 nTPM
Stomach	1.8 nTPM
Lymph node	1.7 nTPM
PBMC	1.4 nTPM
Esophagus	0.0 nTPM

Basal prostatic cells	64.7 nTPM
Prostate	64.7 nTPM

Basal respiratory cells	50.1 nTPM
Bronchus	50.1 nTPM

Basal squamous epithelial cells	38.1 nTPM
Esophagus	38.1 nTPM

Collecting duct cells	80.9 nTPM
Kidney	80.9 nTPM

Undifferentiated cells	55.5 nTPM
Rectum	61.1 nTPM
Small intestine	58.0 nTPM
Colon	47.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC