Single cell type - PLEKHO1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PLEKHO1
Gene descriptionⁱ Full gene name according to HGNC.	Pleckstrin homology domain containing O1
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids, Langerhans cells, monocytes, Macrophages)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids, Langerhans cells, monocytes, Macrophages)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PLEKHO1 is part of cluster 10 Non-specific - Unknown function with confidenceⁱ 1
482 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CALM3	Calmodulin 3	0.6564	34
TLN1	Talin 1	0.5970	51
TAF10	TATA-box binding protein associated factor 10	0.5730	10
PF4V1	Platelet factor 4 variant 1	0.5687	10
RAP1A	RAP1A, member of RAS oncogene family	0.5439	10
SLC39A3	Solute carrier family 39 member 3	0.5404	66
CSNK2A2	Casein kinase 2 alpha 2	0.5275	34
TIFAB	TIFA inhibitor	0.5259	41
LSM12	LSM12 homolog	0.5251	34
RAP1B	RAP1B, member of RAS oncogene family	0.5240	10
SMIM35	Small integral membrane protein 35	0.5159	10
ETV3L	ETS variant transcription factor 3 like	0.4960	10
SNAP23	Synaptosome associated protein 23	0.4949	51
ATXN7L3	Ataxin 7 like 3	0.4858	10
TM6SF1	Transmembrane 6 superfamily member 1	0.4819	10

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	23.5 nTPM
Breast	24.4 nTPM
Adipose tissue	22.6 nTPM

Alveolar cells type 1	35.3 nTPM
Lung	35.3 nTPM

Alveolar cells type 2	8.9 nTPM
Lung	8.9 nTPM

Astrocytes	32.3 nTPM
Brain	32.3 nTPM

B-cells	65.1 nTPM
Adipose tissue	117.8 nTPM
Breast	103.0 nTPM
Colon	95.8 nTPM
PBMC	95.7 nTPM
Spleen	48.4 nTPM
Liver	47.1 nTPM
Lymph node	40.2 nTPM
Bone marrow	39.2 nTPM
Kidney	38.1 nTPM
Stomach	25.9 nTPM

Dendritic cells	97.5 nTPM
Adipose tissue	131.1 nTPM
PBMC	99.6 nTPM
Breast	62.0 nTPM

Endothelial cells	58.9 nTPM
Prostate	120.3 nTPM
Testis	110.2 nTPM
Endometrium	108.5 nTPM
Liver	102.8 nTPM
Ovary	94.6 nTPM
Pancreas	54.0 nTPM
Eye	47.7 nTPM
Placenta	42.6 nTPM
Skin	38.8 nTPM
Esophagus	32.0 nTPM
Breast	28.8 nTPM
Skeletal muscle	15.8 nTPM
Lung	15.6 nTPM
Heart muscle	12.6 nTPM

Fibroblasts	29.4 nTPM
Prostate	91.2 nTPM
Ovary	44.7 nTPM
Skin	42.9 nTPM
Breast	30.6 nTPM
Placenta	24.9 nTPM
Heart muscle	21.7 nTPM
Adipose tissue	21.3 nTPM
Stomach	14.9 nTPM
Skeletal muscle	11.1 nTPM
Esophagus	10.6 nTPM
Lung	10.1 nTPM

Intestinal goblet cells	0.9 nTPM
Colon	2.2 nTPM
Rectum	0.6 nTPM
Small intestine	0.0 nTPM

Macrophages	147.9 nTPM
PBMC	504.7 nTPM
Endometrium	203.3 nTPM
Breast	202.7 nTPM
Ovary	158.0 nTPM
Kidney	152.8 nTPM
Skeletal muscle	137.9 nTPM
Adipose tissue	136.9 nTPM
Prostate	127.2 nTPM
Bone marrow	121.2 nTPM
Spleen	105.7 nTPM
Pancreas	104.7 nTPM
Testis	101.6 nTPM
Esophagus	99.1 nTPM
Stomach	38.1 nTPM
Lung	25.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	23.7 nTPM
Adipose tissue	61.3 nTPM
Esophagus	24.6 nTPM
Spleen	19.5 nTPM
Stomach	8.3 nTPM
Bone marrow	5.0 nTPM

Respiratory ciliated cells	1.3 nTPM
Bronchus	2.6 nTPM
Lung	0.0 nTPM

Smooth muscle cells	105.0 nTPM
Skin	276.4 nTPM
Prostate	170.7 nTPM
Ovary	153.4 nTPM
Pancreas	96.7 nTPM
Bronchus	88.3 nTPM
Breast	75.9 nTPM
Adipose tissue	72.2 nTPM
Heart muscle	63.4 nTPM
Skeletal muscle	57.9 nTPM
Esophagus	51.2 nTPM
Endometrium	49.4 nTPM

T-cells	45.6 nTPM
Adipose tissue	154.6 nTPM
Breast	81.8 nTPM
Endometrium	75.2 nTPM
Skeletal muscle	71.8 nTPM
Skin	57.5 nTPM
Esophagus	52.4 nTPM
Ovary	42.2 nTPM
Colon	38.5 nTPM
Liver	31.4 nTPM
PBMC	30.2 nTPM
Prostate	28.4 nTPM
Kidney	24.7 nTPM
Lung	21.3 nTPM
Stomach	18.9 nTPM
Spleen	18.8 nTPM
Lymph node	15.2 nTPM
Bone marrow	12.7 nTPM

Basal keratinocytes	21.5 nTPM
Skin	21.5 nTPM

Basal prostatic cells	92.7 nTPM
Prostate	92.7 nTPM

Basal respiratory cells	2.9 nTPM
Bronchus	2.9 nTPM

Basal squamous epithelial cells	4.6 nTPM
Esophagus	4.6 nTPM

Undifferentiated cells	0.4 nTPM
Colon	0.7 nTPM
Rectum	0.3 nTPM
Small intestine	0.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC