Single cell type - NDUFB5 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NDUFB5
Gene descriptionⁱ Full gene name according to HGNC.	NADH:ubiquinone oxidoreductase subunit B5
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Basic cellular processes (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NDUFB5 is part of cluster 39 Non-specific - Basic cellular processes with confidenceⁱ 1
586 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
UQCRFS1	Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1	0.7603	39
DLD	Dihydrolipoamide dehydrogenase	0.7381	39
NDUFA10	NADH:ubiquinone oxidoreductase subunit A10	0.7270	39
MRPS33	Mitochondrial ribosomal protein S33	0.7253	39
UQCRC2	Ubiquinol-cytochrome c reductase core protein 2	0.7225	39
LDHB	Lactate dehydrogenase B	0.6687	39
UQCRH	Ubiquinol-cytochrome c reductase hinge protein	0.6634	39
NTPCR	Nucleoside-triphosphatase, cancer-related	0.6578	39
PDHA1	Pyruvate dehydrogenase E1 subunit alpha 1	0.6539	39
COQ9	Coenzyme Q9	0.6246	39
PDHB	Pyruvate dehydrogenase E1 subunit beta	0.6216	39
COX7B	Cytochrome c oxidase subunit 7B	0.6215	39
UQCR10	Ubiquinol-cytochrome c reductase, complex III subunit X	0.6153	39
PRDX3	Peroxiredoxin 3	0.6138	39
NDUFA4	NDUFA4 mitochondrial complex associated	0.6115	39

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	113.6 nTPM
Breast	151.9 nTPM
Adipose tissue	75.4 nTPM

Alveolar cells type 1	48.1 nTPM
Lung	48.1 nTPM

Alveolar cells type 2	48.5 nTPM
Lung	48.5 nTPM

Astrocytes	17.3 nTPM
Brain	17.3 nTPM

B-cells	90.2 nTPM
Kidney	147.5 nTPM
PBMC	137.1 nTPM
Liver	106.7 nTPM
Colon	100.6 nTPM
Breast	77.5 nTPM
Spleen	71.3 nTPM
Bone marrow	67.9 nTPM
Adipose tissue	67.7 nTPM
Stomach	63.6 nTPM
Lymph node	61.7 nTPM

Dendritic cells	54.5 nTPM
Breast	70.3 nTPM
PBMC	59.8 nTPM
Adipose tissue	33.5 nTPM

Endothelial cells	89.2 nTPM
Placenta	149.5 nTPM
Testis	118.8 nTPM
Breast	117.1 nTPM
Liver	109.2 nTPM
Pancreas	104.2 nTPM
Skin	100.2 nTPM
Prostate	87.6 nTPM
Ovary	85.8 nTPM
Eye	82.5 nTPM
Endometrium	72.6 nTPM
Skeletal muscle	66.3 nTPM
Heart muscle	65.5 nTPM
Esophagus	55.6 nTPM
Lung	34.3 nTPM

Enteroendocrine cells	117.5 nTPM
Rectum	121.4 nTPM
Colon	113.7 nTPM

Fibroblasts	87.1 nTPM
Placenta	145.2 nTPM
Ovary	127.2 nTPM
Breast	111.7 nTPM
Heart muscle	104.0 nTPM
Adipose tissue	87.1 nTPM
Skin	81.5 nTPM
Lung	78.2 nTPM
Skeletal muscle	59.7 nTPM
Prostate	57.6 nTPM
Esophagus	56.4 nTPM
Stomach	49.0 nTPM

Intestinal goblet cells	196.0 nTPM
Rectum	230.2 nTPM
Colon	216.6 nTPM
Small intestine	141.2 nTPM

Macrophages	133.6 nTPM
Kidney	241.3 nTPM
Prostate	205.2 nTPM
Ovary	191.9 nTPM
Testis	181.2 nTPM
Pancreas	158.2 nTPM
Breast	141.2 nTPM
Spleen	139.9 nTPM
Bone marrow	137.4 nTPM
Adipose tissue	132.4 nTPM
Endometrium	106.2 nTPM
Skeletal muscle	91.6 nTPM
Lung	89.1 nTPM
Stomach	88.0 nTPM
Esophagus	59.8 nTPM
PBMC	40.9 nTPM

Paneth cells	163.4 nTPM
Rectum	218.4 nTPM
Small intestine	202.7 nTPM
Colon	69.2 nTPM

Plasma cells	75.0 nTPM
Spleen	104.9 nTPM
Stomach	90.0 nTPM
Bone marrow	87.4 nTPM
Esophagus	49.4 nTPM
Adipose tissue	43.2 nTPM

Respiratory ciliated cells	157.9 nTPM
Bronchus	259.0 nTPM
Lung	56.9 nTPM

Smooth muscle cells	98.5 nTPM
Ovary	167.5 nTPM
Pancreas	150.1 nTPM
Breast	124.4 nTPM
Bronchus	104.8 nTPM
Prostate	100.1 nTPM
Skin	85.7 nTPM
Skeletal muscle	80.7 nTPM
Adipose tissue	77.8 nTPM
Heart muscle	76.8 nTPM
Endometrium	74.0 nTPM
Esophagus	41.9 nTPM

Basal keratinocytes	85.1 nTPM
Skin	85.1 nTPM

Basal prostatic cells	115.2 nTPM
Prostate	115.2 nTPM

Basal respiratory cells	374.4 nTPM
Bronchus	374.4 nTPM

Basal squamous epithelial cells	73.4 nTPM
Esophagus	73.4 nTPM

T-cells	72.9 nTPM
Kidney	132.8 nTPM
PBMC	119.9 nTPM
Colon	107.4 nTPM
Ovary	102.3 nTPM
Liver	98.5 nTPM
Skin	91.7 nTPM
Breast	80.3 nTPM
Lymph node	65.0 nTPM
Spleen	61.6 nTPM
Stomach	58.9 nTPM
Prostate	56.8 nTPM
Bone marrow	55.4 nTPM
Adipose tissue	54.3 nTPM
Endometrium	43.7 nTPM
Lung	42.6 nTPM
Skeletal muscle	41.5 nTPM
Esophagus	26.2 nTPM

Undifferentiated cells	268.8 nTPM
Rectum	275.1 nTPM
Small intestine	274.5 nTPM
Colon	256.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC