Single cell type - ENO1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ENO1
Gene descriptionⁱ Full gene name according to HGNC.	Enolase 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	8

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Pancreatic endocrine cells - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Muller glia cells, Ductal cells, Extravillous trophoblasts)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Muller glia cells, Ductal cells, Extravillous trophoblasts)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ENO1 is part of cluster 16 Pancreatic endocrine cells - Mixed function with confidenceⁱ 1
171 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PKM	Pyruvate kinase M1/2	0.7100	16
TPI1	Triosephosphate isomerase 1	0.7069	16
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	0.6944	16
PGAM1	Phosphoglycerate mutase 1	0.5905	16
PPA1	Inorganic pyrophosphatase 1	0.5902	5
LDHA	Lactate dehydrogenase A	0.5778	16
PGK1	Phosphoglycerate kinase 1	0.5748	26
KRT7	Keratin 7	0.5540	16
CFL1	Cofilin 1	0.5500	16
PGM2	Phosphoglucomutase 2	0.5396	5
JPT1	Jupiter microtubule associated homolog 1	0.5326	7
ACTG1	Actin gamma 1	0.5275	22
S100A11	S100 calcium binding protein A11	0.5245	16
SNAPIN	SNAP associated protein	0.5219	26
ITPA	Inosine triphosphatase	0.5201	39

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	140.0 nTPM
Breast	147.8 nTPM
Adipose tissue	132.3 nTPM

Alveolar cells type 1	278.9 nTPM
Lung	278.9 nTPM

Alveolar cells type 2	499.1 nTPM
Lung	499.1 nTPM

Astrocytes	99.6 nTPM
Brain	99.6 nTPM

B-cells	222.3 nTPM
Liver	543.1 nTPM
Kidney	299.9 nTPM
PBMC	245.6 nTPM
Colon	234.8 nTPM
Spleen	219.5 nTPM
Lymph node	151.4 nTPM
Stomach	149.4 nTPM
Breast	132.4 nTPM
Bone marrow	127.6 nTPM
Adipose tissue	119.8 nTPM

Basal squamous epithelial cells	348.8 nTPM
Esophagus	348.8 nTPM

Dendritic cells	194.0 nTPM
Adipose tissue	234.4 nTPM
Breast	200.1 nTPM
PBMC	147.4 nTPM

Endothelial cells	314.3 nTPM
Pancreas	1065.5 nTPM
Prostate	647.3 nTPM
Placenta	455.8 nTPM
Ovary	395.7 nTPM
Skin	265.2 nTPM
Eye	254.1 nTPM
Lung	205.9 nTPM
Testis	205.0 nTPM
Endometrium	194.0 nTPM
Skeletal muscle	161.5 nTPM
Esophagus	155.9 nTPM
Liver	149.5 nTPM
Breast	124.1 nTPM
Heart muscle	120.6 nTPM

Enteroendocrine cells	189.0 nTPM
Rectum	203.9 nTPM
Colon	174.1 nTPM

Erythroid cells	145.4 nTPM
Bone marrow	248.0 nTPM
Liver	42.9 nTPM

Fibroblasts	303.5 nTPM
Lung	573.0 nTPM
Ovary	553.6 nTPM
Placenta	508.7 nTPM
Prostate	401.4 nTPM
Skin	259.4 nTPM
Stomach	222.7 nTPM
Adipose tissue	193.4 nTPM
Skeletal muscle	186.5 nTPM
Heart muscle	179.1 nTPM
Breast	147.7 nTPM
Esophagus	113.2 nTPM

Intestinal goblet cells	297.3 nTPM
Colon	411.6 nTPM
Small intestine	284.1 nTPM
Rectum	196.1 nTPM

Macrophages	454.2 nTPM
Pancreas	1408.9 nTPM
Kidney	664.8 nTPM
Lung	542.3 nTPM
Prostate	488.3 nTPM
Testis	461.8 nTPM
Adipose tissue	434.3 nTPM
Stomach	416.1 nTPM
Endometrium	370.4 nTPM
Bone marrow	354.9 nTPM
Spleen	350.4 nTPM
Skeletal muscle	310.5 nTPM
Esophagus	281.7 nTPM
Breast	260.2 nTPM
Ovary	236.4 nTPM
PBMC	231.9 nTPM

Paneth cells	366.9 nTPM
Colon	467.7 nTPM
Small intestine	360.5 nTPM
Rectum	272.4 nTPM

Plasma cells	211.0 nTPM
Spleen	369.5 nTPM
Bone marrow	347.7 nTPM
Stomach	123.5 nTPM
Esophagus	110.8 nTPM
Adipose tissue	103.2 nTPM

Cholangiocytes	283.1 nTPM
Liver	283.1 nTPM

Collecting duct cells	606.9 nTPM
Kidney	606.9 nTPM

Cone photoreceptor cells	70.9 nTPM
Eye	70.9 nTPM

Cytotrophoblasts	1045.4 nTPM
Placenta	1045.4 nTPM

Respiratory ciliated cells	245.7 nTPM
Bronchus	271.3 nTPM
Lung	220.1 nTPM

Smooth muscle cells	402.5 nTPM
Pancreas	2260.3 nTPM
Ovary	478.2 nTPM
Bronchus	371.7 nTPM
Prostate	296.3 nTPM
Skin	232.6 nTPM
Skeletal muscle	159.4 nTPM
Endometrium	148.7 nTPM
Breast	142.4 nTPM
Heart muscle	129.5 nTPM
Adipose tissue	107.1 nTPM
Esophagus	100.8 nTPM

T-cells	285.6 nTPM
Skin	457.7 nTPM
Kidney	431.9 nTPM
Lung	350.8 nTPM
Skeletal muscle	329.6 nTPM
Adipose tissue	308.4 nTPM
PBMC	308.0 nTPM
Colon	307.9 nTPM
Liver	304.4 nTPM
Stomach	249.3 nTPM
Prostate	241.4 nTPM
Endometrium	241.3 nTPM
Esophagus	229.3 nTPM
Spleen	229.0 nTPM
Ovary	226.9 nTPM
Bone marrow	222.3 nTPM
Breast	209.3 nTPM
Lymph node	208.4 nTPM

Undifferentiated cells	528.4 nTPM
Colon	787.7 nTPM
Small intestine	460.8 nTPM
Rectum	336.6 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC