Single cell type - ERN1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ERN1
Gene descriptionⁱ Full gene name according to HGNC.	Endoplasmic reticulum to nucleus signaling 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins Plasma proteins Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Plasma cells - Humoral immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (basophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Karpas-707, RPMI-8226, U-266/70)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ERN1 is part of cluster 55 Plasma cells - Humoral immune response with confidenceⁱ 1
379 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
BCAS2	BCAS2 pre-mRNA processing factor	0.6951	7
DUSP5	Dual specificity phosphatase 5	0.6694	22
CLK1	CDC like kinase 1	0.6525	22
RGS1	Regulator of G protein signaling 1	0.6230	41
DNAJB9	DnaJ heat shock protein family (Hsp40) member B9	0.6172	55
IGKV1D-13	Immunoglobulin kappa variable 1D-13	0.6046	55
IGLV1-50	Immunoglobulin lambda variable 1-50 (non-functional)	0.6044	55
TRAM1	Translocation associated membrane protein 1	0.6031	55
DDIT4	DNA damage inducible transcript 4	0.5926	22
IGLV1-51	Immunoglobulin lambda variable 1-51	0.5878	55
GPR15	G protein-coupled receptor 15	0.5846	55
IGKV2-28	Immunoglobulin kappa variable 2-28	0.5833	55
IGHA2	Immunoglobulin heavy constant alpha 2 (A2m marker)	0.5819	55
MCL1	MCL1 apoptosis regulator, BCL2 family member	0.5805	41
PPP1R15A	Protein phosphatase 1 regulatory subunit 15A	0.5800	7

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	31.9 nTPM
Breast	39.9 nTPM
Adipose tissue	23.9 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	36.1 nTPM
Lung	36.1 nTPM

Astrocytes	7.2 nTPM
Brain	7.2 nTPM

B-cells	27.8 nTPM
Breast	140.5 nTPM
Liver	48.8 nTPM
Lymph node	23.5 nTPM
Adipose tissue	14.6 nTPM
Spleen	11.8 nTPM
Stomach	11.3 nTPM
Colon	9.6 nTPM
Kidney	9.5 nTPM
Bone marrow	5.7 nTPM
PBMC	2.8 nTPM

Dendritic cells	106.4 nTPM
Adipose tissue	160.5 nTPM
PBMC	91.6 nTPM
Breast	67.0 nTPM

Endothelial cells	18.3 nTPM
Prostate	59.1 nTPM
Skin	28.4 nTPM
Skeletal muscle	27.9 nTPM
Breast	27.5 nTPM
Esophagus	27.3 nTPM
Endometrium	20.6 nTPM
Eye	12.9 nTPM
Pancreas	11.6 nTPM
Heart muscle	11.5 nTPM
Ovary	10.7 nTPM
Liver	8.2 nTPM
Testis	3.5 nTPM
Placenta	3.4 nTPM
Lung	3.1 nTPM

Enteroendocrine cells	79.7 nTPM
Colon	127.9 nTPM
Rectum	31.6 nTPM

Fibroblasts	42.2 nTPM
Prostate	252.8 nTPM
Esophagus	48.5 nTPM
Stomach	41.6 nTPM
Skin	36.8 nTPM
Breast	24.5 nTPM
Adipose tissue	18.8 nTPM
Skeletal muscle	16.7 nTPM
Lung	6.7 nTPM
Ovary	6.6 nTPM
Heart muscle	6.4 nTPM
Placenta	5.2 nTPM

Intestinal goblet cells	8.2 nTPM
Colon	10.7 nTPM
Rectum	7.7 nTPM
Small intestine	6.1 nTPM

Macrophages	60.4 nTPM
Prostate	259.0 nTPM
Breast	143.1 nTPM
Adipose tissue	131.7 nTPM
Endometrium	89.2 nTPM
Esophagus	66.1 nTPM
Spleen	61.1 nTPM
Stomach	49.8 nTPM
Skeletal muscle	39.4 nTPM
Kidney	24.9 nTPM
Ovary	14.8 nTPM
Bone marrow	10.0 nTPM
Lung	8.2 nTPM
Pancreas	7.7 nTPM
Testis	1.7 nTPM
PBMC	0.0 nTPM

Paneth cells	7.3 nTPM
Colon	9.9 nTPM
Rectum	8.3 nTPM
Small intestine	3.7 nTPM

Plasma cells	325.8 nTPM
Stomach	494.7 nTPM
Esophagus	492.7 nTPM
Bone marrow	255.4 nTPM
Spleen	222.3 nTPM
Adipose tissue	163.9 nTPM

Respiratory ciliated cells	6.1 nTPM
Lung	7.3 nTPM
Bronchus	5.0 nTPM

Smooth muscle cells	25.7 nTPM
Prostate	80.7 nTPM
Endometrium	50.0 nTPM
Esophagus	37.2 nTPM
Breast	26.7 nTPM
Adipose tissue	21.8 nTPM
Skin	16.7 nTPM
Skeletal muscle	16.6 nTPM
Ovary	11.4 nTPM
Pancreas	10.9 nTPM
Bronchus	6.6 nTPM
Heart muscle	3.9 nTPM

Basal keratinocytes	22.0 nTPM
Skin	22.0 nTPM

Basal prostatic cells	54.3 nTPM
Prostate	54.3 nTPM

Basal squamous epithelial cells	15.4 nTPM
Esophagus	15.4 nTPM

Cone photoreceptor cells	6.2 nTPM
Eye	6.2 nTPM

T-cells	88.2 nTPM
Prostate	307.7 nTPM
Adipose tissue	175.0 nTPM
Ovary	133.5 nTPM
Stomach	114.8 nTPM
Bone marrow	88.4 nTPM
Breast	80.2 nTPM
Spleen	78.0 nTPM
Liver	77.1 nTPM
Esophagus	72.0 nTPM
Endometrium	67.2 nTPM
Skeletal muscle	59.7 nTPM
Lymph node	55.8 nTPM
Colon	53.7 nTPM
Kidney	45.8 nTPM
Skin	40.9 nTPM
PBMC	31.4 nTPM
Lung	18.2 nTPM

Undifferentiated cells	9.6 nTPM
Colon	13.2 nTPM
Small intestine	9.1 nTPM
Rectum	6.6 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC