Single cell type - SPRYD4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SPRYD4
Gene descriptionⁱ Full gene name according to HGNC.	SPRY domain containing 4
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Proximal tubular cells - Tubular reabsorption (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Hepatocytes, Proximal tubular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Karpas-707)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Hepatocytes, Proximal tubular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

SPRYD4 is part of cluster 67 Proximal tubular cells - Tubular reabsorption with confidenceⁱ 1
499 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ACSM2A	Acyl-CoA synthetase medium chain family member 2A	0.6510	67
UPB1	Beta-ureidopropionase 1	0.6312	67
HPN	Hepsin	0.6254	67
ALDH4A1	Aldehyde dehydrogenase 4 family member A1	0.6185	67
MMAB	Metabolism of cobalamin associated B	0.6116	15
ALDH8A1	Aldehyde dehydrogenase 8 family member A1	0.5989	67
HMGCL	3-hydroxy-3-methylglutaryl-CoA lyase	0.5820	67
DCXR	Dicarbonyl and L-xylulose reductase	0.5761	67
SLC22A7	Solute carrier family 22 member 7	0.5715	67
MRPL44	Mitochondrial ribosomal protein L44	0.5710	47
HSD17B10	Hydroxysteroid 17-beta dehydrogenase 10	0.5699	67
SDHC	Succinate dehydrogenase complex subunit C	0.5647	67
PIPOX	Pipecolic acid and sarcosine oxidase	0.5619	67
MRPL19	Mitochondrial ribosomal protein L19	0.5598	39
FTCD	Formimidoyltransferase cyclodeaminase	0.5596	67

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	4.4 nTPM
Breast	4.9 nTPM
Adipose tissue	3.9 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	7.4 nTPM
Lung	7.4 nTPM

Astrocytes	2.0 nTPM
Brain	2.0 nTPM

B-cells	7.9 nTPM
Liver	21.1 nTPM
Kidney	19.0 nTPM
Bone marrow	10.6 nTPM
Spleen	8.3 nTPM
Colon	6.4 nTPM
PBMC	4.3 nTPM
Stomach	4.1 nTPM
Adipose tissue	2.5 nTPM
Breast	1.3 nTPM
Lymph node	1.1 nTPM

Dendritic cells	5.8 nTPM
PBMC	8.0 nTPM
Breast	5.8 nTPM
Adipose tissue	3.6 nTPM

Endothelial cells	8.9 nTPM
Pancreas	30.9 nTPM
Testis	10.6 nTPM
Liver	10.1 nTPM
Placenta	9.7 nTPM
Ovary	9.4 nTPM
Heart muscle	7.6 nTPM
Esophagus	7.3 nTPM
Eye	6.5 nTPM
Lung	6.2 nTPM
Breast	5.8 nTPM
Endometrium	5.4 nTPM
Prostate	5.3 nTPM
Skin	5.1 nTPM
Skeletal muscle	4.3 nTPM

Fibroblasts	8.1 nTPM
Ovary	18.1 nTPM
Placenta	13.1 nTPM
Heart muscle	10.1 nTPM
Breast	7.9 nTPM
Esophagus	6.8 nTPM
Lung	6.7 nTPM
Skeletal muscle	6.5 nTPM
Adipose tissue	5.9 nTPM
Prostate	5.5 nTPM
Stomach	4.4 nTPM
Skin	3.9 nTPM

Intestinal goblet cells	14.6 nTPM
Small intestine	18.0 nTPM
Rectum	15.2 nTPM
Colon	10.7 nTPM

Macrophages	6.4 nTPM
Kidney	17.5 nTPM
Pancreas	12.8 nTPM
Ovary	11.3 nTPM
Testis	10.5 nTPM
Endometrium	6.5 nTPM
Esophagus	6.3 nTPM
Lung	5.8 nTPM
Breast	4.6 nTPM
Skeletal muscle	4.4 nTPM
Spleen	4.2 nTPM
Bone marrow	3.7 nTPM
Prostate	3.4 nTPM
Stomach	2.9 nTPM
Adipose tissue	2.3 nTPM
PBMC	0.0 nTPM

Paneth cells	10.1 nTPM
Rectum	16.2 nTPM
Small intestine	7.5 nTPM
Colon	6.5 nTPM

Plasma cells	6.3 nTPM
Stomach	13.7 nTPM
Bone marrow	6.6 nTPM
Spleen	5.9 nTPM
Adipose tissue	5.2 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	5.9 nTPM
Bronchus	8.9 nTPM
Lung	2.9 nTPM

Smooth muscle cells	8.6 nTPM
Heart muscle	23.7 nTPM
Pancreas	15.7 nTPM
Bronchus	9.2 nTPM
Skeletal muscle	8.0 nTPM
Ovary	6.6 nTPM
Breast	6.3 nTPM
Esophagus	6.2 nTPM
Endometrium	5.0 nTPM
Skin	4.9 nTPM
Prostate	4.7 nTPM
Adipose tissue	4.3 nTPM

T-cells	4.4 nTPM
Kidney	10.2 nTPM
Liver	9.6 nTPM
PBMC	6.5 nTPM
Stomach	6.2 nTPM
Bone marrow	5.8 nTPM
Colon	5.7 nTPM
Skeletal muscle	4.9 nTPM
Lung	4.5 nTPM
Endometrium	4.2 nTPM
Skin	4.1 nTPM
Adipose tissue	4.0 nTPM
Spleen	2.7 nTPM
Breast	2.5 nTPM
Ovary	2.2 nTPM
Lymph node	1.1 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	4.8 nTPM
Skin	4.8 nTPM

Basal respiratory cells	24.8 nTPM
Bronchus	24.8 nTPM

Cholangiocytes	18.6 nTPM
Liver	18.6 nTPM

Collecting duct cells	22.0 nTPM
Kidney	22.0 nTPM

Undifferentiated cells	16.4 nTPM
Small intestine	18.8 nTPM
Rectum	18.4 nTPM
Colon	12.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC