Single cell type - ACTN1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ACTN1
Gene descriptionⁱ Full gene name according to HGNC.	Actinin alpha 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes FDA approved drug targets Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	15

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Pancreatic cells - Digestion (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Extravillous trophoblasts)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (neutrophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Extravillous trophoblasts)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ACTN1 is part of cluster 1 Pancreatic cells - Digestion with confidenceⁱ 1
148 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PDLIM7	PDZ and LIM domain 7	0.6612	1
CSRP1	Cysteine and glycine rich protein 1	0.5947	48
SPHK1	Sphingosine kinase 1	0.5842	1
CALD1	Caldesmon 1	0.5833	48
NUTF2	Nuclear transport factor 2	0.5792	10
KRT75	Keratin 75	0.5770	1
CCL2	C-C motif chemokine ligand 2	0.5685	48
NPPB	Natriuretic peptide B	0.5634	1
MYLK	Myosin light chain kinase	0.5596	48
RSU1	Ras suppressor protein 1	0.5546	51
COL27A1	Collagen type XXVII alpha 1 chain	0.5457	48
BRS3	Bombesin receptor subtype 3	0.5419	1
TUBB	Tubulin beta class I	0.5400	10
MSANTD3	Myb/SANT DNA binding domain containing 3	0.5386	51
TAGLN	Transgelin	0.5375	48

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	44.7 nTPM
Adipose tissue	56.8 nTPM
Breast	32.6 nTPM

Alveolar cells type 1	38.5 nTPM
Lung	38.5 nTPM

Alveolar cells type 2	25.1 nTPM
Lung	25.1 nTPM

Astrocytes	19.6 nTPM
Brain	19.6 nTPM

B-cells	4.0 nTPM
Colon	17.6 nTPM
PBMC	4.3 nTPM
Bone marrow	4.1 nTPM
Lymph node	3.8 nTPM
Liver	3.7 nTPM
Spleen	3.1 nTPM
Breast	2.6 nTPM
Adipose tissue	1.0 nTPM
Kidney	0.0 nTPM
Stomach	0.0 nTPM

Dendritic cells	3.0 nTPM
Breast	5.4 nTPM
Adipose tissue	3.4 nTPM
PBMC	0.0 nTPM

Endothelial cells	135.5 nTPM
Skin	257.4 nTPM
Esophagus	244.0 nTPM
Testis	208.9 nTPM
Liver	206.3 nTPM
Prostate	154.1 nTPM
Endometrium	107.2 nTPM
Skeletal muscle	104.3 nTPM
Pancreas	104.2 nTPM
Placenta	101.8 nTPM
Lung	99.8 nTPM
Heart muscle	83.2 nTPM
Eye	81.7 nTPM
Breast	79.1 nTPM
Ovary	65.0 nTPM

Fibroblasts	88.9 nTPM
Placenta	184.4 nTPM
Ovary	148.6 nTPM
Prostate	145.2 nTPM
Stomach	115.9 nTPM
Skin	95.2 nTPM
Heart muscle	84.9 nTPM
Lung	69.2 nTPM
Breast	61.9 nTPM
Adipose tissue	25.2 nTPM
Esophagus	24.0 nTPM
Skeletal muscle	22.9 nTPM

Intestinal goblet cells	57.3 nTPM
Colon	75.8 nTPM
Rectum	61.4 nTPM
Small intestine	34.7 nTPM

Macrophages	96.4 nTPM
PBMC	518.3 nTPM
Pancreas	150.6 nTPM
Lung	116.6 nTPM
Endometrium	78.6 nTPM
Spleen	70.5 nTPM
Prostate	68.8 nTPM
Adipose tissue	66.0 nTPM
Skeletal muscle	58.8 nTPM
Kidney	56.7 nTPM
Esophagus	56.6 nTPM
Stomach	52.7 nTPM
Testis	48.3 nTPM
Bone marrow	47.5 nTPM
Breast	32.2 nTPM
Ovary	24.1 nTPM

Paneth cells	28.0 nTPM
Rectum	47.1 nTPM
Small intestine	33.8 nTPM
Colon	3.3 nTPM

Plasma cells	0.6 nTPM
Adipose tissue	2.7 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Respiratory ciliated cells	47.8 nTPM
Bronchus	56.2 nTPM
Lung	39.4 nTPM

Smooth muscle cells	270.6 nTPM
Pancreas	898.5 nTPM
Bronchus	297.5 nTPM
Heart muscle	253.6 nTPM
Esophagus	246.6 nTPM
Prostate	221.3 nTPM
Skin	203.0 nTPM
Skeletal muscle	193.3 nTPM
Endometrium	183.3 nTPM
Breast	177.0 nTPM
Ovary	173.3 nTPM
Adipose tissue	129.4 nTPM

T-cells	22.7 nTPM
PBMC	82.8 nTPM
Lymph node	78.0 nTPM
Prostate	33.1 nTPM
Spleen	30.0 nTPM
Lung	22.8 nTPM
Endometrium	22.0 nTPM
Skin	20.2 nTPM
Kidney	17.1 nTPM
Bone marrow	17.1 nTPM
Esophagus	13.1 nTPM
Liver	11.2 nTPM
Skeletal muscle	11.2 nTPM
Colon	7.6 nTPM
Breast	5.8 nTPM
Stomach	5.5 nTPM
Ovary	4.5 nTPM
Adipose tissue	4.4 nTPM

Basal keratinocytes	119.3 nTPM
Skin	119.3 nTPM

Basal prostatic cells	154.9 nTPM
Prostate	154.9 nTPM

Basal respiratory cells	137.4 nTPM
Bronchus	137.4 nTPM

Basal squamous epithelial cells	82.0 nTPM
Esophagus	82.0 nTPM

Undifferentiated cells	47.0 nTPM
Rectum	52.3 nTPM
Colon	52.1 nTPM
Small intestine	36.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC