Single cell type - RHBDF2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RHBDF2
Gene descriptionⁱ Full gene name according to HGNC.	Rhomboid 5 homolog 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	14

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	B-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (NK-cells, B-cells, Kupffer cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (RPMI-8226)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (NK-cells, B-cells, Kupffer cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RHBDF2 is part of cluster 57 B-cells - Immune response with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CD72	CD72 molecule	0.7120	57
ADAM28	ADAM metallopeptidase domain 28	0.6929	57
DRAM2	DNA damage regulated autophagy modulator 2	0.6239	57
PTPN6	Protein tyrosine phosphatase non-receptor type 6	0.6125	57
INPP5D	Inositol polyphosphate-5-phosphatase D	0.5972	63
FCER2	Fc fragment of IgE receptor II	0.5885	57
TLR6	Toll like receptor 6	0.5869	57
FGD2	FYVE, RhoGEF and PH domain containing 2	0.5827	57
LYN	LYN proto-oncogene, Src family tyrosine kinase	0.5776	57
RCSD1	RCSD domain containing 1	0.5753	57
CD37	CD37 molecule	0.5716	57
CD180	CD180 molecule	0.5691	57
FCRL3	Fc receptor like 3	0.5658	57
GSAP	Gamma-secretase activating protein	0.5650	63
SIPA1	Signal-induced proliferation-associated 1	0.5591	63

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	22.3 nTPM
Adipose tissue	33.5 nTPM
Breast	11.1 nTPM

Alveolar cells type 1	6.4 nTPM
Lung	6.4 nTPM

Alveolar cells type 2	6.9 nTPM
Lung	6.9 nTPM

Astrocytes	2.3 nTPM
Brain	2.3 nTPM

Basal keratinocytes	17.6 nTPM
Skin	17.6 nTPM

B-cells	58.4 nTPM
Bone marrow	92.3 nTPM
Lymph node	81.5 nTPM
Stomach	78.6 nTPM
PBMC	67.1 nTPM
Colon	65.5 nTPM
Adipose tissue	57.2 nTPM
Spleen	46.8 nTPM
Breast	38.8 nTPM
Kidney	28.5 nTPM
Liver	27.2 nTPM

Dendritic cells	47.1 nTPM
Adipose tissue	62.6 nTPM
PBMC	39.8 nTPM
Breast	39.0 nTPM

Endothelial cells	15.3 nTPM
Eye	54.2 nTPM
Prostate	30.9 nTPM
Skeletal muscle	30.7 nTPM
Ovary	18.8 nTPM
Esophagus	13.9 nTPM
Testis	13.3 nTPM
Skin	13.3 nTPM
Heart muscle	12.3 nTPM
Breast	7.8 nTPM
Placenta	4.0 nTPM
Pancreas	3.9 nTPM
Endometrium	3.9 nTPM
Liver	3.7 nTPM
Lung	3.1 nTPM

Fibroblasts	4.7 nTPM
Lung	12.3 nTPM
Placenta	9.6 nTPM
Prostate	6.3 nTPM
Skin	6.0 nTPM
Stomach	6.0 nTPM
Heart muscle	4.9 nTPM
Ovary	3.1 nTPM
Skeletal muscle	1.7 nTPM
Esophagus	1.1 nTPM
Breast	1.0 nTPM
Adipose tissue	0.3 nTPM

Intestinal goblet cells	4.1 nTPM
Small intestine	5.0 nTPM
Rectum	4.4 nTPM
Colon	2.9 nTPM

Macrophages	48.8 nTPM
Adipose tissue	99.8 nTPM
Prostate	97.4 nTPM
Testis	69.2 nTPM
Ovary	68.4 nTPM
Skeletal muscle	56.3 nTPM
Stomach	49.8 nTPM
Esophagus	48.7 nTPM
Spleen	47.7 nTPM
Bone marrow	45.0 nTPM
Pancreas	35.7 nTPM
Kidney	31.8 nTPM
Endometrium	30.8 nTPM
Breast	28.9 nTPM
Lung	22.6 nTPM
PBMC	0.0 nTPM

Paneth cells	4.8 nTPM
Rectum	7.3 nTPM
Small intestine	3.7 nTPM
Colon	3.3 nTPM

Plasma cells	36.5 nTPM
Adipose tissue	60.0 nTPM
Stomach	39.7 nTPM
Esophagus	36.9 nTPM
Bone marrow	26.4 nTPM
Spleen	19.5 nTPM

Respiratory ciliated cells	16.2 nTPM
Bronchus	30.9 nTPM
Lung	1.5 nTPM

Smooth muscle cells	3.2 nTPM
Bronchus	9.5 nTPM
Pancreas	5.5 nTPM
Skin	3.9 nTPM
Prostate	3.7 nTPM
Ovary	3.4 nTPM
Heart muscle	3.0 nTPM
Skeletal muscle	2.7 nTPM
Breast	1.4 nTPM
Endometrium	0.9 nTPM
Adipose tissue	0.9 nTPM
Esophagus	0.0 nTPM

Cholangiocytes	18.6 nTPM
Liver	18.6 nTPM

Cone photoreceptor cells	2.1 nTPM
Eye	2.1 nTPM

Early spermatids	0.5 nTPM
Testis	0.5 nTPM

Endometrial stromal cells	0.8 nTPM
Endometrium	0.8 nTPM

T-cells	22.3 nTPM
Colon	39.0 nTPM
Skeletal muscle	37.8 nTPM
Kidney	32.9 nTPM
Ovary	31.1 nTPM
Liver	28.4 nTPM
Adipose tissue	27.2 nTPM
PBMC	24.4 nTPM
Spleen	23.7 nTPM
Bone marrow	23.3 nTPM
Esophagus	19.7 nTPM
Endometrium	17.6 nTPM
Stomach	16.3 nTPM
Breast	14.6 nTPM
Lymph node	14.4 nTPM
Skin	14.3 nTPM
Lung	9.1 nTPM
Prostate	4.7 nTPM

Undifferentiated cells	7.8 nTPM
Small intestine	9.8 nTPM
Colon	7.2 nTPM
Rectum	6.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC