The localization of Calaxin (gene: CLXN) in ciliated cells is highlighted in fallopian tube by multiplex immunohistochemistry (mIHC/IF).

In the recently launched version 24 of The Human Protein Atlas, additional cell types and tissues have been added to the mIHC/IF-based exploration of protein expression, including ciliated cells in the fallopian tube. A ciliated cells panel made up of five markers for different subcellular structures in ciliated cells was created to localize the subcellular distribution of cilia-associated proteins. CLXN - a component of the protein complexes that facilitate ciliary motility - shows localization (green) in the cilia axoneme (white). Other visualized cell structures include cilia transition zone (yellow), cilia basal body (red), cytoplasm (cyan) and nucleus (magenta).

The spatial expression data is further supported by the single-cell RNA sequencing data for CLXN available in the Single Cell resource showing group enriched expression in ciliated cells and spermatids. Spermatids are equipped with a cilia-like tail structure, the flagellum, that share a lot of the protein building blocks with motile cilia.

For more details about the mIHC/IF technique visit our method page.