Single cell type - MED8 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MED8
Gene descriptionⁱ Full gene name according to HGNC.	Mediator complex subunit 8
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

MED8 is part of cluster 10 Non-specific - Unknown function with confidenceⁱ 1
482 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
YIF1A	Yip1 interacting factor homolog A, membrane trafficking protein	0.5712	10
PSMA7	Proteasome 20S subunit alpha 7	0.5682	10
SLC35B1	Solute carrier family 35 member B1	0.5532	55
PDAP1	PDGFA associated protein 1	0.5509	10
PSMC2	Proteasome 26S subunit, ATPase 2	0.5483	10
VPS25	Vacuolar protein sorting 25 homolog	0.5479	10
UBE2V2	Ubiquitin conjugating enzyme E2 V2	0.5243	10
COPB2	COPI coat complex subunit beta 2	0.5155	55
PARL	Presenilin associated rhomboid like	0.5136	10
ERP44	Endoplasmic reticulum protein 44	0.5092	55
DUSP12	Dual specificity phosphatase 12	0.5064	10
ZMAT5	Zinc finger matrin-type 5	0.4975	10
REEP5	Receptor accessory protein 5	0.4973	10
EIF3H	Eukaryotic translation initiation factor 3 subunit H	0.4963	10
SRP72	Signal recognition particle 72	0.4936	55

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	25.6 nTPM
Breast	36.8 nTPM
Adipose tissue	14.3 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	12.9 nTPM
Lung	12.9 nTPM

Astrocytes	5.9 nTPM
Brain	5.9 nTPM

B-cells	25.8 nTPM
Liver	65.2 nTPM
Breast	46.8 nTPM
Stomach	28.2 nTPM
PBMC	27.1 nTPM
Bone marrow	22.1 nTPM
Kidney	19.0 nTPM
Spleen	16.2 nTPM
Colon	14.4 nTPM
Adipose tissue	14.1 nTPM
Lymph node	5.2 nTPM

Dendritic cells	26.2 nTPM
Breast	31.0 nTPM
PBMC	23.9 nTPM
Adipose tissue	23.5 nTPM

Endothelial cells	25.1 nTPM
Pancreas	61.8 nTPM
Prostate	35.0 nTPM
Placenta	28.4 nTPM
Ovary	26.7 nTPM
Esophagus	25.3 nTPM
Eye	24.2 nTPM
Testis	22.8 nTPM
Breast	21.9 nTPM
Liver	21.1 nTPM
Skin	21.1 nTPM
Skeletal muscle	19.7 nTPM
Endometrium	19.4 nTPM
Heart muscle	17.8 nTPM
Lung	6.2 nTPM

Fibroblasts	30.3 nTPM
Placenta	61.2 nTPM
Breast	38.9 nTPM
Prostate	35.7 nTPM
Ovary	32.5 nTPM
Lung	30.1 nTPM
Adipose tissue	27.1 nTPM
Stomach	26.7 nTPM
Skeletal muscle	22.6 nTPM
Esophagus	22.3 nTPM
Skin	19.6 nTPM
Heart muscle	17.1 nTPM

Intestinal goblet cells	29.2 nTPM
Colon	47.2 nTPM
Small intestine	21.7 nTPM
Rectum	18.8 nTPM

Macrophages	26.2 nTPM
Pancreas	71.5 nTPM
Ovary	50.5 nTPM
Testis	34.9 nTPM
Breast	32.7 nTPM
Kidney	32.5 nTPM
Lung	31.9 nTPM
Endometrium	27.3 nTPM
Bone marrow	25.0 nTPM
Adipose tissue	21.6 nTPM
Prostate	20.7 nTPM
Skeletal muscle	17.7 nTPM
Esophagus	9.4 nTPM
Spleen	9.3 nTPM
Stomach	8.8 nTPM
PBMC	0.0 nTPM

Paneth cells	30.0 nTPM
Small intestine	48.8 nTPM
Rectum	21.4 nTPM
Colon	19.7 nTPM

Plasma cells	41.2 nTPM
Spleen	61.8 nTPM
Stomach	53.3 nTPM
Bone marrow	46.2 nTPM
Esophagus	24.6 nTPM
Adipose tissue	20.0 nTPM

Respiratory ciliated cells	16.0 nTPM
Bronchus	23.2 nTPM
Lung	8.7 nTPM

Smooth muscle cells	42.9 nTPM
Bronchus	167.1 nTPM
Pancreas	109.9 nTPM
Ovary	34.3 nTPM
Breast	27.3 nTPM
Prostate	26.3 nTPM
Endometrium	20.4 nTPM
Adipose tissue	20.3 nTPM
Heart muscle	19.7 nTPM
Esophagus	17.0 nTPM
Skin	15.0 nTPM
Skeletal muscle	14.6 nTPM

T-cells	23.3 nTPM
Prostate	42.6 nTPM
Ovary	42.2 nTPM
Colon	32.3 nTPM
Kidney	30.7 nTPM
PBMC	29.7 nTPM
Lung	24.3 nTPM
Liver	23.7 nTPM
Breast	23.4 nTPM
Bone marrow	22.9 nTPM
Adipose tissue	21.9 nTPM
Stomach	20.5 nTPM
Skin	20.2 nTPM
Endometrium	19.9 nTPM
Spleen	15.0 nTPM
Skeletal muscle	12.9 nTPM
Lymph node	7.7 nTPM
Esophagus	6.6 nTPM

Basal keratinocytes	18.7 nTPM
Skin	18.7 nTPM

Basal respiratory cells	35.6 nTPM
Bronchus	35.6 nTPM

Basal squamous epithelial cells	11.4 nTPM
Esophagus	11.4 nTPM

Collecting duct cells	14.7 nTPM
Kidney	14.7 nTPM

Undifferentiated cells	22.6 nTPM
Colon	29.2 nTPM
Rectum	20.4 nTPM
Small intestine	18.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC